DiOC7(3)  (Synonyms: 3,3'-Diheptyloxacarbocyanine iodide)

DiOC7(3) (3,3'-Diheptyloxacarbocyanine iodide) is a green membrane potential probe (Ex=450-490 nm, Em=510-520 nm). DiOC7(3) can be used to quantify the vascular densities^[1].

Identification for tumour vasculature (frozen section):
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[1].
1. The dye is administered to murine via lateral tail vein injection at a dose of 1 mg/kg/ in 50 μL volume.
2. Animals are killed 5 min after dye administration, and tumors are immediately excised and frozen at - 20°C.
3. Frozen sections 10 μm in thickness are rewarmed to room temperature, allowed to air dry.
4. Image using a microscope with 100W mercury lamp illumination and an epifluorescence condenser (excited at 430-490 nm, emitted at 510-520 nm.

600.57

C₃₁H₄₁IN₂O₂

53213-83-5

510

475

CCCCCCCN1/C(OC2=CC=CC=C12)=C/C=C/C3=[N+](CCCCCCC)C4=CC=CC=C4O3.[I-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. M J Trotter, et al. Use of a carbocyanine dye as a marker of functional vasculature in murine tumours. Br J Cancer. 1989 May;59(5):706-9.  [Content Brief]