KLF1 Antibody (YA4234)(PBS only)

Cat. No.: HY-P84537A: Data Sheet User Guide for Antibodies
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KLF1 Antibody (YA4234) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KLF1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

KLF1 Antibody (YA4234) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KLF1.

Host

Mouse

Molecular Weight

Predicted band size: 38.2 kDa

Species Reactivity

Human

SwissProt ID

Q13351

Gene ID

10661 [NCBI]

Immunogen

Purified recombinant fragment of human KLF1 (AA: 208-362) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters (PubMed:21055716). When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines

Subunit：Interacts with PCAF; the interaction does not acetylate EKLF and inhibits its transactivation activity (By similarity). Interacts with CREBBP/CBP and EP300; the interactions enhance the transactivation activity. Interacts with TFB1

Synonyms

EKLF; INLU; HBFQTL6

Documentation

User Guide for Antibodies (1077 KB)