MICB Antibody (YA1466)
(Synonyms: MIC-B; PERB11.2)MICB Antibody (YA1466) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MICB.
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Host:
Rabbit
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Isotype:
IgG
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Application:
FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:50-1:100 | 1:10000 |
Product Details
MICB Antibody (YA1466) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MICB.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 60 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 43 kDa
Recombinant human MICB protein
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MICB is widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8+ alphabeta T-cells. Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production[1][2].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein
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Expression
Tissue_specificity:Besides the central nervous system, this gene is widely expressed in other tissues. It is expressed in various epithelial tumors, including lung cancer, breast cancer, kidney cancer, ovarian cancer, prostate cancer, and colon cancer, but not in all epithelial tumors. In hepatocellular carcinoma, this gene is expressed in tumor cells but not in surrounding non-cancerous tissues.
Induction:By heat shock, oxidative stress, retinoic acid, IFN-alpha and the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine. Induction by IFN-alpha is impaired with chronic hepatitis C virus infection. Down-regulated by human cytomegalovirus UL112 microRNA during viral infection which leads to decreased binding of KLRK1/NKG2D and reduced killing by natural killer cells -
Isoforms & Post-Translational Modification
Q29980 has 3 isomers: Q29980-1: 42575 Da (predicted); Q29980-2: 37580 Da (predicted); Q29980-3: 4287 Da (predicted).
Proteolytically cleaved and released from the cell surface of tumor cells -
Subunit
Unlike classical MHC class I molecules, does not form a heterodimer with beta-2-microglobulin. Binds as a monomer to a KLRK1/NKG2D homodimer. KLRK1 forms a complex with HCST/DAP10 in which KLRK1 binds MICB while HCST acts as an adapter molecule which enables signal transduction. Receptor-ligand interaction induces clustering of both proteins in ordered structures called immune synapses and also leads to their intercellular transfer. This is associated with a reduction in the cytotoxicity of KLRK1-expressing cells
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SwissProt ID
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Synonyms
MIC-B; PERB11.2
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Research Field
Immunology
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