S100A6 Antibody (YA5709)
(Synonyms: CACY, S100A6, Protein S100-A6, Calcyclin, Growth factor-inducible protein 2A9, MLN 4, Prolactin receptor-associated protein, S100 calcium-binding protein A6, PRA)
Based on 1 Customer Validation
S100A6 Antibody (YA5709) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to S100A6.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P, ICC/IF, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-400 | 1:50-200 | 1:500-5000 |
Product Details
S100A6 Antibody (YA5709) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to S100A6.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 10 kDa;
Synthesized peptide derived from human S100A6 AA range: 50-90
affinity chromatography.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Immunohistochemical analysis of paraffin-embedded human colon cancer using S100A6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86017, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human bladder cancer using S100A6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86017, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast cancer using S100A6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86017, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon using S100A6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86017, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human prostate using S100A6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86017, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney using S100A6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86017, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using S100A6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86017, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Background
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Function
S100A6 (calcyclin) is a member of the S100 calcium-binding protein family and contains two EF-hand calcium-binding motifs that enable calcium-dependent conformational changes and interactions with numerous target proteins involved in cellular signaling[1][2]. Upon Ca2+ binding, S100A6 exposes hydrophobic surfaces that facilitate interactions with proteins located in the cytoplasm, nucleus, cell membrane, and extracellular environment, thereby contributing to cytoskeletal regulation, signal transduction, cellular stress responses, proliferation, and differentiation[2][3]. Mechanistically, S100A6 participates in the regulation of cell-cycle progression and cytoskeletal dynamics through interactions with multiple binding partners, and its expression is preferentially induced when quiescent cells re-enter proliferation[3][4]. In disease contexts, aberrant S100A6 expression has been reported in numerous malignancies, including melanoma, lung, colorectal, pancreatic, liver, and thyroid cancers, supporting its relevance as a disease-associated biomarker and experimental target[2][5]. S100A6 is also implicated in non-neoplastic disorders, including nervous system, cardiovascular, and musculoskeletal diseases, indicating broad biological significance beyond tumor biology[2]. Compared with related S100 family isoforms, S100A6 exhibits distinct calcium-dependent interaction networks and subcellular localization patterns, including association with the plasma membrane and nuclear envelope following calcium activation[1][2]. For experimental applications, S100A6 is widely studied as a calcium-responsive regulatory protein and biomarker candidate; however, the retrieved literature does not provide well-established selective agonists or inhibitors suitable for routine research use[1][2].
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Subcellular Localization
Nucleus envelope; Cytoplasm; Cell membrane; Peripheral membrane protein; Cytoplasmic side
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Expression
Induction:Preferentially expressed when quiescent fibroblasts are stimulated to proliferate. It is inducible by growth factors and overexpressed in acute myeloid leukemias -
Subunit
Homodimer; head to tail assembly of 2 subunits. Interacts with CACYBP in a calcium-dependent manner. Interacts with ANXA2 and ANXA11 (via N-terminus). Interacts with SUGT1. Interacts with TP53; has higher affinity for TP53 that is phosphorylated on its N-terminal domain, and lower affinity for TP53 that is phosphorylated on its C-terminal domain. Interacts with tropomyosin. Interacts with FKBP4. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity. Interacts with TPPP; this interaction inhibits TPPP dimerization (PubMed:33831707)
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SwissProt ID
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Synonyms
CACY, S100A6, Protein S100-A6, Calcyclin, Growth factor-inducible protein 2A9, MLN 4, Prolactin receptor-associated protein, S100 calcium-binding protein A6, PRA
Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
[1]. Leśniak W, et al. S100A6 Protein-Expression and Function in Norm and Pathology. Int J Mol Sci. 2023 Jan 10;24(2):1341. [Content Brief]
[2]. Wang Y, et al. S100A6: molecular function and biomarker role. Biomark Res. 2023 Sep 5;11(1):78. [Content Brief]
[3]. Gordon SL, et al. Differential regulation of human tyrosine hydroxylase isoforms 1 and 2 in situ: Isoform 2 is not phosphorylated at Ser35. Biochim Biophys Acta. 2009 Dec;1793(12):1860-7. [Content Brief]
[4]. Bao L, et al. The S100A6 calcium-binding protein regulates endothelial cell-cycle progression and senescence. FEBS J. 2012 Dec;279(24):4576-88. [Content Brief]
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