2. Anti-infection
  3. CMV
  4. AX-7396

AX-7396 (RGB-315389) is a pUL97 protein kinase inhibitor with an IC50 of 0.35 μM against HCMV pUL97 kinase. AX-7396 inhibits the replication of wild-type and agent-resistant HCMV, GPCMV, MCMV and RCMV in vitro, with the strongest potency against MCMV (IC50 1.6 μM). AX-7396 shows no significant antiviral activity in a mouse cytomegalovirus infection model. AX-7396 can be used in studies related to cytomegalovirus infection.

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AX-7396

AX-7396 Chemical Structure

CAS No. : 824431-12-1

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Description

AX-7396 (RGB-315389) is a pUL97 protein kinase inhibitor with an IC50 of 0.35 μM against HCMV pUL97 kinase. AX-7396 inhibits the replication of wild-type and agent-resistant HCMV, GPCMV, MCMV and RCMV in vitro, with the strongest potency against MCMV (IC50 1.6 μM). AX-7396 shows no significant antiviral activity in a mouse cytomegalovirus infection model. AX-7396 can be used in studies related to cytomegalovirus infection[1][2].

In Vitro

Ax-7396 potently inhibits HCMV pUL97 kinase activity in vitro with an IC50 of 0.35 μM, and also targets specific cellular kinases including EGFR and IGF-1R[2].
Ax-7396 inhibits the replication of wild-type and drug-resistant HCMV, GPCMV, MCMV and RCMV in vitro, with IC50 values in the low micromolar range, and exhibits the strongest potency against MCMV (IC50 1.6 μM)[2].
Ax-7396 (0.1-90 μM; 3-7 days) exhibits low cytotoxicity toward primary mouse fibroblasts at concentrations that exert inhibitory effects on cytomegalovirus (CMV)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AX-7396 Related Antibodies

Cell Cytotoxicity Assay[2]

Cell Line: primary human foreskin fibroblasts (HFFs), primary human IMR90 cells, primary mouse embryonic fibroblasts (MEFs)
Concentration: 0.1-90 μM
Incubation Time: 3, 5, or 7 days (depending on assay)
Result: Showed no cytotoxicity in HFFs at concentrations up to 30 μM after 5 or 7 days of incubation.
Showed no notable cytotoxicity in MEFs at concentrations below 90 μM after 5 days of incubation.
Showed no significant reduction in cell viability in IMR90 cells at concentrations between 0.1 μM and 10 μM after 3 days of incubation.
Had CC50 values of 92.4 μM for HFFs (7 days) and 90.1 μM for MEFs (5 days), resulting in antiviral indices of 15.2 for HFF/HCMV and 56.3 for MEF/MCMV.
In Vivo

Ax7396 (50 mg/kg; i.p.; on days -1, 1, 4, and 7 relative to infection) shows no significant antiviral activity against MCMV in immunocompetent Balb/c mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Balb/c (6-8-week-old female, mean weight ~18 g, immunocompetent, inoculated i.p. with 5×105 PFU mouse cytomegalovirus strain Smith)[2]
Dosage: 50 mg/kg
Administration: i.p.; on days -1, 1, 4, and 7 relative to infection
Result: Resulted in a slight, non-significant reduction of infectious viral titers in salivary glands compared to placebo-treated controls.
Showed no significant inhibition of viral genomic loads in salivary glands, spleens, livers, or lungs relative to placebo.
Molecular Weight

304.17

Formula

C15H11Cl2N3
CAS No.
SMILES

ClC1=CC=C(NC2=C3C=C(C)C=CC3=NC=N2)C=C1Cl
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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AX-7396 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AX-7396824431-12-1RGB-315389AX7396AX 7396RGB315389RGB 315389CMVCytomegaloviruspUL97 protein kinasemouse cytomegalovirus infection modelEGFRhuman cytomegalovirusmouse fibroblastsM97 kinaseanimal cytomegalovirusIGF-1Rprimary human fibroblastshuman pUL97 kinaseInhibitorinhibitorinhibit

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