Chlorsulfuron
Based on 1 publication(s) in Google Scholar
Chlorsulfuron is an acetolactate synthase inhibitor and selective systemic herbicide. Chlorsulfuron blocks the synthesis of branched-chain amino acids (valine and isoleucine), thereby inhibiting protein synthesis, cell growth, and cell division. Chlorsulfuron blocks G1 and G2 phase cell cycle progression in plant root tip cells without directly interfering with mitosis or DNA synthesis. Chlorsulfuron completely inhibits germination of non-target terrestrial plants and is highly toxic to aquatic macrophytes. Chlorsulfuron has low toxicity to mammals, birds and bees, and moderate toxicity to fish and aquatic invertebrates. Chlorsulfuron can be used in studies related to herbicides, plant cell cycles and ecotoxicology.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 64902-72-3
- Formula: C12H12ClN5O4S
- Molecular Weight:357.77
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Chlorsulfuron
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Biological Activity
Chlorsulfuron (28 nM) prevents the resumption of mitotic progression in excised root tip cells of pea (Pisum sativum L. cv Alaska) after cutting-induced reduction of mitotic activity[1].
Chlorsulfuron (28 nM) reduces the mitotic entry rate to nearly 0% at 8 h post-treatment in pea root tips that are cultured and equilibrated for 24 h after excision, and this inhibitory effect does not recover during the 24 h of continuous observation[1].
Chlorsulfuron (0.047 lb a.i./ac) completely inhibits the germination of Isatis indigotica*[2].
Chlorsulfuron (0.00025 mg/L; 4 weeks) inhibits the growth of Potamogeton pectinatus[2].
Chlorsulfuron (0.0004-0.0007 mg a.i./L; 96 h) shows high toxicity to duckweed, with an EC50 of 0.0007 mg a.i./L and a critical safety threshold of 0.0004 mg a.i./L[2].
Chlorsulfuron (0.001 mg a.i./L; 16 days) reduces the diversity of freshwater algal communities consisting of 63 species[2].
Chlorsulfuron (0.25-2.0 ppb; 4 weeks) exerts adverse effects on the growth and survival of Potamogeton pectinatus plants at all tested concentrations, causing 100% mortality at the concentration of 2.0 ppb[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chlorsulfuron (1363-5545 mg a.i./kg BW; oral gavage; single administration) exhibits low acute oral toxicity in rats and guinea pigs[2].
Chlorsulfuron (75-200 mg a.i./kg BW-day; oral gavage; daily; administered during pregnancy) causes weight loss in pregnant rabbits at high doses, while no effects are observed at medium and low doses[2].
Continuous administration of Chlorsulfuron (5-25 mg/kg body weight/day based on active ingredient; administered via feeding; daily dosing; for 90 days to 2 years) at high doses for 90 days causes changes in hematological parameters in brown rats (Rattus norvegicus), while continuous administration at the same doses for 2 years reduces body weight in male rats; no adverse effects are observed at low doses[2].
Chlorsulfuron (99-5000 mg/kg BW-day; administered via feeding; daily dosing; for 5-14 days, 1 generation; single dosing; for 8 days) shows low acute and chronic toxicity to mallards, with no adverse effects observed[2].
Chlorsulfuron (100-5000 mg/kg BW-day; oral administration via feed; daily; 5-14 days, 27 weeks; single dose; 8 days) shows low acute toxicity to northern bobwhite quails. No adverse reactions are observed at high doses, but reproductive toxicity occurs upon exposure at 560 mg/kg BW-day for 27 weeks, while no toxic effects are detected at low doses[2].
Chlorsulfuron (1.6-25 μg/bee administered via thoracic injection; single dose; 48 h) exhibits mild acute contact toxicity to honeybees[2].
Chlorsulfuron (40 mg/L; aqueous solution exposure; continuous exposure; 96 h) exhibits moderate acute toxicity to brown trout[2].
Chlorsulfuron (31.4-64.8 mg/L; aqueous solution exposure; continuous; 77 days) exerts adverse effects on rainbow trout fry at high concentrations, while no effects are observed at low concentrations[2].
Chlorsulfuron (50-300 mg/L; aqueous solution exposure for 96 h) shows moderate acute toxicity to warm-water fish, with its 96-h LC50 value greater than 50 mg/L[2].
Chlorsulfuron (aqueous solution exposure; continuous exposure; 48 h) exhibits moderate acute toxicity to Daphnia magna, with a 48 h LC50 of 368.9 mg/L[2].
Chlorsulfuron (20-36 mg/L; aqueous solution exposure; continuous treatment; 21 days) exerts chronic adverse effects on Ceriodaphnia dubia at high concentrations, while no effects are observed at low concentrations[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Leporidae sp.[2]
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Dosage:3400 mg a.i./kg BW
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Administration:dermal; single exposure; 24 hours
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Result:Observed no adverse effects from acute dermal exposure.
Determined the acute dermal LD50 is greater than 3400 mg a.i./kg BW.
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Animal Model:Rattus norvegicus[2]
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Dosage:5545 mg a.i./kg BW
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Administration:oral gavage; single dose
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Result:Determined the oral LD50 is 5545 mg a.i./kg BW, meaning this single dose caused 50% mortality in male rats.
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Animal Model:Cavia sp.[2]
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Dosage:1363 mg a.i./kg BW
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Administration:oral gavage; single dose
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Result:Determined the oral LD50 is 1363 mg a.i./kg BW, meaning this single dose caused 50% mortality in guinea pigs.
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Animal Model:Leporidae sp. (pregnant)[2]
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Dosage:200 mg a.i./kg BW-day; 75 mg a.i./kg BW-day
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Administration:oral gavage; daily; during pregnancy
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Result:Caused maternal toxicity (weight loss) at 200 mg a.i./kg BW-day, while observed no adverse effects at 75 mg a.i./kg BW-day.
Noted no treatment-related effects on pregnancy rates or fertility parameters at either dose.
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Animal Model:Rattus norvegicus[2]
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Dosage:25 mg a.i./kg BW-day; 5 mg a.i./kg BW-day
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Administration:dietary; daily; 90 days; 2 years
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Result:Caused adverse effects to blood parameters at 25 mg a.i./kg BW-day in the 90-day study, while observed no effects at 5 mg a.i./kg BW-day.
Observed decreased body weight in male rats at 25 mg a.i./kg BW-day in the 2-year study, with no effects observed at 5 mg a.i./kg BW-day.
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Animal Model:Anas platyrhynchos[2]
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Dosage:500 mg/kg BW-day; >1500 mg/kg BW; 5000 mg/kg BW-day; 99 mg/kg BW-day
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Administration:dietary; daily; 5 days, 1 generation; oral; daily; 14 days; single dose; 8 days
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Result:Observed no toxic effects at 500 mg/kg BW-day in the 5-day dietary study, with an LD50 greater than 1500 mg/kg BW over 8 days.
Observed no adverse effects at 5000 mg/kg BW-day in the 14-day oral study.
Observed no reproductive effects at 99 mg/kg BW-day in the 1-generation dietary study.
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Animal Model:Colinus virginianus[2]
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Dosage:3394 mg/kg BW-day; >16970 mg/kg BW; 5000 mg/kg BW-day; 560 mg/kg BW-day; 100 mg/kg BW-day
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Administration:dietary; daily; 5 days, 27 weeks; oral; daily; 14 days; single dose; 8 days
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Result:Observed no toxic effects at 3394 mg/kg BW-day in the 5-day dietary study, with an LD50 greater than 16970 mg/kg BW over 8 days.
Observed no adverse effects at 5000 mg/kg BW-day in the 14-day oral study.
Caused reproductive toxicity at 560 mg/kg BW-day in the 27-week dietary study, while observed no effects at 100 mg/kg BW-day.
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Animal Model:Apis mellifera[2]
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Dosage:>25 μg/bee; <1.6 μg/bee
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Administration:direct thoracic application; single dose; 48 hours
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Result:Determined the acute contact LD50 is greater than 25 μg/bee, with no observed effects at doses below 1.6 μg/bee.
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Animal Model:Salmo trutta (coldwater fish)[2]
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Dosage:40 mg/L
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Administration:aqueous exposure; continuous; 96 hours
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Result:Determined the acute LC50 is 40 mg/L, meaning this concentration caused 50% mortality over 96 hours.
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Animal Model:Oncorhynchus mykiss (coldwater fish fry, early life stages)[2]
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Dosage:64.8 mg/L; 31.4 mg/L
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Administration:aqueous exposure; continuous; 77 days
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Result:Caused adverse effects to early life stages at 64.8 mg/L, while observed no adverse effects at 31.4 mg/L.
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Animal Model:Pimephales promelas, Lepomis macrochirus, Ictalurus punctatus (warmwater fish)[2]
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Dosage:>50 mg/L to >300 mg/L
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Administration:aqueous exposure; continuous; 96 hours
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Result:Determined acute LC50 values ranged from greater than 50 mg/L to greater than 300 mg/L across species, with no mortality observed at these concentrations.
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Animal Model:Daphnia magna (aquatic invertebrate)[2]
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Dosage:368.9 mg/L
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Administration:aqueous exposure; continuous; 48 hours
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Result:Determined the acute LC50 is 368.9 mg/L, meaning this concentration caused 50% mortality over 48 hours.
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Animal Model:Ceriodaphnia dubia (aquatic invertebrate)[2]
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Dosage:36 mg/L; 20 mg/L
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Administration:aqueous exposure; continuous; 21 days
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Result:Determined the lowest-observed-adverse-effect level (LOAEL) was 36 mg/L, while the no-observed-adverse-effect level (NOAEL) was 20 mg/L over the 21-day life-cycle study.
Chemical Information
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CAS No. 64902-72-3
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Appearance Solid
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Molecular Weight 357.77
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Formula C12H12ClN5O4S
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Color White to off-white
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SMILES
O=S(C1=CC=CC=C1Cl)(NC(NC2=NC(OC)=NC(C)=N2)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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ACS Environ Au
Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]2025 Aug 5;5(6):573-582. PMID: 41277996
Solvent & Solubility
DMSO : 100 mg/mL (279.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.99 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.99 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (289 KB)
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SDS (398 KB)
- English - EN (398 KB)
- Français - FR (398 KB)
- Deutsch - DE (398 KB)
- Norwegian - NO (398 KB)
- Español - ES (398 KB)
- Swedish - SV (398 KB)
- Italian - IT (398 KB)
- Korean - KR (398 KB)
- Portuguese - PT (398 KB)
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Handling Instructions (2659 KB)
References
[1]. Rost TL, et al. Reversal of chlorsulfuron-induced inhibition of mitotic entry by isoleucine and valine. Plant physiology. 1985 Feb;77(2):481-2. [Content Brief]
[3]. Coyner A, et al. Effect of chlorsulfuron on growth of submerged aquatic macrophyte Potamogeton pectinatus (sago pondweed). Environmental pollution (Barking, Essex : 1987). 2001;111(3):453-5. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7951 mL | 13.9755 mL | 27.9509 mL | 69.8773 mL |
| 5 mM | 0.5590 mL | 2.7951 mL | 5.5902 mL | 13.9755 mL | |
| 10 mM | 0.2795 mL | 1.3975 mL | 2.7951 mL | 6.9877 mL | |
| 15 mM | 0.1863 mL | 0.9317 mL | 1.8634 mL | 4.6585 mL | |
| 20 mM | 0.1398 mL | 0.6988 mL | 1.3975 mL | 3.4939 mL | |
| 25 mM | 0.1118 mL | 0.5590 mL | 1.1180 mL | 2.7951 mL | |
| 30 mM | 0.0932 mL | 0.4658 mL | 0.9317 mL | 2.3292 mL | |
| 40 mM | 0.0699 mL | 0.3494 mL | 0.6988 mL | 1.7469 mL | |
| 50 mM | 0.0559 mL | 0.2795 mL | 0.5590 mL | 1.3975 mL | |
| 60 mM | 0.0466 mL | 0.2329 mL | 0.4658 mL | 1.1646 mL | |
| 80 mM | 0.0349 mL | 0.1747 mL | 0.3494 mL | 0.8735 mL | |
| 100 mM | 0.0280 mL | 0.1398 mL | 0.2795 mL | 0.6988 mL |
Chlorsulfuron Related Classifications
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