MBNL1 Antibody (YA7257)

Cat. No.: HY-P87573: Data Sheet User Guide for Antibodies
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MBNL1 Antibody (YA7257) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MBNL1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

MBNL1 Antibody (YA7257) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MBNL1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 42 kDa;

Observed band size: 40 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human Predicted Reactivity: Mouse

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

Q9NR56

Immunogen

A synthesized peptide derived from human MBNL1

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:50-100

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues. Together with RNA binding proteins RBPMS and RBFOX2, activates vascular smooth muscle cells alternative splicing events (PubMed:37548402). Regulates NCOR2 alternative splicing (By similarity)

Subcellular Localization：Nucleus,Cytoplasm,Cytoplasmic granule

Expression：
Tissue_Specificity: Highly expressed in cardiac, skeletal muscle and during myoblast differentiation. Weakly expressed in other tissues (at protein level). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas

Isoforms & Post-Translational Modification：Q9NR56 has 7 isomers: Q9NR56-1: 41817 Da (predicted); Q9NR56-2: 39831 Da (predicted); Q9NR56-3: 33050 Da (predicted); Q9NR56-4: 34211 Da (predicted); Q9NR56-5: 40992 Da (predicted); Q9NR56-6: 37074 Da (predicted); Q9NR56-7: 36992 Da (predicted).

Subunit：Interacts with DDX1 and YBX1

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/sf21-cell.html [Content Brief]