Neurofilament Medium Antibody (YA3680)
(Synonyms: NFM; NEF3; NF-M)
Based on 1 Customer Validation
Neurofilament Medium Antibody (YA3680) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Neurofilament Medium.
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Host:
Mouse
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Isotype:
IgG
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Application:
ICC/IF, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:50-1:200 | 1:200-1:400 | 1:10000 |
Product Details
Neurofilament Medium Antibody (YA3680) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Neurofilament Medium.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 160 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 102 kDa
Purified recombinant fragment of human NEFM (AA: 779-916) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Neurofilament Medium is a Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks
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Subcellular Localization
Cytoplasm, cytoskeleton; Cell projection, axon
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Isoforms & Post-Translational Modification
P07197 has 2 isomers: P07197-1: 102472 Da (predicted); P07197-2: 59468 Da (predicted).
There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber;Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function;Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization -
Subunit
Forms heterodimers with NEFL; which can further hetero-oligomerize (in vitro) (By similarity). Forms heterodimers with INA (in vitro) (By similarity)
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SwissProt ID
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Synonyms
NFM; NEF3; NF-M
Documentation
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