1240465-12-6
Chemical Structure
DBHA
- CAS No.: 1240465-12-6
- Formula:C28H30N2O5
- Molecular Weight:474.55
InChIKey: IAHPVRARRSGFRJ-SOFGYWHQSA-N
SMILES: O=C(C1=C2C=C(C=C1)OCCC/C=C/COC(C)=O)C=C(OC3=C4)C2=NC3=CC=C4N(CC)CC
Biological Activity: DBHA is a long-wavelength polarity-sensitive fluorescent probe based on Nile Red. Under the catalysis of Pd (OAc)2/TPPTS, DBHA site-selectively labels protein tyrosine residues via a π-allyl palladium complex intermediate. Utilizing the property that its maximum emission wavelength blue-shifts with decreasing local polarity (650 nm in aqueous solution → 584-608 nm in hydrophobic domains), it detects changes in local polarity and conformation of protein tyrosine domains under acid/thermal denaturation conditions. DBHA can be used in studies related to SOD1-associated oxidative stress neurodegenerative diseases[1][2].
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DBHA | DBHA is a long-wavelength polarity-sensitive fluorescent probe based on Nile Red. Under the catalysis of Pd (OAc)2/TPPTS, DBHA site-selectively labels protein tyrosine residues via a π-allyl palladium complex intermediate. Utilizing the property that its maximum emission wavelength blue-shifts with decreasing local polarity (650 nm in aqueous solution → 584-608 nm in hydrophobic domains), it detects changes in local polarity and conformation of protein tyrosine domains under acid/thermal denaturation conditions. DBHA can be used in studies related to SOD1-associated oxidative stress neurodegenerative diseases. | |||||||||||||||||||||
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References
- [1]. Chen S, et al. New approach for local structure analysis of the tyrosine domain in proteins by using a site-specific and polarity-sensitive fluorescent probe. Chembiochem : a European journal of chemical biology. 2009 May 04;10(7):1200-7. [Content Brief]
- [2]. Chang JW, et al. Chemoproteomic Profiling of Phosphoaspartate Modifications in Prokaryotes. Angewandte Chemie (International ed. in English). 2018 Nov 26;57(48):15712-15716.
