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Cat. No.: HY-P87530

RHEB Antibody (YA7214)

RHEB Antibody (YA7214) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RHEB.

For research use only. We do not sell to patients.

Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	IHC-P IHC-P: Immunohistochemistry-Paraffin	IHC-F IHC-F: Immunohistochemistry-Frozen	IF-Tissue IF-Tissue: Immunofluorescence-Tissue	FC FC: Flow Cytometry	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence
Dilution Ratio	1:100-200	1:100-200	1:50-200	1:50-100	1:50-200

Description

RHEB Antibody (YA7214) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RHEB.

Host Rabbit
Clonality Recombinant,Monoclonal
Observed Molecular Weight

Observed band size: 20 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 20 kDa

Immunogen

A synthesized peptide derived from human RHEB

Sensitivity

Endogenous

Purification

affinity purified by Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Appearance

Liquid
Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Function

RHEB is a Small GTPase that acts as an allosteric activator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. In response to nutrients, growth factors or amino acids, specifically activates the protein kinase activity of MTOR, the catalytic component of the mTORC1 complex: acts by causing a conformational change that allows the alignment of residues in the active site of MTOR, thereby enhancing the phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). RHEB is also required for localization of the TSC-TBC complex to lysosomal membranes. In response to starvation, RHEB is inactivated by the TSC-TBC complex, preventing activation of mTORC1. Has low intrinsic GTPase activity^[1]^[2]^[3]^[4]^[5]^[6]^[7]^[8]^[9]^[10]^[11]^[12]^[13]^[14]^[15]^[16].
Subcellular Localization

Endomembrane system,Lysosome membrane,Golgi apparatus membrane,Endoplasmic reticulum membrane,Cytoplasm, cytosol
Expression

Tissue_Specificity: Ubiquitous (PubMed:8543055). Highest levels observed in skeletal and cardiac muscle (PubMed:8543055)
Isoforms & Post-Translational Modification

Q15382: 184 amino acids, molecular weight 20497 Da.
Subunit

Associates with the mTORC1 complex (MTOR, MLST8 and RPTOR) in a guanyl nucleotide-independent manner (PubMed:15854902, PubMed:16098514, PubMed:24529379)
SwissProt ID

Q15382