GCLE
Based on 1 Customer Validation
GCLE (Cephalosporin G 3-chloromethyl p-methoxybenzyl ester) is a SARS-CoV-2 main protease (Mpro) inhibitor with an IC50 of 0.03 μM. GCLE selectively acylates the Cys145 residue of Mpro. GCLE is applicable to research related to COVID-19.
For research use only. We do not sell to patients.
- Purity: ≥97.0%
- CAS No.: 104146-10-3
- Formula: C24H23ClN2O5S
- Molecular Weight:486.97
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
GCLE (5-20 μM) modestly reduces SARS-CoV-2 replication in infected VeroE6 cells at 20 μM, though it shows no activity at 5 μM and exhibits cytotoxicity at active concentrations[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 104146-10-3
-
Appearance Solid
-
Molecular Weight 486.97
-
Formula C24H23ClN2O5S
-
SMILES
O=C(N[C@@H]1C(N2C(C(OCC3=CC=C(OC)C=C3)=O)=C(CS[C@H]12)CCl)=O)CC4=CC=CC=C4
-
Synonyms
Cephalosporin G 3-chloromethyl p-methoxybenzyl ester
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (205.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (271 KB)
-
SDS (477 KB)
- English - EN (477 KB)
- Français - FR (477 KB)
- Deutsch - DE (477 KB)
- Norwegian - NO (477 KB)
- Español - ES (477 KB)
- Swedish - SV (477 KB)
- Italian - IT (477 KB)
- Korean - KR (477 KB)
- Portuguese - PT (477 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0535 mL | 10.2676 mL | 20.5351 mL | 51.3379 mL |
| 5 mM | 0.4107 mL | 2.0535 mL | 4.1070 mL | 10.2676 mL | |
| 10 mM | 0.2054 mL | 1.0268 mL | 2.0535 mL | 5.1338 mL | |
| 15 mM | 0.1369 mL | 0.6845 mL | 1.3690 mL | 3.4225 mL | |
| 20 mM | 0.1027 mL | 0.5134 mL | 1.0268 mL | 2.5669 mL | |
| 25 mM | 0.0821 mL | 0.4107 mL | 0.8214 mL | 2.0535 mL | |
| 30 mM | 0.0685 mL | 0.3423 mL | 0.6845 mL | 1.7113 mL | |
| 40 mM | 0.0513 mL | 0.2567 mL | 0.5134 mL | 1.2834 mL | |
| 50 mM | 0.0411 mL | 0.2054 mL | 0.4107 mL | 1.0268 mL | |
| 60 mM | 0.0342 mL | 0.1711 mL | 0.3423 mL | 0.8556 mL | |
| 80 mM | 0.0257 mL | 0.1283 mL | 0.2567 mL | 0.6417 mL | |
| 100 mM | 0.0205 mL | 0.1027 mL | 0.2054 mL | 0.5134 mL |
GCLE Related Classifications
HY-W018563 Related Classifications
Powered by Bioz