2. Cell Cycle/DNA Damage Cytoskeleton Apoptosis
  3. Microtubule/Tubulin Apoptosis
  4. IDO/Tubulin-IN-2

IDO/Tubulin-IN-2 (HT2) is a potent TDO and tubulin inhibitor. IDO/Tubulin-IN-2 also shows potent activity against U87, HepG2, A549, HCT-116, and LO2 cancer cell lines, with IC50 values of 0.43, 0.036, 0.041, 0.095 and 1.04 μM, respectively. IDO/Tubulin-IN-2 remarkably promotes the antitumor activity.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

IDO/Tubulin-IN-2

IDO/Tubulin-IN-2 構造式

CAS 番号 : 2409479-24-7

容量 在庫状況
50 mg   お問い合わせ  
100 mg   お問い合わせ  
250 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

IDO/Tubulin-IN-2 関連抗体

Top Publications Citing Use of Products

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

  • カスタマーレビュー

製品説明

IDO/Tubulin-IN-2 (HT2) is a potent TDO and tubulin inhibitor. IDO/Tubulin-IN-2 also shows potent activity against U87, HepG2, A549, HCT-116, and LO2 cancer cell lines, with IC50 values of 0.43, 0.036, 0.041, 0.095 and 1.04 μM, respectively. IDO/Tubulin-IN-2 remarkably promotes the antitumor activity[1].

IC50 & Target

TDO, Tubulin[1]
Cellular Effect
Cell Line Type Value Description References
A549 IC50
0.041 μM
Compound: HT2
Antiproliferative activity against human A549 cells assessed as reduction in cell growth measured after 72 hrs by MTT assay
Antiproliferative activity against human A549 cells assessed as reduction in cell growth measured after 72 hrs by MTT assay
[PMID: 31830637]
HCT-116 IC50
0.095 μM
Compound: HT2
Antiproliferative activity against human HCT116 cells assessed as reduction in cell growth measured after 72 hrs by MTT assay
Antiproliferative activity against human HCT116 cells assessed as reduction in cell growth measured after 72 hrs by MTT assay
[PMID: 31830637]
HepG2 IC50
0.036 μM
Compound: HT2
Antiproliferative activity against human HepG2 cells assessed as reduction in cell growth measured after 72 hrs by MTT assay
Antiproliferative activity against human HepG2 cells assessed as reduction in cell growth measured after 72 hrs by MTT assay
[PMID: 31830637]
L02 IC50
1.04 μM
Compound: HT2
Cytotoxicity against human LO2 cells assessed as reduction in cell growth measured after 72 hrs by MTT assay
Cytotoxicity against human LO2 cells assessed as reduction in cell growth measured after 72 hrs by MTT assay
[PMID: 31830637]
U-87MG ATCC IC50
0.43 μM
Compound: HT2
Antiproliferative activity against human U87 cells assessed as reduction in cell growth measured after 72 hrs by MTT assay
Antiproliferative activity against human U87 cells assessed as reduction in cell growth measured after 72 hrs by MTT assay
[PMID: 31830637]
体外実験

IDO/Tubulin-IN-2 (HT2) (0-50 μM, 4 h) shows potent cytotoxicity with IC50 values between 0.036 and 0.43 μM against cancer cell lines[1].
IDO/Tubulin-IN-2 (0.1 μM, 24 h) arrests the HepG2 cells cycle mainly at the G2 phase[1].
IDO/Tubulin-IN-2 (0.1 μM, 24 h) can effectively cause cell apoptosis[1].
IDO/Tubulin-IN-2 (0.1 μM, 24 h) has strongly effects on inducing the proteolytic cleavage of PARP and up-regulating the expression level of caspase-3[1].
IDO/Tubulin-IN-2 (0.05 μM, 24, 48 and 72 h) markedly decreases mRNA expression level of TDO at a time-dependent manner[1].
IDO/Tubulin-IN-2 (2 days) can improve T-cell activation and proliferation and enhance immune response[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

IDO/Tubulin-IN-2 関連抗体

Cell Proliferation Assay

Cell Line: Human cancer cell lines and non-tumoral cell line[1]
Concentration: 0-50 μM
Incubation Time: 4 h
Result: Displayed potent cytotoxicity with IC50 values of 0.43 μM (U87), 0.036 μM (HepG2), 0.041 μM (A549), 0.095 μM (HCT-116), and 1.04 μM (LO2).

Cell Cycle Analysis

Cell Line: HepG2 cells[1]
Concentration: 0.1 μM
Incubation Time: 24 h
Result: Arrested the HepG2 cells cycle mainly at the G2 phase.

Apoptosis Analysis

Cell Line: HepG2 cells[1]
Concentration: 0.1 μM
Incubation Time: 24 h
Result: Effectively caused cell apoptosis, the percentage of apoptosis cells increased to 54%

Western Blot Analysis

Cell Line: HepG2 cells[1]
Concentration: 0.1 μM
Incubation Time: 24 h
Result: Showed strongly effects on inducing the proteolytic cleavage of PARP and up-regulating the expression level of caspase-3, which could lead to cell death at last.

RT-PCR

Cell Line: HepG2 cells[1]
Concentration: 0.05 μM
Incubation Time: 24, 48 and 72 h
Result: Markedly decreased mRNA expression level of TDO at a time-dependent manner.
体内実験

IDO/Tubulin-IN-2 (HT2) (30 mg/kg; IV; daily, for 21 days) significantly inhibits tumor growth[1].
IDO/Tubulin-IN-2 (30 mg/kg; IV; 29 days) has effective antitumor immunity ability to promote the tumor therapeutic efficacy[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ICR mice (mouse liver cancer xenograft models, established by subcutaneous inoculation of H22 cells)[1]
Dosage: 30 mg/kg
Administration: Intravenously injected via a tail vein; daily, for 21 days
Result: Significantly inhibited tumor growth.
Animal Model: Male A549 tumor xenograft BALB/c nude mice (5 weeks, 18-22 g)[1]
Dosage: 30 mg/kg
Administration: IV, daily, for 29 days
Result: Had effective antitumor immunity ability to promote the tumor therapeutic efficacy.
分子量

860.87

分子式

C48H40N6O10
CAS 番号
SMILES

COC1=CC=C(C=C1OC(C2=CC=CC(CN3C=C(N=N3)CCOC(C4=CC=CC(CN5N=NC6=C5C(C7=CC=CC=C7C6=O)=O)=C4)=O)=C2)=O)/C=C\C8=CC(OC)=C(C(OC)=C8)OC
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

IDO/Tubulin-IN-2 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IDO/Tubulin-IN-22409479-24-7Microtubule/TubulinApoptosisAntitumorHepG2A549 cellsLow toxicityICR miceBALB/c nude miceInhibitorinhibitorinhibit

最近チェックした製品:

オンラインお問い合わせ

Your information is safe with us. * Required Fields.

製品名

  

カスタマ需要量 *

お名前 *

 

タイトル

メールアドレス *

 

電話番号 *

デパートメント

 

組纖名 *

市区町村

都道府県

国或いは地域 *

      

必ず会社名を記載ください。個人への返信は行いません。

備考

バルクお問い合わせ

Inquiry Information

製品名:
IDO/Tubulin-IN-2
製品番号:
HY-146715
数量:
MCE 日本正規代理店:

カスタマーレビュー

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

製品名

  

Lot #

お名前 *

 

メールアドレス *

電話番号 *

 

部門 *

組纖名 *

 

国或いは地域 *

備考

Request for HNMR Report

We have received your request and will respond to you as soon as possible.