CEBP beta Antibody (YA576)
(Synonyms: TCF5, PP9092, CEBPB, CCAAT/enhancer-binding protein beta, C/EBP beta, Liver activator protein, Liver-enriched inhibitory protein, Nuclear factor NF-IL6, Transcription factor 5, LAP, LIP, TCF-5)
Based on 1 Customer Validation
CEBP beta Antibody (YA576) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CEBP beta.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, FC, IP
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Reactivity :
Human, Rat
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Formulation:
Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:50-1:100 | Use at an assay dependent concentration. |
Product Details
CEBP beta Antibody (YA576) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CEBP beta.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 36 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 36 kDa
Entrez Gene: 1051 Human ; 12608 Mouse ; 24253 Rat
SwissProt: P17676 Human ; P28033 Mouse ; P21272 Rat
OMIM: 189965 Human
Synthetic peptide corresponding to Rat C/EBP Beta.AA range:248-297.
Endogenous
Protein A affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CEBP beta is important transcription factor regulating the expression of genes involved in immune and inflammatory responses. Also plays a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant roles with CEBPA. Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Also plays a role in intracellular bacteria killing. During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation. Essential for female reproduction because of a critical role in ovarian follicle development. Restricts osteoclastogenesis: together with NFE2L1; represses expression of DSPP during odontoblast differentiation; Essential for gene expression induction in activated macrophages. Plays a major role in immune responses such as CD4(+) T-cell response, granuloma formation and endotoxin shock. Not essential for intracellular bacteria killing; Acts as a dominant negative through heterodimerization with isoform 2. Promotes osteoblast differentiation and osteoclastogenesis[1][2][3][4][5][6].
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Subcellular Localization
Nucleus; Cytoplasm
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Expression
Tissue_specificity:Expressed at low levels in the lung, kidney and spleen -
Subunit
By ER stress
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SwissProt ID
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Synonyms
TCF5, PP9092, CEBPB, CCAAT/enhancer-binding protein beta, C/EBP beta, Liver activator protein, Liver-enriched inhibitory protein, Nuclear factor NF-IL6, Transcription factor 5, LAP, LIP, TCF-5
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
References
[1]. Roy SK, et al. MEKK1 plays a critical role in activating the transcription factor C/EBP-beta-dependent gene expression in response to IFN-gamma. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7945-50. [Content Brief]
[2]. Kinoshita S, et al. A member of the C/EBP family, NF-IL6 beta, forms a heterodimer and transcriptionally synergizes with NF-IL6. Proc Natl Acad Sci U S A. 1992 Feb 15;89(4):1473-6. [Content Brief]
[3]. Pless O, et al. G9a-mediated lysine methylation alters the function of CCAAT/enhancer-binding protein-beta. J Biol Chem. 2008 Sep 26;283(39):26357-63. [Content Brief]
[4]. Chinery R, et al. Antioxidant-induced nuclear translocation of CCAAT/enhancer-binding protein beta. A critical role for protein kinase A-mediated phosphorylation of Ser299. J Biol Chem. 1997 Nov 28;272(48):30356-61. [Content Brief]
[5]. Park SH, et al. Endoplasmic reticulum stress-activated C/EBP homologous protein enhances nuclear factor-kappaB signals via repression of peroxisome proliferator-activated receptor gamma. J Biol Chem. 2010 Nov 12;285(46):35330-9. [Content Brief]
[6]. Zhu Y, et al. Dynamic regulation of cyclooxygenase-2 promoter activity by isoforms of CCAAT/enhancer-binding proteins. J Biol Chem. 2002 Mar 1;277(9):6923-8. [Content Brief]
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