S100A4 Antibody (YA5694)
(Synonyms: CAPL, MTS1, S100A4, Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4)
Based on 1 Customer Validation
S100A4 Antibody (YA5694) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to S100A4.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P, ICC/IF, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-400 | 1:50-200 | 1:500-5000 |
Product Details
S100A4 Antibody (YA5694) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to S100A4.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 12 kDa;
Synthesized peptide derived from human S100A4 AA range: 50-101
affinity chromatography.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
S100A4 is a member of the S100 family of low molecular weight calcium-binding proteins that modulate intracellular and extracellular signaling processes[1]. Mechanistically, S100A4 interacts with cytoskeletal elements, including actin and non-muscle myosin, to regulate cell motility, migration, and invasion, contributing to tissue remodeling and metastasis[2][3]. In disease models, S100A4 expression is elevated in fibrotic tissues, various cancers, and neurological disorders, where it promotes progression by activating RAGE-mediated pathways and influencing epithelial-mesenchymal transition[1][3]. Compared with related isoforms such as S100A1 and S100B, S100A4 exhibits lower basal expression in normal tissues but higher inducibility under pathological stress, indicating isoform-specific regulation[1][4]. Experimental inhibition of S100A4 using small-molecule antagonists or peptide-based inhibitors suppresses motility and metastasis in vitro and in vivo, highlighting its utility as a target for translational research[1][5]. For experimental applications, S100A4 can serve as a biomarker for tumor aggressiveness and as a modulator of fibroblast and immune cell function in tissue remodeling assays[1][2]. Collectively, S100A4 represents a distinct functional isoform within the S100 family, integrating calcium-dependent signaling with cytoskeletal regulation and disease pathogenesis.
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Subcellular Localization
Secreted; Nucleus; Cytoplasm
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Expression
Tissue_specificity:general expression -
Subunit
Homodimer. Interacts with PPFIBP1 in a calcium-dependent mode (PubMed:11836260). Interacts with PGLYRP1; this complex acts as a chemoattractant that promotes lymphocyte movement (PubMed:26654597, PubMed:30713770). Interacts with MYH9; this interaction increases cell motility (PubMed:16707441). Interacts with Annexin 2/ANXA2 (PubMed:28669632). Interacts with TP53; this interaction promotes TP53 degradation (PubMed:23752197, PubMed:32442400). Interacts with CCR5 (PubMed:30713770). Interacts with FCGR3A; this interaction inhibits PKC-dependent phosphorylation of FCGR3A
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SwissProt ID
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Synonyms
CAPL, MTS1, S100A4, Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4
Documentation
[1]. Delangre E, et al. S100 Proteins in Fatty Liver Disease and Hepatocellular Carcinoma. Int J Mol Sci. 2022 Sep 20;23(19):11030. [Content Brief]
[2]. Kraus C, et al. S100A1 in cardiovascular health and disease: closing the gap between basic science and clinical therapy. J Mol Cell Cardiol. 2009 Oct;47(4):445-55. [Content Brief]
[3]. Wilder PT, et al. Targeting S100 Calcium-Binding Proteins with Small Molecule Inhibitors. Methods Mol Biol. 2019;1929:291-310. [Content Brief]
[5]. Martínez-Aguilar J, et al. A multiplexed, targeted mass spectrometry assay of the S100 protein family uncovers the isoform-specific expression in thyroid tumours. BMC Cancer. 2015;15:199. [Content Brief]
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