製品番号: HY-N1433

Phytolaccagenin

(別名: フィトラッカゲニン)

カスタマーレビュー

Based on 1 Customer Validation

Phytolaccagenin is a triterpenoid saponin aglycone, Antifungal agent, vasodilator, antihypertensive agent, and anti-inflammatory agent. Phytolaccagenin activates vascular and cardiac Muscarinic receptors, blocks cardiac β-adrenergic receptors, and inhibits voltage-dependent calcium channels in blood vessels. Phytolaccagenin inhibits the growth of Candida albicans and Cryptococcus neoformans. Phytolaccagenin induces vasodilation, produces negative inotropic and negative chronotropic effects, reduces mean arterial pressure, and inhibits LPS-induced inflammation. Phytolaccagenin exhibits enhanced antihypertensive effects in high salt-induced hypertensive rats. Phytolaccagenin can be used in the research of candidiasis, cryptococcosis, hypertension, and inflammation-related diseases.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

純度: 99.86%
CAS 番号: 1802-12-6
分子式: C₃₁H₄₈O₇
分子量:532.71

保管条件:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Phytolaccagenin 構造式

CAS 番号: 1802-12-6

容量

価格（税別）

在庫状況

数量

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性
- 製品説明
- IC₅₀ & Target
- 体外実験
- Parmacokinetics
- 体内実験
化学情報
溶剤 & 溶解度
- 体外
- 体内
純度とドキュメンテーション
参考文献
Help & FAQs
関連分類
Bioz

生物活性

製品説明

IC₅₀ & Target^[2]

β-adrenoceptor

mAChR アイソフォーム固有の製品をすべて表示 :

Adrenergic Receptor アイソフォーム固有の製品をすべて表示 :

Calcium Channel アイソフォーム固有の製品をすべて表示 :

体外実験

Phytolaccagenin inhibits phenylephrine (HY-B0769)- and high K⁺-induced contraction of isolated aortic rings from Sprague-Dawley rats^[2].
Phytolaccagenin (10-100 μM; 30-45 min) inhibits voltage-dependent Ca²⁺ influx in aortic rings isolated from Sprague-Dawley rats, which is confirmed by the rightward shift of the CaCl₂ concentration-response curve^[2].
Phytolaccagenin produces negative inotropic and negative chronotropic effects on isolated spontaneously beating atrial strips from Sprague-Dawley rats, and these effects are partially mediated by cardiac muscarinic receptors and β-adrenergic receptors^[2].
Phytolaccagenin (5-100 μM; 24 h) potently inhibits lipopolysaccharide (LPS)-induced nitric oxide production in RAW 264.7 macrophages, with an IC₅₀ of 18.4 μM, and exhibits stronger activity than Esculentoside A (HY-N0632)^[3].
Phytolaccagenin (60 min) shows no hemolytic activity against red blood cells of New Zealand rabbits, with an HD₅₀ > 500 μM at the maximum concentration of 500 μM^[3].
Phytolaccagenin (100 μM) reduces the viability of RAW 264.7 macrophages^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

RAW 264.7 murine celiac macrophages
Concentration:

100 μM
Incubation Time:

A certain period of time
Result:

Reduced RAW 264.7 macrophage growth by 39%.

Parmacokinetics

Species	Dose	Route	C₀	T_1/2	AUC_0-last	AUC_0-∞	MRT	V_z	CL
Rat^[4]	10 mg/kg	i.v.	8277 ng/mL	1.4 h	5821 ng·h/mL	5999 ng·h/mL	0.9 h	4.2 L/kg	2.1 L/h/kg

体内実験

Phytolaccagenin (0.3-300 µg/kg; i.v.; single dose) produces a dose-dependent antihypertensive effect in high-salt-induced hypertensive SD rats, with a maximum 77.4% fall in MAP at 300 µg/kg i.v., via mechanisms involving muscarinic receptors and nitric oxide pathways^[2].
Phytolaccagenin (0.3-300 µg/kg; i.v.; single dose) produces a dose-dependent hypotensive effect in normotensive anesthetized SD rats, with a maximum 61% fall in MAP at 300 µg/kg i.v^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Sprague-Dawley (SD) (male, 180-250 g, high-salt diet-induced hypertension model)^[2]
Dosage:

0.3 µg/kg; 3 µg/kg; 30 µg/kg; 300 µg/kg
Administration:

i.v.; single dose
Result:

Caused a dose-dependent fall in MAP: 28.4% at 0.3 µg/kg, 50.8% at 3 µg/kg, 68.8% at 30 µg/kg, and 77.4% at 300 µg/kg.
Caused a dose-dependent fall in MAP in atropine-pretreated rats: 7% at 0.3 µg/kg, 27.33% at 3 µg/kg, 63.66% at 30 µg/kg, and 70% at 300 µg/kg.
Caused a dose-dependent fall in MAP in L-NAME-pretreated rats: 34% at 0.3 µg/kg, 51.66% at 3 µg/kg, 57.33% at 30 µg/kg, and 72.33% at 300 µg/kg.
Caused a dose-dependent fall in heart rate: 14.06% at 0.3 µg/kg, 22.53% at 3 µg/kg, 40.67% at 30 µg/kg, and 49.1% at 300 µg/kg.

Animal Model:

Sprague-Dawley (SD) (male, 180-250 g)^[2]
Dosage:

0.3 µg/kg; 3 µg/kg; 30 µg/kg; 300 µg/kg
Administration:

i.v.; single dose
Result:

Caused a dose-dependent fall in MAP: 11% at 0.3 µg/kg, 31.8% at 3 µg/kg, 38.4% at 30 µg/kg, and 61% at 300 µg/kg.
Caused a dose-dependent fall in heart rate: 13.3% at 0.3 µg/kg, 23% at 3 µg/kg, 34.5% at 30 µg/kg, and 53.7% at 300 µg/kg.

化学情報

CAS 番号 1802-12-6
性状 Solid
分子量 532.71
分子式 C₃₁H₄₈O₇
Color White to off-white
SMILES

C[C@@]1(CO)[C@@H](O)[C@@H](O)C[C@]2(C)[C@@]3([H])CC=C4[C@]5([H])C[C@@](C)(C(OC)=O)CC[C@@](C(O)=O)5CC[C@](C)4[C@@](C)3CC[C@@]12[H]
別名

フィトラッカゲニン
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶剤 & 溶解度

体外:

DMSO : 50 mg/mL (93.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8772 mL

9.3860 mL

18.7719 mL

5 mM

0.3754 mL

1.8772 mL

3.7544 mL

10 mM

0.1877 mL

0.9386 mL

1.8772 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (4.69 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (284 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Di Liberto M, et al. Antifungal activity of saponin-rich extracts of Phytolacca dioica and of the sapogenins obtained through hydrolysis. Natural product communications. 2010 Jul;5(7):1013-8. [Content Brief]

[2]. Ul Haq I, et al. Antihypertensive effect and the underlying mechanisms of action of phytolaccagenin in rat models. Clinical and experimental hypertension (New York, N.Y. : 1993). 2022 Aug 18;44(6):557-566. [Content Brief]

[3]. Gong W, et al. Synthesis of novel derivatives of esculentoside A and its aglycone phytolaccagenin, and evaluation of their haemolytic activity and inhibition of lipopolysaccharide-induced nitric oxide production. Chemistry & biodiversity. 2011 Oct;8(10):1833-52. [Content Brief]

[4]. Wei F, et al. Development and validation of a HPLC-MS/MS method for the determination of phytolaccagenin in rat plasma and application to a pharmacokinetic study. Journal of pharmaceutical and biomedical analysis. 2015 Mar 25;107:82-8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8772 mL	9.3860 mL	18.7719 mL	46.9298 mL
	5 mM	0.3754 mL	1.8772 mL	3.7544 mL	9.3860 mL
	10 mM	0.1877 mL	0.9386 mL	1.8772 mL	4.6930 mL
	15 mM	0.1251 mL	0.6257 mL	1.2515 mL	3.1287 mL
	20 mM	0.0939 mL	0.4693 mL	0.9386 mL	2.3465 mL
	25 mM	0.0751 mL	0.3754 mL	0.7509 mL	1.8772 mL
	30 mM	0.0626 mL	0.3129 mL	0.6257 mL	1.5643 mL
	40 mM	0.0469 mL	0.2346 mL	0.4693 mL	1.1732 mL
	50 mM	0.0375 mL	0.1877 mL	0.3754 mL	0.9386 mL
	60 mM	0.0313 mL	0.1564 mL	0.3129 mL	0.7822 mL
	80 mM	0.0235 mL	0.1173 mL	0.2346 mL	0.5866 mL

Keywords

製品名:
製品番号:
数量:
日本の代理先をお選びください:

製品名:
CAS 番号:
生物種:
純度:
数量:

Phytolaccagenin

Anti-Infection Compound Library

GPCR/G Protein Compound Library

Immunology/Inflammation Compound Library

Membrane Transporter/Ion Channel Compound Library

Neuronal Signaling Compound Library

Natural Product Library

Anti-Cardiovascular Disease Compound Library

Antifungal Compound Library

Terpenoids Library

Neurotransmitter Receptor Compound Library

Traditional Chinese Medicine Active Compound Library

Anti-Alzheimer's Disease Compound Library

Neuroprotective Compound Library

Neurodegenerative Disease-related Compound Library

Antidepressant Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Calcium Channel Blocker Library

Antihypertensive Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Ion Channel Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mongolian Medicine Compound Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

mAChR アイソフォーム固有の製品をすべて表示

Adrenergic Receptor アイソフォーム固有の製品をすべて表示

Calcium Channel アイソフォーム固有の製品をすべて表示

生物活性

化学情報

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

Phytolaccagenin 関連分類

HY-N1433 関連分類