Prostatic Acid Phosphatase Antibody (YA5737)

Cat. No.: HY-P86045: Data Sheet User Guide for Antibodies
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Prostatic Acid Phosphatase Antibody (YA5737) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Prostatic Acid Phosphatase.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Prostatic Acid Phosphatase Antibody (YA5737) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Prostatic Acid Phosphatase.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 58 kDa;

Observed band size: 68 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P15309

Gene ID

55 [NCBI]

Immunogen

Synthesized peptide derived from human PSAP AA range: 201-300

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:500-5000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins (PubMed:10506173, PubMed:15280042, PubMed:20498373, PubMed:9584846). Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma (PubMed:10506173, PubMed:15280042); Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling (PubMed:20498373). In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor (By similarity); (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection (PubMed:18083097, PubMed:19451623, PubMed:19897482). SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea (PubMed:19451623). Target cell attachment and enhancement of HIV infection is inhibited by surfen (PubMed:19897482). Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection (PubMed:19403677)

Subcellular Localization：Secreted; Cell membrane; Single-pass type I membrane protein; Lysosome membrane; Single-pass type I membrane protein; Nucleus; Cytoplasm, cytosol

Expression：
Tissue_specificity:It is highly expressed in the prostate, limited to glandular and ductal epithelial cells. It is also expressed in the bladder, kidneys, pancreas, lungs, cervix, testes, and ovaries. It is weakly expressed in some pancreatic islet cells, squamous epithelium, pilosebaceous units, colonic neuroendocrine cells, and skin appendage structures. It is expressed at low levels in prostate cancer cells and tissues; it is also widely expressed. It is expressed in the sarcolemma of skeletal muscle.

Isoforms & Post-Translational Modification：P15309 has 3 isomers: P15309-1: 44566 Da (predicted); P15309-2: 48336 Da (predicted); P15309-3: 40443 Da (predicted).
N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides;Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection)

Subunit：Homodimer; dimer formation is required for phosphatase activity

Synonyms

Prostatic acid phosphatase; PAP; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; Thiamine monophosphatase; TMPase; [Cleaved into: PAPf39]

Documentation

User Guide for Antibodies (1077 KB)