PSMD10 Antibody (YA7788)(PBS only)

Cat. No.: HY-P88104A: Data Sheet User Guide for Antibodies
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PSMD10 Antibody (YA7788) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PSMD10.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PSMD10 Antibody (YA7788) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PSMD10.

Host

Mouse

Molecular Weight

Predicted band size: 24.4 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O75832

Immunogen

Full length human recombinant protein of human PSMD10 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis|Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export

Subcellular Localization：Cytoplasm,Nucleus

Expression：
Tissue_Specificity: Tends to be up-regulated in cancer cells with RAS mutations, including lung cancers and adenocarconimas (at protein level)

Isoforms & Post-Translational Modification：O75832 has two isomers: O75832-1: 24428 Da (predicted); O75832-2: 16137 Da (predicted).

Subunit：Part of transient complex containing PSMD10, PSMC4, PSMC5 and PAAF1 formed during the assembly of the 26S proteasome

Synonyms

dJ889N15.2; p28; p28(GANK)

Documentation

User Guide for Antibodies (1077 KB)