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Glucose 6 Phosphate Dehydrogenase Antibody (YA411)

(Synonyms: G6PD; Glucose-6-phosphate 1-dehydrogenase; G6PD)
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Based on 1 Customer Validation

Glucose 6 Phosphate Dehydrogenase Antibody (YA411) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucose 6 Phosphate Dehydrogenase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Glucose 6 Phosphate Dehydrogenase Antibody (YA411)
  • Glucose 6 Phosphate Dehydrogenase Antibody (YA411) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:200-1:2000 1:100 1:200-1:500 1:20

Product Details

Description

Glucose 6 Phosphate Dehydrogenase Antibody (YA411) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucose 6 Phosphate Dehydrogenase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 59 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 59 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Glucose 6 Phosphate Dehydrogenase.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102667

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glucose 6 Phosphate Dehydrogenase Antibody (YA411)
    Western blot analysis of extracts from K562 (lane 1) and Hela (lane 2) and C212 (lane 3) and C6 (lane 4) using Glucose 6 Phosphate Dehydrogenase antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    Glucose 6 Phosphate Dehydrogenase catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis

  • Subcellular Localization

    Cytoplasm, cytosol; Membrane; Peripheral membrane protein

  • Expression


    Tissue_specificity:The long subtype is found in lymphoblasts, granulocytes, and sperm.

  • Isoforms & Post-Translational Modification

    P11413 has 3 isomers: P11413-1: 59257 Da (predicted); P11413-2: 63827 Da (predicted); P11413-3: 62468 Da (predicted).
    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity

  • Subunit

    Homotetramer; dimer of dimers (PubMed:10745013, PubMed:15858258, PubMed:24769394, PubMed:38066190). Interacts with SIRT2; the interaction is enhanced by H(2)O(2) treatment (PubMed:24769394). Forms a ternary complex with ALDOB and TP53; this interaction is direct. ALDOB stabilizes the complex inhibiting G6PD activity and keeping oxidative pentose phosphate metabolism in check

  • SwissProt ID

    P11413

  • Gene ID
    2539 [NCBI]
  • Synonyms

    G6PD; Glucose-6-phosphate 1-dehydrogenase; G6PD

  • Research Field

    Signal Transduction

Glucose 6 Phosphate Dehydrogenase Antibody (YA411) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 1) and Hela (lane 2) and C212 (lane 3) and C6 (lane 4) using Glucose 6 Phosphate Dehydrogenase antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Glucose 6 Phosphate Dehydrogenase Antibody (YA411) Product Image
    1/16
  • Experimental Validation Results for Glucose 6 Phosphate Dehydrogenase Antibody (YA411)
  • Glucose 6 Phosphate Dehydrogenase Antibody (YA411) Product Image
  • Experimental Validation Results for Glucose 6 Phosphate Dehydrogenase Antibody (YA411)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 1) and Hela (lane 2) and C212 (lane 3) and C6 (lane 4) using Glucose 6 Phosphate Dehydrogenase antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Glucose 6 Phosphate Dehydrogenase Antibody (YA411)
  • Glucose 6 Phosphate Dehydrogenase Antibody (YA411) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562 (lane 1) and Hela (lane 2) and C212 (lane 3) and C6 (lane 4) using Glucose 6 Phosphate Dehydrogenase antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Glucose 6 Phosphate Dehydrogenase Antibody (YA411) Product Image WB