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NPM2 Antibody (YA4353)

Customer Review

Based on 1 Customer Validation

NPM2 Antibody (YA4353) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NPM2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • NPM2 Antibody (YA4353) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

NPM2 Antibody (YA4353) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NPM2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 24.1 kDa
Immunogen

Purified recombinant fragment of human NPM2 (AA: 1-214) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718957

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NPM2 Antibody (YA4353)
    Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NPM2 Antibody (YA4353)
    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NPM2 Antibody (YA4353)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NPM2 Antibody (YA4353)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NPM2 Antibody (YA4353)
    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NPM2 Antibody (YA4353)
    Immunohistochemical analysis of paraffin-embedded human Liver cancer tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    NPM2 core histones chaperone involved in chromatin reprogramming, specially during fertilization and early embryonic development. Probably involved in sperm DNA decondensation during fertilization

  • Subcellular Localization

    Nucleus

  • Subunit

    Homopentamer, when bound to H2A-H2B dimers only. Homodecamer of two stacked pentamers, when bound to H2A-H2B dimers and H3-H4 tetramers simultaneously

  • SwissProt ID

    Q86SE8

  • Gene ID
    10361 [NCBI]

NPM2 Antibody (YA4353) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cancer tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NPM2 Antibody (YA4353) Product Image
    1/16
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • NPM2 Antibody (YA4353) Product Image
  • Experimental Validation Results for NPM2 Antibody (YA4353)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NPM2 Antibody (YA4353)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NPM2 Antibody (YA4353)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NPM2 Antibody (YA4353)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NPM2 Antibody (YA4353)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NPM2 Antibody (YA4353)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cancer tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • Experimental Validation Results for NPM2 Antibody (YA4353)
  • NPM2 Antibody (YA4353) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NPM2 Antibody (YA4353) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NPM2 Antibody (YA4353) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NPM2 Antibody (YA4353) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NPM2 Antibody (YA4353) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NPM2 Antibody (YA4353) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Liver cancer tissue using NPM2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84656, 1:200 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NPM2 Antibody (YA4353) Product Image IHC-P
  • NPM2 Antibody (YA4353) Product Image IHC-P
  • NPM2 Antibody (YA4353) Product Image IHC-P
  • NPM2 Antibody (YA4353) Product Image IHC-P
  • NPM2 Antibody (YA4353) Product Image IHC-P
  • NPM2 Antibody (YA4353) Product Image IHC-P