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Art. -Nr.: HY-D0972

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Thioflavine S

Name: Thioflavine S
Brand: MedChemExpress
SKU: HY-D0972
Price: 66.00 USD
Availability: InStock
Rating: 5 (31 reviews)

(Synonyms: Thioflavin S; Direct Yellow 7)

31 Cited Publications

Kundenbewertung

Based on 31 publication(s) in Google Scholar

Thioflavine S is a fluorescent histochemical marker of dense core senile plaques. Thioflavine S can be used for Alzheimer's research.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr.: 1326-12-1

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Thioflavine S Chemical Structure

CAS. Nr.: 1326-12-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- Dyeing Example
Chemical Information
Publications (31)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Thioflavine S is a fluorescent histochemical marker of dense core senile plaques. Thioflavine S can be used for Alzheimer's research^[1].

In Vitro

Guidelines (The following is our recommended experimental protocol. This protocol is for reference only, and specific operations should be adjusted according to your actual needs).
Thioflavine S staining protocol
1.Deparaffinization and hydration of tissue sections: Place slides in holders and treat with the clearing agent xylene (paraffin solvent) and a series of graded EtOH as follows:
100% xylene - 5 min
100% xylene - 5 min
50%/50% xylene/100% EtOH - 3 min
100% EtOH - 3 min
95% EtOH - 3 min
70% EtOH - 3 min
50% EtOH - 3 min
Water - 2 x 3 min
Note: Change the solns after about 5 uses or when you see the soln is not cleanly running off the slides, indicating too much paraffin in them.
2.Incubate in filtered 1% aqueous Thioflavine-S for 8 minutes at room temperature (filter Thioflavin-S before each use). Note: protect thioflavin-S from light, and protect the stained slides from light as much as possible. Thioflavin-S stain should be stored at 4°C.
3.Wash 2x 3 min in 80% ethanol
4.Wash 3 min in 95% ethanol
5.Wash with 3 exchanges of distilled water
6.Coverslip in aqueous mounting media and allow slides to dry in the dark overnight.
7.Next day, seal coverslip with clear nail polish.
8.Analyze slides within the next few days-weeks because the staining will fade with time. Store the slides in the dark at 4°C.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dyeing Example

Chemical Information

CAS. Nr. 1326-12-1
Appearance Solid
Color Yellow to brown
SMILES

[Thioflavin S]
Synonyms

Thioflavin S; Direct Yellow 7
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (31)

All Histological Imaging/StainingIF

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Journal Impact Factor

Cell

Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2. [Abstract]

2026 Feb 5;189(3):853-871.e31. PMID: 41576952
Adv Mater

Engineered Extracellular Vesicles with SHP2 High Expression Promote Mitophagy for Alzheimer's Disease Treatment. [Abstract]

2022 Dec;34(49):e2207107. PMID: 36193769
Nat Aging

Allosteric activation of a cell-type-specific GPR120 inhibits amyloid pathology of Alzheimer's disease. [Abstract]

2025 Dec 19. PMID: 41419665

Thioflavine S purchased from MedChemExpress. Usage Cited in: Nat Aging. 2025 Dec 19. [Abstract]

Brain sections stained were stained with Thioflavin S (green; 500 μM). DAPI was used for nuclear staining (blue).
Autophagy

Upregulation of ISG15 induced by MAPT/tau accumulation represses autophagic flux by inhibiting HDAC6 activity: a vicious cycle in Alzheimer disease. [Abstract]

2025 Apr;21(4):807-826. PMID: 39635882
Nat Commun

Pharmacologic inhibition of PCBP2 biomolecular condensates relieves Alzheimer's disease. [Abstract]

2025 Nov 26;16(1):10514. PMID: 41298370

Thioflavine S purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 26;16(1):10514. [Abstract]

Representative immunofluorescence images showing that mCherry-PCBP2 condensates (red) do not substantially colocalize with the Thioflavin S (ThioS; 0.1% w/v) in SH-SY5Y-mCherry-PCBP2 cells. Arrows in green: amyloid plaques or aggresome, red: PCBP2 condensates.
Chem Eng J

2024 Sep 1.
EBioMedicine

KIBRA regulates amyloid β metabolism by controlling extracellular vesicles secretion. [Abstract]

2022 Apr;78:103980. PMID: 35367771

Thioflavine S purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2022 Apr;78:103980. [Abstract]

Thioflavin S (ThS; 500 µM; 8 min). Representative images and quantification analysis of ThS-stained insoluble Aβ plaques in whole brain of 3-5-month-old mice.
Mol Psychiatry

Dual recombinase-mediated intersectional genetics defines the functional heterogeneity of neural stem cells in adult hippocampus. [Abstract]

2025 Aug;30(8):3516-3532. PMID: 39994425
Basic Res Cardiol

Attenuation of myocardial ischemia-reperfusion injury in mice through CD80/86 deficiency: improved microvascular obstruction via reduced macrophage and T lymphocyte infiltration. [Abstract]

2025 Aug 1. PMID: 40748372
Basic Res Cardiol

Protective effect of HINT2 on mitochondrial function via repressing MCU complex activation attenuates cardiac microvascular ischemia-reperfusion injury. [Abstract]

2021 Dec 16;116(1):65. PMID: 34914018

Thioflavine S purchased from MedChemExpress. Usage Cited in: Basic Res Cardiol. 2021 Dec 16;116(1):65. [Abstract]

Thioflavin S (1 mL/kg; Tail vein injection). Endothelial-specific overexpression of HINT2 protected the cardiac microcirculation against I/R injury by modulating the MCU complex. Mice were transfected with AAV9-NC or AAV9-HINT2 and suffered from 45 min ischemia and 6 h reperfusion injury. Ru360 was injected intraperitoneally into AAV9-NC mice 1 h before I/R injury to inhibit the MCU complex. Spermine was injected intraperitoneally into AAV9-HINT2 mice 1 h before I/R injury to activate the MCU complex. No reflow area after reperfusion was detected by Thioflavin S staining and quantified.
Alzheimers Res Ther

Histone deacetylase inhibitors VPA and WT161 ameliorate the pathological features and cognitive impairments of the APP/PS1 Alzheimer's disease mouse model by regulating the expression of APP secretases. [Abstract]

2024 Jan 20;16(1):15. PMID: 38245771
Sens Actuators B Chem

2025 Oct 1.
Transl Psychiatry

2026 Jul 4. PMID: 42401549
J Ginseng Res

Ginsenoside Rg1 alleviates Aβ deposition by inhibiting NADPH oxidase 2 activation in APP/PS1 mice. [Abstract]

2021 Nov;45(6):665-675. PMID: 34764721

Thioflavine S purchased from MedChemExpress. Usage Cited in: J Ginseng Res. 2021 Nov;45(6):665-675. [Abstract]

The Aβ deposition in cortex and hippocamus (1% Thioflavin-S staining, yellow arrows indicate Aβ deposition).
Int Immunopharmacol

Stress level of glucocorticoid exacerbates neuronal damage and Aβ production through activating NLRP1 inflammasome in primary cultured hippocampal neurons of APP-PS1 mice. [Abstract]

2022 Sep:110:108972. PMID: 35777263
Mol Neurobiol

Microcystin-LR Exposure Damages Neurons by Inducing α-Syn Aggregation via MAPK4/GATA2/SNCA and PP2A/GRKs Pathways. [Abstract]

2025 May;62(5):6195-6211. PMID: 39738876
Front Cell Infect Microbiol

Curcumin-driven reprogramming of the gut microbiota and metabolome ameliorates motor deficits and neuroinflammation in a mouse model of Parkinson's disease. [Abstract]

2022 Aug 10:12:887407. PMID: 36034698
Microchem J

2026 Mar 18.
Mol Med Rep

Ginsenoside Rg1 alleviates learning and memory impairments and Aβ disposition through inhibiting NLRP1 inflammasome and autophagy dysfunction in APP/PS1 mice. [Abstract]

2023 Jan;27(1):6. PMID: 36367174
Exp Neurol

Trpc6 knockout improves behavioral dysfunction and reduces Aβ production by inhibiting CN-NFAT1 signaling in T2DM mice. [Abstract]

2023 May:363:114350. PMID: 36791875
Exp Neurol

A single closed head injury in male adult mice induces chronic, progressive white matter atrophy and increased phospho-tau expressing oligodendrocytes. [Abstract]

2023 Jan:359:114241. PMID: 36240881
Cell Biol Int

Bone marrow mesenchymal stem cells-derived exosomes reduce Aβ deposition and improve cognitive function recovery in mice with Alzheimer's disease by activating sphingosine kinase/sphingosine-1-phosphate signaling pathway. [Abstract]

2021 Apr;45(4):775-784. PMID: 33300254
Behav Brain Funct

Inhibition of NLRP1 inflammasome improves autophagy dysfunction and Aβ disposition in APP/PS1 mice. [Abstract]

2023 Apr 13;19(1):7. PMID: 37055801
Int J Pept Res Ther

2021 Mar 31.
Folia Neuropathol

Lycopene supplementation protects vascular dementia gerbils against the impairment of learning and memory. [Abstract]

2021;59(2):161-173. PMID: 34284544
bioRxiv

2026 Jul 13.
chemRxiv

2026 Apr 24.
Biomed Pharmacother

Pharmacological interventions on GSK3β phosphorylation-mediated tau aggregation by modulating phase separation of tau proline-rich domain. [Abstract]

2025 Aug:189:118290. PMID: 40580878
Res Sq

2025 May 15.
Research Square Preprint

2023 Jul 13.
Research Square Preprint

2020 Aug.

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : ≥ 50 mg/mL

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. McLellan ME, et al. In vivo imaging of reactive oxygen species specifically associated with thioflavine S-positive amyloid plaques by multiphoton microscopy. J Neurosci. 2003 Mar 15;23(6):2212-7. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

Thioflavine S

31 Cited Publications

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Biologische Aktivität
- Beschreibung
- In Vitro
- Dyeing Example
Chemical Information
Publications (31)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise

1/16
1/16

Thioflavine S purchased from MedChemExpress. Usage Cited in: Nat Aging. 2025 Dec 19. [Abstract]

Customer Validation
Brain sections stained were stained with Thioflavin S (green; 500 μM). DAPI was used for nuclear staining (blue).
1/16

Thioflavine S purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 26;16(1):10514. [Abstract]

Customer Validation
Representative immunofluorescence images showing that mCherry-PCBP2 condensates (red) do not substantially colocalize with the Thioflavin S (ThioS; 0.1% w/v) in SH-SY5Y-mCherry-PCBP2 cells. Arrows in green: amyloid plaques or aggresome, red: PCBP2 condensates.
1/16

Thioflavine S purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2022 Apr;78:103980. [Abstract]

Customer Validation
Thioflavin S (ThS; 500 µM; 8 min). Representative images and quantification analysis of ThS-stained insoluble Aβ plaques in whole brain of 3-5-month-old mice.
1/16

Thioflavine S purchased from MedChemExpress. Usage Cited in: Basic Res Cardiol. 2021 Dec 16;116(1):65. [Abstract]

Customer Validation
Thioflavin S (1 mL/kg; Tail vein injection). Endothelial-specific overexpression of HINT2 protected the cardiac microcirculation against I/R injury by modulating the MCU complex. Mice were transfected with AAV9-NC or AAV9-HINT2 and suffered from 45 min ischemia and 6 h reperfusion injury. Ru360 was injected intraperitoneally into AAV9-NC mice 1 h before I/R injury to inhibit the MCU complex. Spermine was injected intraperitoneally into AAV9-HINT2 mice 1 h before I/R injury to activate the MCU complex. No reflow area after reperfusion was detected by Thioflavin S staining and quantified.
1/16

Thioflavine S purchased from MedChemExpress. Usage Cited in: J Ginseng Res. 2021 Nov;45(6):665-675. [Abstract]

Customer Validation
The Aβ deposition in cortex and hippocamus (1% Thioflavin-S staining, yellow arrows indicate Aβ deposition).

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Thioflavine S

Publications Citing Use of MedChemExpress (MCE) Thioflavine S