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Cat. No.: HY-P0248F1

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Kemptide-FITC

Kemptide-FITC is a fluorescently labeled Kemptide (HY-P0248). Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA). Kemptide-FITC enables the quantification of PKA activity and can also indirectly determine cAMP concentrations through the activation of PKA in sperm insoluble fractions. Kemptide-FITC is applicable for kinase mobility shift assay (KiMSA), and its fluorescent signal shows a linear response with increasing peptide amount within a specific range on agarose gels.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

화학식: C₅₉H₈₄N₁₆O₁₅S
분자량:1289.46

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

맞춤형 펩타이드 합성

Kemptide-FITC Chemical Structure

Amount: USD 0.00

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- In Vitro
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

All PKA Isoforms :

In Vitro

Kemptide-FITC serves as a non-radioactive substrate for KiMSA, enabling quantitative detection of the PKA activity of recombinant PKA-C. Its phosphorylated and non-phosphorylated forms can be clearly separated via agarose gel electrophoresis, with no spectral drift interfering with fluorescence quantitative analysis^[1].
Kemptide-FITC (non-phosphorylated) exhibits a linear fluorescence signal response in the range of 50 to 300 pmoles when separated by agarose gel electrophoresis^[2].
Kemptide-FITC (30 μM; 25 min at 37°C) serves as a substrate for the recombinant catalytic subunit of protein kinase A (PKA) α. Detection via KiMSA reveals a linear relationship between the enzyme concentration (53-533 ng/mL) and the phosphorylation level of Kemptide-FITC^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

분자량 1289.46
화학식 C₅₉H₈₄N₁₆O₁₅S
Sequence

Leu-Arg-Arg-Ala-Ser-Leu-Gly-Lys-FITC
Sequence Shortening

LRRASLGK-FITC
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Handling Instructions (2659 KB)

References

[1]. Novero AG, et al. Kinase Mobility Shift Assay (KiMSA) for Assessing Protein Kinase A Activity. Bio-protocol. 2025 Jul 05;15(13):e5366.

[2]. Novero AG, et al. A versatile kinase mobility shift assay (KiMSA) for PKA analysis and cyclic AMP detection in sperm physiology (and beyond). Frontiers in cell and developmental biology. 2024;12:1356566.

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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