RUNX3 Antibody (YA4253)
(Synonyms: AML2; CBFA3; PEBP2aC; FLJ34510; MGC16070)
Based on 1 Customer Validation
RUNX3 Antibody (YA4253) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to RUNX3.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
RUNX3 Antibody (YA4253) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to RUNX3.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 44 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 44 kDa
Purified recombinant fragment of human RUNX3 (AA:186-252) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG2b
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Flow cytometric analysis of 1X10^6 NIH3T3 cells labeling RUNX3 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
Background
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Function
RUNX3 is a Runt-domain transcription factor that regulates gene expression through conserved RUNX-family DNA-binding architecture[1]. Mechanistically, RUNX3 supports TGF-β/SMAD-dependent growth control in gastric epithelium, where Runx3 loss causes epithelial hyperplasia, increased proliferation, reduced apoptosis, and resistance to TGF-β responses[2][3]. RUNX3 also restrains Wnt/β-catenin/TCF signaling in intestinal tumorigenesis by forming a complex with β-catenin/TCF4 and reducing Wnt target activity[4]. In immune models, Runx3 establishes CD8+ tissue-resident memory T-cell differentiation, supports tissue-residency genes, suppresses tissue-egress genes, and improves tumor-specific CD8+ T-cell abundance in mouse tumors[5]. Compared with related RUNX isoforms, RUNX3 is the smallest mammalian RUNX-family gene, while RUNX1, RUNX2, and RUNX3 share DNA-binding motifs and require regulated temporal and spatial expression[1]. RUNX3 isoform biology is experimentally important because P1 and P2 promoters regulate RUNX3 expression in a cell-type-specific manner[1]. In ovarian carcinoma models, RUNX3 transcript variants show distinct effects on platinum sensitivity, DNA repair, angiogenesis, and malignant phenotypes[6].- RUNX3 links transcriptional control, TGF-β signaling, Wnt suppression, and immune residency programs.- Isoform-specific RUNX3 models support studies of platinum response, angiogenesis, and tumor biology.
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Subcellular Localization
Nucleus; Cytoplasm
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Expression
Tissue_specificity:Expression (protein level) in gastric cancer tissue -
Isoforms & Post-Translational Modification
Q13761 has 2 isomers: Q13761-1: 44356 Da (predicted); Q13761-2: 45922 Da (predicted).
Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN -
Subunit
Heterodimer with CBFB. Interacts with TLE1 and SUV39H1 (PubMed:16652147, PubMed:9751710).
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SwissProt ID
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Synonyms
AML2; CBFA3; PEBP2aC; FLJ34510; MGC16070
Documentation
[1]. Bangsow C, et al. The RUNX3 gene--sequence, structure and regulated expression. Gene. 2001 Nov 28;279(2):221-32. [Content Brief]
[2]. Li QL, et al. Causal relationship between the loss of RUNX3 expression and gastric cancer. Cell. 2002 Apr 5;109(1):113-24. [Content Brief]
[3]. Chi XZ, et al. RUNX3 suppresses gastric epithelial cell growth by inducing p21(WAF1/Cip1) expression in cooperation with transforming growth factor {beta}-activated SMAD. Mol Cell Biol. 2005 Sep;25(18):8097-107. [Content Brief]
[4]. Ito K, et al. RUNX3 attenuates beta-catenin/T cell factors in intestinal tumorigenesis. Cancer Cell. 2008 Sep 9;14(3):226-37. [Content Brief]
[5]. Milner JJ, et al. Runx3 programs CD8+ T cell residency in non-lymphoid tissues and tumours. Nature. 2017 Dec 14;552(7684):253-257. [Content Brief]
[6]. Heinze K, et al. RUNX3 Transcript Variants Have Distinct Roles in Ovarian Carcinoma and Differently Influence Platinum Sensitivity and Angiogenesis. Cancers (Basel). 2021 Jan 26;13(3):476. [Content Brief]
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