Cat. No.: HY-121035

7BIO

(Synonyms: 7-Bromoindirubin-3-Oxime)

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Based on 1 Customer Validation

7BIO (7-Bromoindirubin-3-Oxime) is the derivate of indirubin. 7BIO (7-Bromoindirubin-3-Oxime) has inhibitory effects against cyclin-dependent kinase-5 (CDK5) and glycogen synthase kinase-3β (GSK3β). 7BIO (7-Bromoindirubin-3-Oxime) inhibits Aβ oligomer-induced neuroinflammation, synaptic impairments, tau hyper-phosphorylation, activation of astrocytes and microglia, and attenuates Aβ oligomer-induced cognitive impairments in mice^[1].

For research use only. We do not sell to patients.

Purity: 99.20%
CAS No.: 916440-85-2
Formula: C₁₆H₁₀BrN₃O₂
Molecular Weight:356.17

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

7BIO Chemical Structure

CAS No.: 916440-85-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

CDK5

GSK3β

All CDK Isoforms :

All GSK-3 Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	13.9 μM Compound: 5e; 7-BIO	Antiproliferative activity against apoptosis resistant human A549 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis resistant human A549 cells after 72 hrs by MTT assay	[PMID: 26883150]
A549	IC₅₀	21.8 μM Compound: 7d	Survival of human A549 cells after 48 hrs by MTS reduction assay Survival of human A549 cells after 48 hrs by MTS reduction assay	[PMID: 16854069]
B16	IC₅₀	8 μM Compound: 7d	Survival of mouse F1 cells after 48 hrs by MTS reduction assay Survival of mouse F1 cells after 48 hrs by MTS reduction assay	[PMID: 16854069]
B16-F10	IC₅₀	5.3 μM Compound: 5e; 7-BIO	Antiproliferative activity against apoptosis sensitive mouse B16F10 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis sensitive mouse B16F10 cells after 72 hrs by MTT assay	[PMID: 26883150]
BP8	IC₅₀	19.4 μM Compound: 7d	Survival of mouse AhR -/- BP8 cells after 48 hrs by MTS reduction assay Survival of mouse AhR -/- BP8 cells after 48 hrs by MTS reduction assay	[PMID: 16854069]
HCT-116	IC₅₀	6.2 μM Compound: 7d	Survival of human HCT116 cells after 48 hrs by MTS reduction assay Survival of human HCT116 cells after 48 hrs by MTS reduction assay	[PMID: 16854069]
HEK293	IC₅₀	12.3 μM Compound: 7d	Survival of human HEK293 cells after 48 hrs by MTS reduction assay Survival of human HEK293 cells after 48 hrs by MTS reduction assay	[PMID: 16854069]
HepG2	IC₅₀	4.8 μM Compound: 4b	Cytotoxicity against human HepG2 cells assessed as cell growth inhibition after 24 hrs by alamar blue assay Cytotoxicity against human HepG2 cells assessed as cell growth inhibition after 24 hrs by alamar blue assay	[PMID: 28743492]
Hs 683	IC₅₀	8.1 μM Compound: 5e; 7-BIO	Antiproliferative activity against apoptosis sensitive human Hs683 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis sensitive human Hs683 cells after 72 hrs by MTT assay	[PMID: 26883150]
HT-29	IC₅₀	21.2 μM Compound: 7d	Survival of human HT29 cells after 48 hrs by MTS reduction assay Survival of human HT29 cells after 48 hrs by MTS reduction assay	[PMID: 16854069]
Huh-7	IC₅₀	9 μM Compound: 7d	Survival of human Huh7 cells after 48 hrs by MTS reduction assay Survival of human Huh7 cells after 48 hrs by MTS reduction assay	[PMID: 16854069]
LoVo	IC₅₀	5.6 μM Compound: 5e; 7-BIO	Antiproliferative activity against human LoVo cells after 72 hrs by MTT assay Antiproliferative activity against human LoVo cells after 72 hrs by MTT assay	[PMID: 26883150]
MCF7	IC₅₀	4.9 μM Compound: 5e; 7-BIO	Antiproliferative activity against apoptosis sensitive human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis sensitive human MCF7 cells after 72 hrs by MTT assay	[PMID: 26883150]
MDA-MB-231	IC₅₀	10.5 μM Compound: 7d	Survival of human MDA-MB-231 cells after 48 hrs by MTS reduction assay Survival of human MDA-MB-231 cells after 48 hrs by MTS reduction assay	[PMID: 16854069]
Oocyte	IC₅₀	22 μM Compound: 54; 55	Inhibition of starfish oocyte CDK1/Cyclin B using histone H1 as substrate incubated for 10 mins in presence of [gamma-32P]ATP by scintillation counter analysis Inhibition of starfish oocyte CDK1/Cyclin B using histone H1 as substrate incubated for 10 mins in presence of [gamma-32P]ATP by scintillation counter analysis	[PMID: 34161865]
PC-3	IC₅₀	19.8 μM Compound: 7d	Survival of human PC3 cells after 48 hrs by MTS reduction assay Survival of human PC3 cells after 48 hrs by MTS reduction assay	[PMID: 16854069]
PC-3	IC₅₀	7.8 μM Compound: 5e; 7-BIO	Antiproliferative activity against apoptosis sensitive human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis sensitive human PC3 cells after 72 hrs by MTT assay	[PMID: 26883150]
SH-SY5Y	EC₅₀	8 μM Compound: 2, 7BIO	Anticancer activity against human SH-SY5Y cells assessed as reduction in cell viability Anticancer activity against human SH-SY5Y cells assessed as reduction in cell viability	[PMID: 24697244]
SH-SY5Y	IC₅₀	7 μM Compound: 7d	Death of human SH-SY5Y cells in absence of 20 uM Q-VD-OPh by MTS reduction assay Death of human SH-SY5Y cells in absence of 20 uM Q-VD-OPh by MTS reduction assay	[PMID: 16854069]
SH-SY5Y	IC₅₀	7 μM Compound: 7d	Death of human SH-SY5Y cells in presence of 20 uM Q-VD-OPh by MTS reduction assay Death of human SH-SY5Y cells in presence of 20 uM Q-VD-OPh by MTS reduction assay	[PMID: 16854069]
SH-SY5Y	IC₅₀	7.1 μM Compound: 7d	Survival of human SH-SY5Y cells after 48 hrs by MTS reduction assay Survival of human SH-SY5Y cells after 48 hrs by MTS reduction assay	[PMID: 16854069]
SH-SY5Y	IC₅₀	8 μM Compound: 7d	Survival of human SH-SY5Y cells after 24 hrs by MTS reduction assay Survival of human SH-SY5Y cells after 24 hrs by MTS reduction assay	[PMID: 16854069]
SK-MEL-28	IC₅₀	9.8 μM Compound: 5e; 7-BIO	Antiproliferative activity against apoptosis resistant human SK-MEL-28 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis resistant human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 26883150]
U-373MG ATCC	IC₅₀	6.6 μM Compound: 5e; 7-BIO	Antiproliferative activity against apoptosis resistant human U373 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis resistant human U373 cells after 72 hrs by MTT assay	[PMID: 26883150]

In Vitro

7BIO (1 and 10 μM; 24 hours) has neuroprotective effects and can prevent, but cannot rescue Aβ1 42 oligomer-induced cell death in SH-SY5Y cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

SH-SY5Y cells
Concentration:

1 and 10 μM
Incubation Time:

24 hours
Result:

Inhibit Aβ oligomer-induced neuronal death. 7BIO prevented, but did not rescue Aβ1−42 oligomer-induced cell death in SH-SY5Y cells.

In Vivo

7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection) significantly attenuates Aβ oligomer-induced impairment of recognition, spatial learning and memory in mice^[1].
7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection) decreases Aβ oligomer-induced increase of TNF-α and IL-6 production in the brain and the expression of synapsin-1 and PSD-95 in the hippocampal region of mice^[1].
7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection) attenuates Aβ oligomer-induced increase expression of pTau, activation of microglia and astrogliosis in the brain of mice^[1].
7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection) prevents decreased expression of pSer9-GSK3β and has no significant effects on the Tau protein level^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

8 weeks mice (30 g)^[1]
Dosage:

2.3, 7.0, and 23.3 μg/kg
Administration:

bilateral ventricle injection
Result:

Attenuates Aβ oligomer-induced impairment of recognition, spatial learning and memory in mice.

Chemical Information

CAS No. 916440-85-2
Appearance Solid
Molecular Weight 356.17
Formula C₁₆H₁₀BrN₃O₂
Color Brown to reddish brown
SMILES

O/N=C1C2=CC=CC=C2NC\1=C3C(NC4=C(C=CC=C4/3)Br)=O
Synonyms

7-Bromoindirubin-3-Oxime
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (701.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 50 mg/mL (140.38 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8076 mL

14.0382 mL

28.0765 mL

5 mM

0.5615 mL

2.8076 mL

5.6153 mL

10 mM

0.2808 mL

1.4038 mL

2.8076 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (5.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (544 KB)
- English - EN (544 KB)
- Français - FR (544 KB)
- Deutsch - DE (544 KB)
- Norwegian - NO (544 KB)
- Español - ES (544 KB)
- Swedish - SV (544 KB)
- Italian - IT (544 KB)
- Korean - KR (544 KB)
- Portuguese - PT (544 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chen L, et al. Indirubin Derivative 7-Bromoindirubin-3-Oxime (7Bio) Attenuates Aβ Oligomer-Induced Cognitive Impairments in Mice. Front Mol Neurosci. 2017;10:393. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO	1 mM	2.8076 mL	14.0382 mL	28.0765 mL	70.1912 mL
	5 mM	0.5615 mL	2.8076 mL	5.6153 mL	14.0382 mL
	10 mM	0.2808 mL	1.4038 mL	2.8076 mL	7.0191 mL
	15 mM	0.1872 mL	0.9359 mL	1.8718 mL	4.6794 mL
	20 mM	0.1404 mL	0.7019 mL	1.4038 mL	3.5096 mL
	25 mM	0.1123 mL	0.5615 mL	1.1231 mL	2.8076 mL
	30 mM	0.0936 mL	0.4679 mL	0.9359 mL	2.3397 mL
	40 mM	0.0702 mL	0.3510 mL	0.7019 mL	1.7548 mL
	50 mM	0.0562 mL	0.2808 mL	0.5615 mL	1.4038 mL
	60 mM	0.0468 mL	0.2340 mL	0.4679 mL	1.1699 mL
	80 mM	0.0351 mL	0.1755 mL	0.3510 mL	0.8774 mL
	100 mM	0.0281 mL	0.1404 mL	0.2808 mL	0.7019 mL

Keywords

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7BIO

Cell Cycle/DNA Damage Compound Library

Kinase Inhibitor Library

PI3K/Akt/mTOR Compound Library

Protein Tyrosine Kinase Compound Library

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

Anti-Aging Compound Library

Reprogramming Compound Library

Oxygen Sensing Compound Library

Glycolysis Compound Library

Cytoskeleton Compound Library

Anti-Alzheimer's Disease Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

Glucose Metabolism Compound Library

Antidepressant Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Metabolic Enzyme Compound Library

Highly Selective Inhibitors Library

Serine/Threonine Kinase Inhibitor Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

High-Efficiency Gene Editing Compound Library

All CDK Isoforms

All GSK-3 Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

7BIO Related Classifications

Cat. No.: HY-121035