CN-716 dihydrochloride 

CN-716 dihydrochloride is a reversible covalent flavivirus NS2B·NS3 protease inhibitor with antiviral activity. CN-716 dihydrochloride effectively inhibits the replication of dengue virus (DENV2), West Nile virus (WNV) and Zika virus (ZIKV). The IC₅₀ values of CN-716 dihydrochloride against the proteases of the above three viruses are 0.066 μM, 0.11 μM and 0.25 μM, respectively, while the K_i values against the same proteases are 0.051 μM, 0.082 μM and 0.04 μM, respectively. CN-716 dihydrochloride can be used to study the infection mechanisms of dengue fever, West Nile fever and Zika virus infection^[1][2].

CN-716 dihydrochloride inhibits Dengue virus replication in cell culture with an EC₅₀ of 30 μM^[2].
CN-716 dihydrochloride (up to 100 μM) shows no cytotoxicity in cell culture at concentrations up to 100 μM^[2].
CN-716 dihydrochloride binds to West Nile virus NS2B·NS3 protease in the closed active conformation, forming a covalent bond with Ser135 and multiple specific non-covalent interactions with the protease subsites^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

527.25

C₂₂H₃₃BCl₂N₆O₄

2077164-48-6

NCC(C=C1)=CC=C1C[C@H](NC(C2=CC=CC=C2)=O)C(N[C@H](B(O)O)CCCNC(N)=N)=O.Cl.Cl

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Mahawaththa M C, et al. Solution conformations of a linked construct of the Zika virus NS2B-NS3 protease[J]. Antiviral research, 2017, 142: 141-147.

  [2]. Nitsche C, et al. Peptide-Boronic Acid Inhibitors of Flaviviral Proteases: Medicinal Chemistry and Structural Biology. J Med Chem. 2017;60(1):511-516.  [Content Brief]