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Ki67 Antibody (YA717)

(Synonyms: Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67)
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Based on 1 publication(s) in Google Scholar

Ki67 Antibody (YA717) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Ki67.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Conjugation:
    Non-conjugated
  • Ki67 Antibody (YA717) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-1:2000

Product Details

Description

Ki67 Antibody (YA717) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Ki67.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 359 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 359 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Ki67.AA range:1040-1080.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102578

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    Ki67 is a Protein that associates with the surface of mitotic chromosomes and acts both as a chromosome repellent during early mitosis and chromosome attractant during late mitosis. Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. During early mitosis, relocalizes from nucleoli to the chromosome surface where it forms extended brush structures that cover a substantial fraction of the chromosome surface. The MKI67 brush structure prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. During mitotic anaphase, the MKI67 brush structure collapses and MKI67 switches from a chromosome repellent to a chromosome attractant to promote chromosome clustering and facilitate the exclusion of large cytoplasmic particles from the future nuclear space. Mechanistically, dephosphorylation during mitotic exit and simultaneous exposure of a conserved basic patch induce the RNA-dependent formation of a liquid-like condensed phase on the chromosome surface, promoting coalescence of neighboring chromosome surfaces and clustering of chromosomes. Binds premature ribosomal RNAs during anaphase; promoting liquid-liquid phase separation. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization; it is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in mitotic chromosome[1][2][3][4][5][6][7].

  • Subcellular Localization

    Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole; Golgi apparatus membrane; Peripheral membrane protein; Cell projection, bleb; Cytoplasm, cytoskeleton; Cell membrane; Cell projection, lamellipodium; Cell projection, ruffle

  • Expression


    Tissue_specificity:Detected in platelets

  • Subunit

    Homodimer. Interacts with RHOB, RHOC, MYLC2B and PTEN. Interacts with ITGB1BP1 (via N-terminus and PTB domain) (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Interacts with SHROOM3 (PubMed:22493320)

  • SwissProt ID

    P46013

  • Gene ID
    4288 [NCBI]
  • Synonyms

    Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

  • Research Field

    Cell Biology

[1]. Cuylen S, et al. Ki-67 acts as a biological surfactant to disperse mitotic chromosomes. Nature. 2016 Jul 14;535(7611):308-12.  [Content Brief]

[2]. Cuylen-Haering S, et al. Chromosome clustering by Ki-67 excludes cytoplasm during nuclear assembly. Nature. 2020 Nov;587(7833):285-290. [Content Brief]

[3]. Yamazaki H, et al. Cell cycle-specific phase separation regulated by protein charge blockiness. Nat Cell Biol. 2022 May;24(5):625-632.  [Content Brief]

[4]. Hernandez-Armendariz A, et al. A liquid-like coat mediates chromosome clustering during mitotic exit. Mol Cell. 2024 Sep 5;84(17):3254-3270.e9.  [Content Brief]

[5]. Hayashi Y, et al. The hierarchical structure of the perichromosomal layer comprises Ki67, ribosomal RNAs, and nucleolar proteins. Biochem Biophys Res Commun. 2017 Nov 18;493(2):1043-1049.  [Content Brief]

[6]. MacCallum DE, et al. The biochemical characterization of the DNA binding activity of pKi67. J Pathol. 2000 Jul;191(3):286-98.   [Content Brief]

[7]. Booth DG, et al. Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery. Elife. 2014 May 27;3:e01641.  [Content Brief]

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Ki67 Antibody (YA717) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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