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alpha 1 Antitrypsin Antibody (YA3592)

(Synonyms: PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT)
Customer Review

Based on 1 Customer Validation

alpha 1 Antitrypsin Antibody (YA3592) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to alpha 1 Antitrypsin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • alpha 1 Antitrypsin Antibody (YA3592) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

alpha 1 Antitrypsin Antibody (YA3592) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to alpha 1 Antitrypsin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Immunogen

Purified recombinant fragment of human SERPINA1 (AA: 23-237) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
    Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
    Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
    Immunohistochemical analysis of paraffin-embedded human Liver cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
    Immunohistochemical analysis of paraffin-embedded human Liver cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    alpha 1 Antitrypsin is an Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin; Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE)

  • Subcellular Localization

    Secreted; Endoplasmic reticulum; Secreted, extracellular space, extracellular matrix

  • Expression


    Tissue_specificity:It is ubiquitous. It is expressed in both leukocytes and plasma.

  • Isoforms & Post-Translational Modification

    P01009 has 3 isomers: P01009-1: 46737 Da (predicted); P01009-2: 40263 Da (predicted); P01009-3: 34755 Da (predicted).
    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant;Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418;(Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases

  • Subunit

    Interacts with CELA2A (PubMed:31358993). Interacts with ERGIC3 and LMAN1/ERGIC53 (PubMed:31142615). Interacts with PRSS1/trypsin (PubMed:11057674). Interacts with PRSS1/Trypsin (PubMed:11057674). The variants S and Z interact with CANX and PDIA3 (PubMed:23826168)

  • SwissProt ID

    P01009

  • Gene ID
    5265 [NCBI]
  • Synonyms

    PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT

alpha 1 Antitrypsin Antibody (YA3592) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • alpha 1 Antitrypsin Antibody (YA3592) Product Image
    1/16
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • alpha 1 Antitrypsin Antibody (YA3592) Product Image
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA3592)
  • alpha 1 Antitrypsin Antibody (YA3592) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • alpha 1 Antitrypsin Antibody (YA3592) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • alpha 1 Antitrypsin Antibody (YA3592) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • alpha 1 Antitrypsin Antibody (YA3592) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • alpha 1 Antitrypsin Antibody (YA3592) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Liver cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • alpha 1 Antitrypsin Antibody (YA3592) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Liver cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83895, 1:2000 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • alpha 1 Antitrypsin Antibody (YA3592) Product Image IHC-P
  • alpha 1 Antitrypsin Antibody (YA3592) Product Image IHC-P
  • alpha 1 Antitrypsin Antibody (YA3592) Product Image IHC-P
  • alpha 1 Antitrypsin Antibody (YA3592) Product Image IHC-P
  • alpha 1 Antitrypsin Antibody (YA3592) Product Image IHC-P
  • alpha 1 Antitrypsin Antibody (YA3592) Product Image IHC-P