Kemptide-FITC
Kemptide-FITC is a fluorescently labeled Kemptide (HY-P0248). Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA). Kemptide-FITC enables the quantification of PKA activity and can also indirectly determine cAMP concentrations through the activation of PKA in sperm insoluble fractions. Kemptide-FITC is applicable for kinase mobility shift assay (KiMSA), and its fluorescent signal shows a linear response with increasing peptide amount within a specific range on agarose gels.
For research use only. We do not sell to patients.
- Formula: C59H84N16O15S
- Molecular Weight:1289.46
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Kemptide-FITC serves as a non-radioactive substrate for KiMSA, enabling quantitative detection of the PKA activity of recombinant PKA-C. Its phosphorylated and non-phosphorylated forms can be clearly separated via agarose gel electrophoresis, with no spectral drift interfering with fluorescence quantitative analysis[1].
Kemptide-FITC (non-phosphorylated) exhibits a linear fluorescence signal response in the range of 50 to 300 pmoles when separated by agarose gel electrophoresis[2].
Kemptide-FITC (30 μM; 25 min at 37°C) serves as a substrate for the recombinant catalytic subunit of protein kinase A (PKA) α. Detection via KiMSA reveals a linear relationship between the enzyme concentration (53-533 ng/mL) and the phosphorylation level of Kemptide-FITC[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Molecular Weight 1289.46
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Formula C59H84N16O15S
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Sequence
Leu-Arg-Arg-Ala-Ser-Leu-Gly-Lys-FITC
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Sequence Shortening
LRRASLGK-FITC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Kemptide-FITC Related Classifications
HY-P0248F1 Related Classifications
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