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NOTCH4 Antibody (YA4443)

(Synonyms: INT3)
Customer Review

Based on 1 Customer Validation

NOTCH4 Antibody (YA4443) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to NOTCH4.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL IHC-P FC
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • NOTCH4 Antibody (YA4443) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

NOTCH4 Antibody (YA4443) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to NOTCH4.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 230 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 210 kDa
Immunogen

Purified recombinant fragment of human NOTCH4 (AA: extra 118-357) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

RRID

AB_3718976

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    Immunohistochemical analysis of paraffin-embedded human Lung tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    Immunohistochemical analysis of paraffin-embedded human Lung tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    Immunohistochemical analysis of paraffin-embedded human Lung tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    Immunohistochemical analysis of paraffin-embedded human heart tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    Immunohistochemical analysis of paraffin-embedded human heart tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    Immunohistochemical analysis of paraffin-embedded human heart tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    Flow cytometric analysis of 1X106 Jurkat cells labeling NOTCH4 Antibody(HY-P84746, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    NOTCH4 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Nucleus

  • Expression


    Tissue_specificity:This gene is highly expressed in the heart, moderately expressed in the lungs and placenta, and lowly expressed in the liver, skeletal muscle, kidneys, pancreas, spleen, lymph nodes, thymus, bone marrow, and fetal liver. It is not expressed in adult brain tissue or peripheral blood leukocytes.

  • Isoforms & Post-Translational Modification

    Q99466 has 3 isomers: Q99466-1: 209622 Da (predicted); Q99466-2: 61262 Da (predicted); Q99466-3: 39617 Da (predicted).
    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity);Phosphorylated

  • Subunit

    Heterodimer of a C-terminal fragment N(TM) and a N-terminal fragment N(EC) which are probably linked by disulfide bonds (By similarity). Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4

  • SwissProt ID

    Q99466

  • Gene ID
    4855 [NCBI]
  • Synonyms

    INT3

NOTCH4 Antibody (YA4443) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling NOTCH4 Antibody(HY-P84746, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • NOTCH4 Antibody (YA4443) Product Image
    1/16
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • NOTCH4 Antibody (YA4443) Product Image
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling NOTCH4 Antibody(HY-P84746, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • Experimental Validation Results for NOTCH4 Antibody (YA4443)
  • NOTCH4 Antibody (YA4443) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NOTCH4 Antibody (YA4443) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NOTCH4 Antibody (YA4443) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NOTCH4 Antibody (YA4443) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NOTCH4 Antibody (YA4443) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NOTCH4 Antibody (YA4443) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NOTCH4 Antibody (YA4443) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NOTCH4 Antibody (YA4443) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NOTCH4 Antibody (YA4443) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NOTCH4 Antibody (YA4443) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NOTCH4 Antibody (YA4443) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NOTCH4 Antibody (YA4443) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using NOTCH4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84746, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NOTCH4 Antibody (YA4443) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 Jurkat cells labeling NOTCH4 Antibody(HY-P84746, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • NOTCH4 Antibody (YA4443) Product Image IHC-P
  • NOTCH4 Antibody (YA4443) Product Image IHC-P
  • NOTCH4 Antibody (YA4443) Product Image IHC-P
  • NOTCH4 Antibody (YA4443) Product Image IHC-P
  • NOTCH4 Antibody (YA4443) Product Image IHC-P
  • NOTCH4 Antibody (YA4443) Product Image IHC-P
  • NOTCH4 Antibody (YA4443) Product Image IHC-P
  • NOTCH4 Antibody (YA4443) Product Image IHC-P
  • NOTCH4 Antibody (YA4443) Product Image IHC-P
  • NOTCH4 Antibody (YA4443) Product Image IHC-P
  • NOTCH4 Antibody (YA4443) Product Image IHC-P
  • NOTCH4 Antibody (YA4443) Product Image IHC-P
  • NOTCH4 Antibody (YA4443) Product Image FC