D-Mannoheptulose
Based on 1 Customer Validation
D-Mannoheptulose is a 7-carbon keto sugar found in avocado. D-mannoheptulose is a specific inhibitor of D-glucose phosphorylation by hexokinase isoenzymes. D-Mannoheptulose can block insulin release and utilization of carbohydrate in rat. D-Mannoheptulose has various functions as anti-oxidants, anticancer effects and energy sources.
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- Pureté : 99.9%
- CAS No.: 3615-44-9
- Formule: C7H14O7
- Masse moléculaire:210.18
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Stockage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
D-Mannoheptulose (1250-39 µg/mL; 72 h) has IC50 values of 975.1 µg/mL and 263.3 µg/mL for normal HMECs cells and breast cancer cells (MCF-7), respectively[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7 cells and normal human mammary epithelial cells (HMECs)
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Concentration:(1250, 625, 312.5, 156.3, 78, and 39 µg/mL) (6 µM, 3 µM, 1.5 µM, 0.75 µM, 0.375 µM, and 0.1875 µM)
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Incubation Time:72 h
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Result:Can result in inhibiting the growth of breast cancer cell lines.
There was no significant cytotoxic effect on normal cells.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:The rats and sand rats[3]
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Dosage:20% w/v
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Administration:SC injection; once
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Result:Blocked utilization of carbohydrate.
The fat catabolism in the fasted sand rat reaches the same intensity as in the rat.
Chemical Information
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CAS No. 3615-44-9
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Appearance Solid
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Masse moléculaire 210.18
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Formule C7H14O7
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Color White to off-white
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SMILES
OCC([C@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O)=O
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvant et solubilité
In Vitro:
DMSO : 125 mg/mL (594.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.89 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.89 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (273 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
[2]. Courtois P, et al. D-mannoheptulose phosphorylation by hexokinase isoenzymes. Int J Mol Med. 2001 Apr;7(4):359-63. [Content Brief]
[3]. Frenkel G, et al. Metabolic effects of insulin blockade by D-mannoheptulose in the sand rat (Psammomys obesus) and the rat. Gen Comp Endocrinol. 1972 Oct;19(2):341-4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.7578 mL | 23.7891 mL | 47.5783 mL | 118.9457 mL |
| 5 mM | 0.9516 mL | 4.7578 mL | 9.5157 mL | 23.7891 mL | |
| 10 mM | 0.4758 mL | 2.3789 mL | 4.7578 mL | 11.8946 mL | |
| 15 mM | 0.3172 mL | 1.5859 mL | 3.1719 mL | 7.9297 mL | |
| 20 mM | 0.2379 mL | 1.1895 mL | 2.3789 mL | 5.9473 mL | |
| 25 mM | 0.1903 mL | 0.9516 mL | 1.9031 mL | 4.7578 mL | |
| 30 mM | 0.1586 mL | 0.7930 mL | 1.5859 mL | 3.9649 mL | |
| 40 mM | 0.1189 mL | 0.5947 mL | 1.1895 mL | 2.9736 mL | |
| 50 mM | 0.0952 mL | 0.4758 mL | 0.9516 mL | 2.3789 mL | |
| 60 mM | 0.0793 mL | 0.3965 mL | 0.7930 mL | 1.9824 mL | |
| 80 mM | 0.0595 mL | 0.2974 mL | 0.5947 mL | 1.4868 mL | |
| 100 mM | 0.0476 mL | 0.2379 mL | 0.4758 mL | 1.1895 mL |
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