Calotatug  (Synonyms: XMT-1519; XMT-1522 antibody)

HY-P990909 is an ERBB2-targeting IgG1κ type human antibody, the recommed isotype control is Human IgG1 kappa, Isotype Control (HY-P99001)^[1].

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Human

ERBB2/HER2/CD340

2064  [NCBI]

P04626

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

144.113 kDa

2254097-05-5

Liquid

Colorless to light yellow

[Calotatug]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  IgG1-kappa

• 
  Loaded Calotatug on ProA biosensor, can bind HER2/CD340 Protein, Human (HEK293, His, HY-P70254) with an affinity constant of 3.325E-08 M as determined in BLI assay.
• 
  Flow cytometric analysis of 1×10⁶ MCF-7 cells with Calotatug (HY-P990909, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Calotatug. IGMT database.