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MRP3 Antibody (YA3529)

(Synonyms: MLP2; MRP3; ABC31; MOAT-D; cMOAT2; EST90757)
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Based on 1 Customer Validation

MRP3 Antibody (YA3529) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MRP3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MRP3 Antibody (YA3529)
  • MRP3 Antibody (YA3529) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

MRP3 Antibody (YA3529) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MRP3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 170 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Immunogen

Purified recombinant fragment of human MRP3 (AA: 830-949) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MRP3 Antibody (YA3529)
    Flow cytometric analysis of 1X10^6 HT-29 cells labeling MRP3 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    MRP3 is an ATP-dependent transporter of the ATP-binding cassette (ABC) family that binds and hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants and endogenous compound across cell membranes. Transports glucuronide conjugates such as bilirubin diglucuronide, estradiol-17-beta-o-glucuronide and GSH conjugates such as leukotriene C4 (LTC4). Transports also various bile salts (taurocholate, glycocholate, taurochenodeoxycholate-3-sulfate, taurolithocholate- 3-sulfate). Does not contribute substantially to bile salt physiology but provides an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes. May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (Probable). Can confer resistance to various anticancer drugs, methotrexate, tenoposide and etoposide, by decreasing accumulation of these drugs in cells[1][2][3].

  • Subcellular Localization

    Basolateral cell membrane; Multi-pass membrane protein; Basal cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Mainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney. In testis, localized to peritubular myoid cells, Leydig cells, along the basal membrane of Sertoli cells and moderately in the adluminal compartment of the seminiferous tubules (PubMed:35307651)

    Induction:Strongly up-regulated under conditions of MRP2 deficiency

  • Isoforms & Post-Translational Modification

    O15438 has 5 isomers: O15438-1: 169343 Da (predicted); O15438-2: 137297 Da (predicted); O15438-3: 55305 Da (predicted); O15438-4: 174907 Da (predicted); O15438-5: 64632 Da (predicted).

  • SwissProt ID

    O15438

  • Gene ID
    8714 [NCBI]
  • Synonyms

    MLP2; MRP3; ABC31; MOAT-D; cMOAT2; EST90757

MRP3 Antibody (YA3529) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HT-29 cells labeling MRP3 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MRP3 Antibody (YA3529) Product Image
    1/16
  • Experimental Validation Results for MRP3 Antibody (YA3529)
  • MRP3 Antibody (YA3529) Product Image
  • Experimental Validation Results for MRP3 Antibody (YA3529)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HT-29 cells labeling MRP3 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for MRP3 Antibody (YA3529)
  • MRP3 Antibody (YA3529) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 HT-29 cells labeling MRP3 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MRP3 Antibody (YA3529) Product Image FC