Wnt5a Antibody (YA4002)
(Synonyms: hWNT5A)Wnt5a Antibody (YA4002) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Wnt5a.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, ICC/IF, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:1000 | 1:10000 |
Product Details
Wnt5a Antibody (YA4002) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Wnt5a.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 45 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 42 kDa
Purified recombinant fragment of WNT5A aa 75-380.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Wnt5a is a Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes[1][2].
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Subcellular Localization
Secreted, extracellular space, extracellular matrix; Secreted
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Expression
Tissue_specificity:This gene is expressed at elevated levels in differentiated thyroid carcinomas compared to normal thyroid tissue and undifferentiated thyroid tumors with low or undetectable expression levels. The gene is expressed in thyroid cells but not detected in stromal cells (protein level) (PubMed: 15735754) . It has also been detected in the hearts and lungs of newborns (PubMed: 8288227) . -
Isoforms & Post-Translational Modification
P41221 has 2 isomers: P41221-1: 42339 Da (predicted); P41221-2: 40887 Da (predicted).
Glycosylation is necessary for secretion but not for activity;Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition;Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT5A -
Subunit
Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720). The complex with AFM may represent the physiological form in body fluids (PubMed:26902720). Homooligomer; disulfide-linked, leading to inactivation (in vitro). Interacts with PORCN. Interacts with WLS (By similarity). Interacts with glypican GCP3 (PubMed:14610063). Interacts with PKD1 (via extracellular domain) (PubMed:27214281). Interacts with TMEM67 (PubMed:34731008)
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SwissProt ID
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Synonyms
hWNT5A
Documentation
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