Dichloro-all-trans-retinone
Dichloro-all-trans-retinone is a selective retinaldehyde dehydrogenase (RALDH) inhibitor, with an IC50 of 434.7 nM and a Ki of 194.4 nM against chicken RALDH1; an IC50 of 55 nM and a Ki of 5.1 nM against chicken RALDH2; an IC50 of 191.32 nM and a Ki of 64.35 nM against human RALDH2; and an IC50 of 161.27 nM and a Ki of 6.74 nM against chicken RALDH3. Dichloro-all-trans-retinone inhibits the synthesis of all-trans retinoic acid (ATRA). Dichloro-all-trans-retinone is applicable to research related to form-deprivation-induced myopia.
For research use only. We do not sell to patients.
- CAS No.: 2089135-65-7
- Formula: C21H28Cl2O
- Molecular Weight:367.35
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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hRALDH2 191.32 nM (IC50) |
hRALDH2 64.35 nM (Ki) |
cRALDH1 434.7 nM (IC50) |
cRALDH1 194.4 nM (Ki) |
cRALDH2 55 nM (IC50) |
cRALDH2 5.1 nM (Ki) |
cRALDH3 161.27 nM (IC50) |
cRALDH3 6.74 nM (Ki) |
Dichloro-all-trans-retinone (0-10 μM; 20 min pre-incubation) potently inhibits recombinant chicken RALDH1, RALDH2, RALDH3 and human RALDH2 in vitro, with the lowest IC50 value of 55 nM observed for chicken RALDH2[1].
Dichloro-all-trans-retinone (0.10-6 μM; 20 min pre-incubation) potently inhibits RALDH2 activity in the choroidal cytosolic fractions of control chicks and chicks during myopia recovery, with an IC50 of 53.60 nM in choroidal lysates from the recovery period[1].
Dichloro-all-trans-retinone (0-5 μM; 24 hrs organ culture) inhibits ATRA synthesis by 42% in intact, living choroidal tissues from chick eyes during myopia recovery, with an EC50 of 119.70 nM, but exerts no effect on choroidal tissues from control eyes[1].
Dichloro-all-trans-retinone (0-50 μM; 24 hrs) inhibits doxycycline (HY-N0565)-induced ATRA synthesis in RALDH2-eGFP HEK-293 cells, with an IC50 of 187.20 nM normalized to RALDH2 expression levels, and exhibits cytotoxicity at concentrations above approximately 3 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:doxycycline-inducible (Dox)RALDH2-eGFP HEK-293 cells expressing chicken RALDH2
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Concentration:0-50 μM
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Incubation Time:24 h
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Result:Reduced cell viability with IC50 values of 2.94 μM (DNA content) and 3.09 μM (ATP production).
Chemical Information
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CAS No. 2089135-65-7
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Molecular Weight 367.35
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Formula C21H28Cl2O
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SMILES
C(=C/C(=C/C=C/C(=C/C(C(Cl)Cl)=O)/C)/C)\C=1C(C)(C)CCCC1C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Dichloro-all-trans-retinone Related Classifications
HY-182948 Related Classifications
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