RAP80 Antibody (YA4438)(PBS only)
(Synonyms: RAP80, RXRIP110, UIMC1, BRCA1-A complex subunit RAP80, Receptor-associated protein 80, Retinoid X receptor-interacting protein 110, Ubiquitin interaction motif-containing protein 1)RAP80 Antibody (YA4438) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RAP80.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:50-1:250 FC: 1:200-1:400 | 1:10000 |
Product Details
RAP80 Antibody (YA4438) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RAP80.
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Host Mouse
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 80 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 80 kDa
Purified recombinant fragment of Human herpesvirus UL37 (AA: 970-1119) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Ubiquitin-binding protein (PubMed:24627472). Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref.38). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:It is expressed in the testes, ovaries, thymus, and heart. It is also expressed in testicular germ cells. -
Isoforms & Post-Translational Modification
Q96RL1 has 5 isomers: Q96RL1-1: 79727 Da (predicted); Q96RL1-2: 61325 Da (predicted); Q96RL1-3: 70744 Da (predicted); Q96RL1-4: 38477 Da (predicted); Q96RL1-5: 17412 Da (predicted).
Sumoylated;Phosphorylated upon DNA damage by ATM or ATR -
Subunit
Component of the ARISC complex, at least composed of UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1 (PubMed:24075985).
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SwissProt ID
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Synonyms
RAP80, RXRIP110, UIMC1, BRCA1-A complex subunit RAP80, Receptor-associated protein 80, Retinoid X receptor-interacting protein 110, Ubiquitin interaction motif-containing protein 1
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