Kallikrein 2 Antibody (YA3861)

(Synonyms: hK2; hGK-1; KLK2A2)
Customer Review

Based on 1 Customer Validation

Kallikrein 2 Antibody (YA3861) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Kallikrein 2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:50-1:200 1:200-1:400 1:10000

Product Details

Description

Kallikrein 2 Antibody (YA3861) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Kallikrein 2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 29 kDa
Immunogen

Purified recombinant fragment of human KLK2 (AA: 25-261) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718851

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Kallikrein 2 Antibody (YA3861)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using Kallikrein 2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84164, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Kallikrein 2 Antibody (YA3861)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using Kallikrein 2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84164, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Kallikrein 2 Antibody (YA3861)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using Kallikrein 2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84164, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Kallikrein 2 Antibody (YA3861)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using Kallikrein 2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84164, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Kallikrein 2 Antibody (YA3861)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using Kallikrein 2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84164, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Kallikrein 2 Antibody (YA3861)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer‌ tissue using Kallikrein 2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84164, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    Kallikrein 2 is a Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin

  • Isoforms & Post-Translational Modification

    P20151 has 4 isomers: P20151-1: 28671 Da (predicted); P20151-2: 24674 Da (predicted); P20151-3: 18162 Da (predicted); P20151-4: 17301 Da (predicted).

  • SwissProt ID

    P20151

  • Gene ID
  • Synonyms

    hK2; hGK-1; KLK2A2

Kallikrein 2 Antibody (YA3861) Related Classifications

MOQ
Minimum order quantity
100 mg

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