Oct-4 Antibody (YA4160)
(Synonyms: OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4)Oct-4 Antibody (YA4160) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Oct-4.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
Oct-4 Antibody (YA4160) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Oct-4.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 45 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 39 kDa
Purified recombinant fragment of human (AA: 136-360) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
2026-10-04 00:00:00 transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency
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Subcellular Localization
Cytoplasm; Nucleus
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Expression
Tissue_specificity:It is expressed in the developing brain. The highest expression levels are found in specific cell layers of the cerebral cortex, olfactory bulb, hippocampus, and cerebellum. Expression levels are lower in adult tissues.
Induction:Transcriptional activity is positively regulated by PKM (PubMed:18191611) . Upon cell differentiation, expression is repressed by NR6A1 (PubMed:26769970) -
Isoforms & Post-Translational Modification
Q01860 has 2 isomers: Q01860-1: 38571 Da (predicted); Q01860-2: 30085 Da (predicted).
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression;Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation;ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription -
Subunit
Interacts with PKM. Interacts with WWP2. Interacts with UBE2I and ZSCAN10 (By similarity). Interacts with PCGF1 (PubMed:26687479). Interacts with ESRRB; recruits ESRRB near the POU5F1-SOX2 element in the NANOG proximal promoter; the interaction is DNA independent (By similarity). Interacts with ZNF322 (By similarity). Interacts with MAPK8 and MAPK9; the interaction allows MAPK8 and MAPK9 to phosphorylate POU5F1 on Ser-355 (By similarity). Interacts (when phosphorylated on Ser-355) with FBXW8 (By similarity). Interacts with FBXW4 (By similarity). Interacts with SOX2 and SOX15; binds synergistically with either SOX2 or SOX15 to DNA (By similarity). Interacts with DDX56 (By similarity)
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SwissProt ID
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Synonyms
OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
Documentation
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