NMNAT1 Antibody (YA8263)
(Synonyms: LCA9; NMNAT; PNAT1; SHILCA)NMNAT1 Antibody (YA8263) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NMNAT1.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P, FC
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Reactivity :
Human
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|
| Dilution Ratio | 1:150-500 | 1:100 |
Product Details
NMNAT1 Antibody (YA8263) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NMNAT1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 31.8 kDa
Full length human recombinant protein of human NMNAT1 produced in HEK293T cell.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NMNAT1 catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming. Also acts as a cofactor for glutamate and aspartate ADP-ribosylation by directing PARP1 catalytic activity to glutamate and aspartate residues on histones. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following mechanical or toxic insults. Neural protection does not correlate with cellular NAD(+) levels but may still require enzyme activity[1][2].
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Subcellular Localization
Nucleus
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Expression
Tissue_Specificity: Widely expressed with highest levels in skeletal muscle, heart and kidney. Also expressed in the liver pancreas and placenta. Widely expressed throughout the brain -
Isoforms & Post-Translational Modification
Q9HAN9: 279 amino acids, molecular weight 31932 Da.
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Subunit
Homohexamer (PubMed:11751893)
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SwissProt ID
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Synonyms
LCA9; NMNAT; PNAT1; SHILCA
Documentation
References
[1]. Sorci L, et al. Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrate analogues, and isozyme contributions to NAD+ biosynthesis. Biochemistry. 2007 Apr 24;46(16):4912-22. [Content Brief]
[2]. Wright RH, et al. ADP-ribose-derived nuclear ATP synthesis by NUDIX5 is required for chromatin remodeling. Science. 2016 Jun 3;352(6290):1221-5. [Content Brief]
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