Yoda 1
Based on 102 publication(s) in Google Scholar
Yoda 1 is a potent and selective Piezo1 agonist. Yoda 1 activates purified Piezo1 channels. Yoda 1 potently inhibits macropinocytosis induced by epidermal growth factor (EGF). Yoda 1 enhances Ca2+ influx followed by activation of the calcium-activated potassium channel KCa3.1 and inhibition of Rac1 activation.
For research use only. We do not sell to patients.
- Purity: 99.97%
- CAS No.: 448947-81-7
- Formula: C13H8Cl2N4S2
- Molecular Weight:355.27
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Yoda 1
More- Signal Transduct Target Ther. 2025 Oct 28;10(1):357. [Abstract]
- Nat Immunol. 2026 May;27(5):949-960. [Abstract]
- Cell Res. 2026 Jan 9. [Abstract]
- Cancer Commun (Lond). 2022 Nov;42(11):1162-1184. [Abstract]
- Bioact Mater. 2025 Nov 20:57:531-550. [Abstract]
- ACS Nano. 2025 Dec 16;19(49):41746-41764. [Abstract]
- Nat Commun. 2025 Dec 16;17(1):272. [Abstract]
- Nat Commun. 2025 Jul 25;16(1):6868. [Abstract]
- Bone Res. 2026 May 28;14(1):59. [Abstract]
- Bone Res. 2025 Sep 24;13(1):79. [Abstract]
- Adv Sci (Weinh). 2026 Mar 4:e22936. [Abstract]
- Adv Sci (Weinh). 2025 Nov 10:e10991. [Abstract]
- Adv Sci (Weinh). 2025 Jul 26:e02052. [Abstract]
- Adv Sci (Weinh). 2024 Sep 11:e2409081. [Abstract]
- Adv Sci (Weinh). 2023 Nov;10(33):e2303369. [Abstract]
- J Adv Res. 2025 Oct 26:S2090-1232(25)00836-7. [Abstract]
- J Adv Res. 2024 Aug:62:105-117. [Abstract]
- Exp Mol Med. 2026 Feb;58(1):143-160. [Abstract]
- Sci Adv. 2026 Jan 16;12(3):eaea0998. [Abstract]
- Cell Discov. 2024 Nov 12;10(1):114. [Abstract]
- Sci Adv. 2024 Jun 7;10(23):eadj3289. [Abstract]
- Redox Biol. 2024 Sep 7:76:103346. [Abstract]
- Hum Reprod Open. 2026 Feb 17;2026(2):hoag013. [Abstract]
- Research (Wash D C). 2025 Jun 3:8:0718. [Abstract]
- Cardiovasc Diabetol. 2025 Mar 20;24(1):127. [Abstract]
- Mater Today Bio. 2026 Mar 3:37:102985. [Abstract]
- Mater Today Bio. 2025 Dec 3:36:102620. [Abstract]
- Mater Today Bio. 2025 Oct 21:35:102446. [Abstract]
- Mater Today Bio. 2025 Mar 8:31:101645. [Abstract]
- Cancer Lett. 2024 Feb 1:582:216597. [Abstract]
- Int J Biol Sci. 2026 Jan 15;22(4):1834-1851. [Abstract]
- Genes Dis. 2024 Sep 15;12(2):101434. [Abstract]
- J Cachexia Sarcopenia Muscle. 2025 Aug;16(4):e70004. [Abstract]
- Cell Commun Signal. 2025 Jun 13;23(1):280. [Abstract]
- Acta Pharmacol Sin. 2025 Jul 9. [Abstract]
- Small Sci. 2024 Jun 30;4(9):2400061. [Abstract]
- ACS Appl Mater Interfaces. 2025 Oct 15;17(41):56861-56876. [Abstract]
- Regen Biomater. 2025 Jun 16:12:rbaf056. [Abstract]
- Br J Pharmacol. 2025 May;182(9):1957-1974. [Abstract]
- J Transl Med. 2024 Apr 4;22(1):332. [Abstract]
- Biomed Pharmacother. 2023 Oct:166:115343. [Abstract]
- Cell Chem Biol. 2026 Mar 19;33(3):307-320.e4. [Abstract]
- Aging Cell. 2024 Nov 28:e14440. [Abstract]
- Cell Rep. 2025 Mar 12;44(3):115400. [Abstract]
- Cell Rep. 2025 Jan 28;44(1):115136. [Abstract]
- Clin Transl Med. 2025 Dec;15(12):e70509. [Abstract]
- Mol Med. 2025 Apr 22;31(1):147. [Abstract]
- Mol Med. 2025 Mar 8;31(1):90. [Abstract]
- Elife. 2022 Aug 22;11:e79957. [Abstract]
- J Agric Food Chem. 2025 Jul 23;73(29):18153-18164. [Abstract]
- JCI Insight. 2022 Apr 8;7(7):e152330. [Abstract]
- J Invest Dermatol. 2025 Mar 5:S0022-202X(25)00303-3. [Abstract]
- Biochem Pharmacol. 2025 Aug 29;242(Pt 1):117293. [Abstract]
- Cell Prolif. 2024 Aug;57(8):e13640. [Abstract]
- ACS Biomater Sci Eng. 2023 Aug 14;9(8):4735-4746. [Abstract]
- J Ethnopharmacol. 2024 Jul 30:335:118639. [Abstract]
- Life Sci. 2026 Mar 1:388:124195. [Abstract]
- Life Sci. 2025 May 15:369:123554. [Abstract]
- Life Sci. 2024 Jan 1:336:122326. [Abstract]
- CNS Neurosci Ther. 2024 Feb;30(2):e14365. [Abstract]
- Int J Mol Sci. 2023 Feb 16;24(4):4022. [Abstract]
- Biomolecules. 2024 Oct 16;14(10):1311. [Abstract]
- Int Immunopharmacol. 2025 Jul 26:163:115284. [Abstract]
- Eur J Pharmacol. 2024 Sep 3:176954. [Abstract]
- Neuropharmacology. 2024 Jun 15:251:109896. [Abstract]
- ACS Omega. 2025 May 16;10(29):31368-31380. [Abstract]
- J Cell Mol Med. 2024 Dec;28(23):e70237. [Abstract]
- iScience. 2025 Oct 8;28(11):113688. [Abstract]
- Cell Calcium. 2025 Oct 14:132:103084. [Abstract]
- Cell Calcium. 2022 Jun;104:102571. [Abstract]
- JOR Spine. 2025 Nov 4;8(4):e70133. [Abstract]
- J Biomed Mater Res A. 2025 Jun;113(6):e37931. [Abstract]
- Sci Rep. 2025 May 8;15(1):15975. [Abstract]
- J Biol Chem. 2024 Jan;300(1):105526. [Abstract]
- Cardiovasc Toxicol. 2025 Jul 24. [Abstract]
- Am J Pathol. 2023 Sep;193(9):1208-1222. [Abstract]
- Front Med. 2025 Dec 1. [Abstract]
- Stem Cells Int. 2022 Jul 29:2022:6202842. [Abstract]
- Cancer Med. 2025 Jul;14(14):e71059. [Abstract]
- Front Biosci (Landmark Ed). 2025 Apr 30;30(5):27094. [Abstract]
- Hum Cell. 2024 Nov;37(6):1649-1662. [Abstract]
- Ren Fail. 2024 Dec;46(2):2415519. [Abstract]
- Arch Biochem Biophys. 2023 Oct 1:747:109755. [Abstract]
- Discov Oncol. 2025 Apr 30;16(1):644. [Abstract]
- Mol Pain. 2025 Dec 12:17448069251410754. [Abstract]
- Ultrasound Med Biol. 2025 Mar;51(3):494-507. [Abstract]
- J Orthop Res. 2023 Aug;41(8):1792-1802. [Abstract]
- BMC Cardiovasc Disord. 2022 Jun 24;22(1):287. [Abstract]
- Biochem Biophys Res Commun. 2023 Jan 1:638:140-146. [Abstract]
- Biol Pharm Bull. 2023;46(1):1-11. [Abstract]
- Curr Med Sci. 2024 Aug;44(4):718-725. [Abstract]
- bioRxiv. 2026 May 28.
- bioRxiv. 2026 Jan 13.
- bioRxiv. 2025 August 07.
- SSRN. 2025 Aug 13.
- bioRxiv. 2025 July 04.
- SSRN. 2025 Jul 24.
- Research Square Preprint. 2024 Apr 5.
- Research Square Print. December 8th, 2022.
- Research Square Print. November 17th, 2022
- bioRxiv. May 18, 2022.
- SSRN. 9 Feb 2022.
Customer Validation & Images
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Cell Imaging/Staining
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IF
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WB
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WB
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Cell Proliferation/Viability Assay
Biological Activity
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ERK1 |
ERK2 |
Yoda1 (0-6 μM, 5 min) induces the activation of both Akt and ERK1/2, which is not dependent on Piezo1[2].
Yoda1 (1.5 μM, 5 min) inhibits Rac1 activation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human coronary artery endothelial cells (HCAECs)[2], A431 cells[3]
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Concentration:0, 1.5, 3.0, and 6.0 μM
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Incubation Time:5 min
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Result:Induced the activation of both Akt and ERK1/2, and increased the phosphorylation levels of both Akt and ERK1/2 in a dose-dependent manner. Inhibited EGF-induced increase in the amount of Rac1-GTP, and inhibited Rac1 activation
Chemical Information
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CAS No. 448947-81-7
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Appearance Solid
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Molecular Weight 355.27
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Formula C13H8Cl2N4S2
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Color White to light yellow
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SMILES
ClC1=C(CSC2=NN=C(C3=NC=CN=C3)S2)C(Cl)=CC=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (102)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Piezo1 activation suppresses bone marrow adipogenesis to prevent osteoporosis by inhibiting a mechanoinflammatory autocrine loop. [Abstract]2025 Oct 28;10(1):357. PMID: 41145414 -
Nat Immunol
Lipid asymmetry disruption by XKR8 orchestrates neutrophil extracellular trap formation and inhibits fungal infection. [Abstract]2026 May;27(5):949-960. PMID: 41781710 -
Cell Res
Transient mechanical activation of the Piezo1 channel facilitates ex vivo expansion of hematopoietic stem cells. [Abstract]2026 Jan 9. PMID: 41507440 -
Cancer Commun (Lond)
Activation of Piezo1 contributes to matrix stiffness-induced angiogenesis in hepatocellular carcinoma. [Abstract]2022 Nov;42(11):1162-1184. PMID: 36181398 -
Bioact Mater
Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1. [Abstract]2025 Nov 20:57:531-550. PMID: 41340637 -
ACS Nano
Tumor Mechanobiology-Inspired Nanomechanics: Piezo1 Mediated Tumor-Associated Macrophages Reprogramming and Exosome-Driven Immune Amplification. [Abstract]2025 Dec 16;19(49):41746-41764. PMID: 41332254 -
Nat Commun
Polypeptide sono-adjuvant for ultrasound-activatable regulation of innate immunity and cancer vaccination therapy. [Abstract]2025 Dec 16;17(1):272. PMID: 41402324 -
Nat Commun
Spatiotemporal H2O2 flashes coordinate actin cytoskeletal remodeling and regulate cell migration and wound healing. [Abstract]2025 Jul 25;16(1):6868. PMID: 40715145 -
Bone Res
KDM6B safeguards mineralized tissue homeostasis from mechanical stress through epigenetic control of PIEZO1-mediated mechanotransduction in the mouse incisor. [Abstract]2026 May 28;14(1):59. PMID: 42209460 -
Bone Res
Mechanism of Piezo1 regulating chondrocyte mitochondrial function and promoting fracture healing through β-catenin/LARS2 signaling pathway. [Abstract]2025 Sep 24;13(1):79. PMID: 40993116 -
Adv Sci (Weinh)
Piezo1 Channel Mediates Mechanically Programmable Drug Delivery to Potentiate Intravesical Chemotherapy. [Abstract]2026 Mar 4:e22936. PMID: 41782384 -
Adv Sci (Weinh)
Piezo1 Upregulation in Monocyte-Derived Macrophages Impairs Post-Myocardial Infarction Cardiac Repair via Defective Efferocytosis and Enhanced Ferroptosis. [Abstract]2025 Nov 10:e10991. PMID: 41214880 -
Adv Sci (Weinh)
PIEZO1-GPX4 Axis Mediates Mechanical Stress-Induced Vertebral Growth Plate Dysplasia via Ferroptosis Activation. [Abstract]2025 Jul 26:e02052. PMID: 40714837 -
Adv Sci (Weinh)
Enhanced Tumor-Targeted Delivery of Arginine-Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis. [Abstract]2024 Sep 11:e2409081. PMID: 39258781
Yoda 1 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Sep 11:e2409081. [Abstract]
Expression levels of Piezo1/integrin β1/YAP signal in T24 cells exposed to Yoda1 or GsMTx4.
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Adv Sci (Weinh)
Actomyosin Activity and Piezo1 Activity Synergistically Drive Urinary System Fibroblast Activation. [Abstract]2023 Nov;10(33):e2303369. PMID: 37867255 -
J Adv Res
Matrix stiffness drives squamous cell carcinoma progression via a Piezo1-mediated mechanotransduction feedback loop. [Abstract]2025 Oct 26:S2090-1232(25)00836-7. PMID: 41151627 -
J Adv Res
Piezo1 activation accelerates osteoarthritis progression and the targeted therapy effect of artemisinin. [Abstract]2024 Aug:62:105-117. PMID: 37758057
Yoda 1 purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Aug:62:105-117. [Abstract]
Western blot of p-PI3K, PI3K, p-AKT and AKT in OA HACs treated with si-PIEZO1 for 48 h.
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Exp Mol Med
Piezo1 activation in endothelial cells aggravates microvascular ischemia-reperfusion injury in limbs by enhancing ferroptosis. [Abstract]2026 Feb;58(1):143-160. PMID: 41507309 -
Sci Adv
Cell surface engineering with a pseudofibrotic ECM reprograms the antifibrotic activity of mesenchymal stromal cells. [Abstract]2026 Jan 16;12(3):eaea0998. PMID: 41544160 -
Cell Discov
Cancer cells sense solid stress to enhance metastasis by CKAP4 phase separation-mediated microtubule branching. [Abstract]2024 Nov 12;10(1):114. PMID: 39528501 -
Sci Adv
Stiffness sensing via Piezo1 enhances macrophage efferocytosis and promotes the resolution of liver fibrosis. [Abstract]2024 Jun 7;10(23):eadj3289. PMID: 38838160 -
Redox Biol
LOX-mediated ECM mechanical stress induces Piezo1 activation in hypoxic-ischemic brain damage and identification of novel inhibitor of LOX. [Abstract]2024 Sep 7:76:103346. PMID: 39260063
Yoda 1 purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Sep 7:76:103346. [Abstract]
Protein levels of GPX4 and LOX in the control, OGD/R-induced, BAPN-treated OGD/R, and BAPN combined with Yoda1 (5 nM) treatment groups of HT22 cells.
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Hum Reprod Open
Impaired PIEZO1 function drives uterine hypercontractility in adenomyosis-associated dysmenorrhea. [Abstract]2026 Feb 17;2026(2):hoag013. PMID: 41836349 -
Research (Wash D C)
Piezo1-Mediated Ferroptosis Delays Wound Healing in Aging Mice by Regulating the Transcriptional Activity of SLC7A11 through Activating Transcription Factor 3. [Abstract]2025 Jun 3:8:0718. PMID: 40463502 -
Cardiovasc Diabetol
Piezo1 deletion mitigates diabetic cardiomyopathy by maintaining mitochondrial dynamics via ERK/Drp1 pathway. [Abstract]2025 Mar 20;24(1):127. PMID: 40114130 -
Mater Today Bio
Age-mimicking hydrogel stiffness recapitulates the mechanical niche of the hippocampus to regulate neural stem cell senescence. [Abstract]2026 Mar 3:37:102985. PMID: 41852885 -
Mater Today Bio
Piezo1 mediated scaffold-free rapid generation of self-mineralized bone organoids via activating Wnt signaling. [Abstract]2025 Dec 3:36:102620. PMID: 41476751
Yoda 1 purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Dec 3:36:102620. [Abstract]
Pre-treatment with Dooku1 slowed the rate of spheroid formation.
Yoda 1 purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Dec 3:36:102620. [Abstract]
IF staining confirmed the on-target activity of Dooku1, as it significantly suppressed the Yoda1 (0.5 μM)-induced upregulation of Piezo1 itself
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Mater Today Bio
Magnetically guided neurite outgrowth modulated by PIEZO1 in hiPSC-derived retinal ganglion cells. [Abstract]2025 Oct 21:35:102446. PMID: 41209706 -
Mater Today Bio
Uniform and controllable surface nano-structure on polyetheretherketone implants can regulate mechanical property to enhance soft tissue integration through Piezo1/TGF-β1 signaling axis. [Abstract]2025 Mar 8:31:101645. PMID: 40151615 -
Cancer Lett
2024 Feb 1:582:216597. PMID: 38145655 -
Int J Biol Sci
Abnormal mechanical load aggravates subchondral bone remodeling and uneven tibial plateau settlement in knee osteoarthritis via activation of osteoblast Piezo1-Ca²⁺-JAK2/STAT3 signaling. [Abstract]2026 Jan 15;22(4):1834-1851. PMID: 41694589 -
Genes Dis
Heavy mechanical force decelerates orthodontic tooth movement via Piezo1-induced mitochondrial calcium down-regulation. [Abstract]2024 Sep 15;12(2):101434. PMID: 39759122 -
J Cachexia Sarcopenia Muscle
Unravelling the Role of PIEZO1 in Adipogenesis of Fibrogenic/Adipogenic Progenitors for Muscle Fat Infiltration After Rotator Cuff Tear. [Abstract]2025 Aug;16(4):e70004. PMID: 40726363 -
Cell Commun Signal
Activation of mechanosensitive ion channel Piezo1 linking metabolic reprogramming and pro-inflammatory responses in hepatocellular carcinoma. [Abstract]2025 Jun 13;23(1):280. PMID: 40514668 -
Acta Pharmacol Sin
SERCA2 regulates Piezo1 channel activation and contributes to the cardiac function and baroreflex in mice. [Abstract]2025 Jul 9. PMID: 40629025 -
Small Sci
A Novel Piezo1 Agonist Promoting Mesenchymal Stem Cell Proliferation and Osteogenesis to Attenuate Disuse Osteoporosis. [Abstract]2024 Jun 30;4(9):2400061. PMID: 40212079
Yoda 1 purchased from MedChemExpress. Usage Cited in: Small Sci. 2024 Jun 30;4(9):2400061. [Abstract]
Cell viability curve of Yoda1 (0.5-16 μM; MSCs)
Yoda 1 purchased from MedChemExpress. Usage Cited in: Small Sci. 2024 Jun 30;4(9):2400061. [Abstract]
Western blot images of CaMKII, p-CaMKII, ERK, and p-Eek in rMSCs after 0–60 min 5 μm Yoda1 treatment.
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ACS Appl Mater Interfaces
Surficial Pore Structure on Polyetheretherketone Implants Regulates Mechanical Properties to Promote Macrophage M2 Polarization. [Abstract]2025 Oct 15;17(41):56861-56876. PMID: 41037664 -
Regen Biomater
Matrix stiffness boosts PDAC chemoresistance via SCD1-dependent lipid metabolic reprogramming. [Abstract]2025 Jun 16:12:rbaf056. PMID: 40741620 -
Br J Pharmacol
Involvement of Piezo 1 in inhibition of shear-induced platelet activation and arterial thrombosis by ginsenoside Rb1. [Abstract]2025 May;182(9):1957-1974. PMID: 39894463 -
J Transl Med
Intestinal Piezo1 aggravates intestinal barrier dysfunction during sepsis by mediating Ca2+ influx. [Abstract]2024 Apr 4;22(1):332. PMID: 38575957 -
Biomed Pharmacother
Barasertib impedes chondrocyte senescence and alleviates osteoarthritis by mitigating the destabilization of heterochromatin induced by AURKB. [Abstract]2023 Oct:166:115343. PMID: 37634474 -
Cell Chem Biol
2026 Mar 19;33(3):307-320.e4. PMID: 41650963 -
Aging Cell
Piezo1 exacerbates inflammation-induced cartilaginous endplate degeneration by activating mitochondrial fission via the Ca2+/CaMKII/Drp1 axis. [Abstract]2024 Nov 28:e14440. PMID: 39610146 -
Cell Rep
Piezo1 regulates colon stem cells to maintain epithelial homeostasis through SCD1-Wnt-β-catenin and programming fatty acid metabolism. [Abstract]2025 Mar 12;44(3):115400. PMID: 40080500 -
Cell Rep
2025 Jan 28;44(1):115136. PMID: 39932192 -
Clin Transl Med
Metabolic enzyme PFKFB3 mediates matrix stiffness-potentiated tumour growth and radiotherapeutic resistance in HCC. [Abstract]2025 Dec;15(12):e70509. PMID: 41292194 -
Mol Med
Endothelial Piezo1 stimulates angiogenesis to offer protection against intestinal ischemia-reperfusion injury in mice. [Abstract]2025 Apr 22;31(1):147. PMID: 40263994 -
Mol Med
Piezo1 promotes intervertebral disc degeneration through the Ca2+/F-actin/Yap signaling axis. [Abstract]2025 Mar 8;31(1):90. PMID: 40057686 -
Elife
2022 Aug 22;11:e79957. PMID: 35993548 -
J Agric Food Chem
2025 Jul 23;73(29):18153-18164. PMID: 40662381 -
JCI Insight
2022 Apr 8;7(7):e152330. PMID: 35230979 -
J Invest Dermatol
Endothelial Piezo1 Mediates Barrier Dysfunction and NLRP3 Inflammasomes Activation in Psoriasis. [Abstract]2025 Mar 5:S0022-202X(25)00303-3. PMID: 40054572 -
Biochem Pharmacol
Piezo1 promotes colitis progression by modulating the Nrf2/NF-κB/NLRP3 signaling pathway. [Abstract]2025 Aug 29;242(Pt 1):117293. PMID: 40886854 -
Cell Prolif
2024 Aug;57(8):e13640. PMID: 38556840 -
ACS Biomater Sci Eng
Three-Dimensional Matrix Stiffness Activates the Piezo1-AMPK-Autophagy Axis to Regulate the Cellular Osteogenic Differentiation. [Abstract]2023 Aug 14;9(8):4735-4746. PMID: 37428711 -
J Ethnopharmacol
Effects of Panax notoginseng saponins on alleviating low shear induced endothelial inflammation and thrombosis via Piezo1 signalling. [Abstract]2024 Jul 30:335:118639. PMID: 39084271 -
Life Sci
Compressive stress induces cartilage endplate degeneration through the Piezo1/YAP-TEAD/NLRP3 axis. [Abstract]2026 Mar 1:388:124195. PMID: 41506536 -
Life Sci
Trimethylamine N-oxide induces cardiac diastolic dysfunction by down-regulating Piezo1 in mice with heart failure with preserved ejection fraction. [Abstract]2025 May 15:369:123554. PMID: 40074144 -
Life Sci
Regulatory role of Piezo1 channel in endothelium-dependent hyperpolarization-mediated vasorelaxation of small resistance vessels and its anti-inflammatory action. [Abstract]2024 Jan 1:336:122326. PMID: 38056769 -
CNS Neurosci Ther
Enriched environment ameliorates fear memory impairments induced by sleep deprivation via inhibiting PIEZO1/calpain/autophagy signaling pathway in the basal forebrain. [Abstract]2024 Feb;30(2):e14365. PMID: 37485782 -
Int J Mol Sci
Gsmtx4 Alleviated Osteoarthritis through Piezo1/Calcineurin/NFAT1 Signaling Axis under Excessive Mechanical Strain. [Abstract]2023 Feb 16;24(4):4022. PMID: 36835440 -
Biomolecules
Caution for Multidrug Therapy: Significant Baroreflex Afferent Neuroexcitation Coordinated by Multi-Channels/Pumps Under the Threshold Concentration of Yoda1 and Dobutamine Combination. [Abstract]2024 Oct 16;14(10):1311. PMID: 39456244 -
Int Immunopharmacol
Piezo1 participates in sevoflurane-induced developmental neurotoxicity via neuroinflammation and synaptic damage in the hippocampus. [Abstract]2025 Jul 26:163:115284. PMID: 40716188 -
Eur J Pharmacol
Activation of Piezo1 channels enhances spontaneous contractions of isolated human bladder strips via acetylcholine release from the mucosa. [Abstract]2024 Sep 3:176954. PMID: 39237075 -
Neuropharmacology
Roles of mechanosensitive ion channel PIEZO1 in the pathogenesis of brain injury after experimental intracerebral hemorrhage. [Abstract]2024 Jun 15:251:109896. PMID: 38490299 -
ACS Omega
The Critical Role of Piezo1/CaMKII/β-Catenin Axis in Promoting Osteogenic Differentiation of ADSCs by Pressure Stimulation. [Abstract]2025 May 16;10(29):31368-31380. PMID: 40757337 -
J Cell Mol Med
Endothelial KDM5B Regulated by Piezo1 Contributes to Disturbed Flow Induced Atherosclerotic Plaque Formation. [Abstract]2024 Dec;28(23):e70237. PMID: 39643939 -
iScience
Agonism of PIEZO1 prevents aggravated periodontitis with traumatic occlusion via MAPK signaling pathway. [Abstract]2025 Oct 8;28(11):113688. PMID: 41234768 -
Cell Calcium
Endothelial PIEZO1 activation impairs osteogenesis via GREM1-mediated inhibition of BMP signaling. [Abstract]2025 Oct 14:132:103084. PMID: 41135331 -
Cell Calcium
Mechanosensitive Piezo1 channel in rat epididymal epithelial cells promotes transepithelial K+ secretion. [Abstract]2022 Jun;104:102571. PMID: 35314382 -
JOR Spine
PIEZO1-Primary Cilia Axis Mediates Compressive Stress-Induced Growth Plate Degeneration and Ossification in Adolescent Idiopathic Scoliosis. [Abstract]2025 Nov 4;8(4):e70133. PMID: 41194970 -
J Biomed Mater Res A
Piezo1 Is an Early Mediator During Topography-Stimulated Osteogenic Differentiation of Bone Mesenchymal Stem Cells. [Abstract]2025 Jun;113(6):e37931. PMID: 40405805 -
Sci Rep
Piezo1 activation protects against sepsis-induced myocardial dysfunction in a pilot study. [Abstract]2025 May 8;15(1):15975. PMID: 40341084 -
J Biol Chem
AKT Signaling Modulates Latent Viral Reservoir Viability in HIV-1-Infected Blood-Brain Barrier Pericytes. [Abstract]2024 Jan;300(1):105526. PMID: 38043797 -
Cardiovasc Toxicol
Yoda1/Piezo1 Alleviates Lipopolysaccharide-Induced Cardiac Injury via AMPK-Mediated Mitophagy. [Abstract]2025 Jul 24. PMID: 40705239 -
Am J Pathol
Fibrotic matrix induces mesenchymal transformation of epithelial cells in oral submucous fibrosis. [Abstract]2023 Sep;193(9):1208-1222. PMID: 37328100 -
Front Med
Sub-threshold depolarization differentially regulated by multiple ion channels plays a key role in initiation of baroreflex afferent neurotransmission evidenced from baroreceptor terminals to its cell bodies. [Abstract]2025 Dec 1. PMID: 41324839 -
Stem Cells Int
Low-Intensity Pulsed Ultrasound Enhanced Adipose-Derived Stem Cell-Mediated Angiogenesis in the Treatment of Diabetic Erectile Dysfunction through the Piezo-ERK-VEGF Axis. [Abstract]2022 Jul 29:2022:6202842. PMID: 35935181 -
Cancer Med
Piezo1/ITGB1 Synergizes With Ca2+/YAP Signaling to Propel Bladder Carcinoma Progression via a Stiffness-Dependent Positive Feedback Loop. [Abstract]2025 Jul;14(14):e71059. PMID: 40667648 -
Front Biosci (Landmark Ed)
Investigation Into the Pathogenesis of Type 2 Cardiorenal Syndrome via the ROS-TRPM2 Signaling Axis. [Abstract]2025 Apr 30;30(5):27094. PMID: 40464493 -
Hum Cell
Cyclic tensile stress promotes osteogenic differentiation via upregulation of Piezo1 in human dental follicle stem cells. [Abstract]2024 Nov;37(6):1649-1662. PMID: 39190266 -
Ren Fail
Piezo1 facilitates the initiation and progression of renal fibrosis by mediating cell apoptosis and mitochondrial dysfunction. [Abstract]2024 Dec;46(2):2415519. PMID: 39496543 -
Arch Biochem Biophys
Different stimuli induce endothelial dysfunction and promote atherosclerosis through the Piezo1/YAP signaling axis. [Abstract]2023 Oct 1:747:109755. PMID: 37714252 -
Discov Oncol
The increased matrix stiffness caused by LOXL2 activates Piezo1 channels to promote the migration and invasion of cervical cancer cells. [Abstract]2025 Apr 30;16(1):644. PMID: 40304808 -
Mol Pain
EXPRESS: Piezo1-mediated neuroexcitation via collaboration with KCa1.1 and Nav1.9 currents in myelinated Ah-type of trigeminal ganglion neurons in rats: mechanistic insights with sex-specific effects. [Abstract]2025 Dec 12:17448069251410754. PMID: 41384612 -
Ultrasound Med Biol
2025 Mar;51(3):494-507. PMID: 39632209 -
J Orthop Res
Compressive stress induces spinal vertebral growth plate chondrocytes apoptosis via Piezo1. [Abstract]2023 Aug;41(8):1792-1802. PMID: 36722421 -
BMC Cardiovasc Disord
Agonist-induced Piezo1 activation promote mitochondrial-dependent apoptosis in vascular smooth muscle cells. [Abstract]2022 Jun 24;22(1):287. PMID: 35751027 -
Biochem Biophys Res Commun
2023 Jan 1:638:140-146. PMID: 36455360 -
Biol Pharm Bull
Chemical Activation of Piezo1 Alters Biomechanical Behaviors toward Relaxation of Cultured Airway Smooth Muscle Cells. [Abstract]2023;46(1):1-11. PMID: 36596517 -
Curr Med Sci
2024 Aug;44(4):718-725. PMID: 38926331 -
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Solvent & Solubility
DMSO : 15.62 mg/mL (43.97 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : 5 mg/mL (14.07 mM; Need ultrasonic)
Methanol : 2.5 mg/mL (7.04 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.56 mg/mL (4.39 mM); Clear solution
This protocol yields a clear solution of ≥ 1.56 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.6 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 0.62 mg/mL (1.75 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Syeda R, et al. Chemical activation of the mechanotransduction channel Piezo1. Elife. 2015 May 22;4. [Content Brief]
[2]. Dela Paz NG, et al. Yoda1-induced phosphorylation of Akt and ERK1/2 does not require Piezo1 activation. Biochem Biophys Res Commun. 2018 Feb 26;497(1):220-225. [Content Brief]
[3]. Kuriyama M, et al. Piezo1 activation using Yoda1 inhibits macropinocytosis in A431 human epidermoid carcinoma cells. Sci Rep. 2022 Apr 15;12(1):6322. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Methanol / Ethanol / DMSO | 1 mM | 2.8148 mL | 14.0738 mL | 28.1476 mL | 70.3690 mL |
| 5 mM | 0.5630 mL | 2.8148 mL | 5.6295 mL | 14.0738 mL | |
| Ethanol / DMSO | 10 mM | 0.2815 mL | 1.4074 mL | 2.8148 mL | 7.0369 mL |
| DMSO | 15 mM | 0.1877 mL | 0.9383 mL | 1.8765 mL | 4.6913 mL |
| 20 mM | 0.1407 mL | 0.7037 mL | 1.4074 mL | 3.5185 mL | |
| 25 mM | 0.1126 mL | 0.5630 mL | 1.1259 mL | 2.8148 mL | |
| 30 mM | 0.0938 mL | 0.4691 mL | 0.9383 mL | 2.3456 mL | |
| 40 mM | 0.0704 mL | 0.3518 mL | 0.7037 mL | 1.7592 mL |
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