CDy11 

CDy11 is a fluorescent probe and amyloid-binding dye (λ_ex=590 nm; λ_em=612 nm), with a K_a of 29 μM for Pseudomonas aeruginosa Fap. CDy11 specifically recognizes amyloid fibrils in bacterial biofilms and exhibits significantly enhanced fluorescence upon binding to the target. CDy11 shows no staining effect on amyloid-deficient mutant strains, planktonic cells or protein monomers. CDy11 supports in vivo imaging of Pseudomonas aeruginosa biofilms in mouse implant and corneal infection models. CDy11 is widely used in studies of Staphylococcus aureus biofilm infections, dental caries, and Pseudomonas aeruginosa-associated implant and corneal infections^[1][2][3].

CDy11 (100 μM; 45 minutes) specifically detects amyloid fibril matrix components of Staphylococcus aureus GFP-SH1000 biofilm-coated silica microbeads, producing a strong red fluorescent signal that co-localizes with bacterial green fluorescence^[1].
CDy11 detects Pseudomonas aeruginosa biofilms with a limit of 8×10⁸ CFU/mL^[2].
CDy11 (1 μM; 1 h) co-localizes with Fap-specific antibody staining in Pseudomonas aeruginosa PAO1 biofilms, confirming specific binding to Fap amyloid^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CDy11 (5 μM; topical drop; single dose; 10-minute incubation followed by PBS wash) specifically detects P. aeruginosa biofilms in a mouse corneal infection model, with fluorescence signal peaking 10 minutes post-topical administration and colocalizing with biofilm-associated Fap amyloid^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

489.30

C₂₇H₂₃BF₃N₃O₂

2028316-07-4

CC(C=C1/C=C/C2=C(F)C=C(OC)C=C2)=C(N1[B-]3(F)F)C(C4=CC=C(NC(C)=O)C=C4)=C5[N+]3=CC=C5

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Sauvat L, et al. Biofilm-coated microbeads and the mouse ear skin: An innovative model for analysing anti-biofilm immune response in vivo. PLoS One. 2020;15(12):e0243500. Published 2020 Dec 4.

  [2]. Kim JY, et al. Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11. J Am Chem Soc. 2016;138(1):402-407.

  [3]. Barran-Berdon AL, et al. Enhanced purification coupled with biophysical analyses shows cross-β structure as a core building block for Streptococcus mutans functional amyloids. Sci Rep. 2020;10(1):5138. Published 2020 Mar 20.