NPM3 Antibody (YA3562)
(Synonyms: PORMIN; TMEM123)
Based on 1 Customer Validation
NPM3 Antibody (YA3562) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NPM3.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:50-1:200 | 1:200-1:400 | 1:10000 |
Product Details
NPM3 Antibody (YA3562) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NPM3.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 19 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 19 kDa
Purified recombinant fragment of human NPM3 (AA: full 1-178) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NPM3 plays a role in the regulation of diverse cellular processes such as ribosome biogenesis, chromatin remodeling or protein chaperoning. Modulates the histone chaperone function and the RNA-binding activity of nucleolar phosphoprotein B23/NPM. Efficiently mediates chromatin remodeling when included in a pentamer containing NPM3 and NPM[1][2][3].
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Subcellular Localization
Nucleus; Nucleus, nucleolus
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Expression
Tissue_specificity:Ubiquitous -
Subunit
Interacts with NPM (via N-terminus) (PubMed:15596447). Forms a pentamer with NPM at a ratio 4:1 (NPM3/NPM). Two pentamers form a decamer (PubMed:22362753)
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SwissProt ID
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Synonyms
PORMIN; TMEM123
Documentation
References
[1]. Gadad SS, et al. NPM3, a member of the nucleophosmin/nucleoplasmin family, enhances activator-dependent transcription. Biochemistry. 2010 Feb 23;49(7):1355-7. [Content Brief]
[2]. Okuwaki M, et al. Function of homo- and hetero-oligomers of human nucleoplasmin/nucleophosmin family proteins NPM1, NPM2 and NPM3 during sperm chromatin remodeling. Nucleic Acids Res. 2012 Jun;40(11):4861-78. [Content Brief]
[3]. Huang N, et al. Protein NPM3 interacts with the multifunctional nucleolar protein B23/nucleophosmin and inhibits ribosome biogenesis. J Biol Chem. 2005 Feb 18;280(7):5496-502. [Content Brief]
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