Kemptide-FITC
Kemptide-FITC is a fluorescently labeled Kemptide (HY-P0248). Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA). Kemptide-FITC enables the quantification of PKA activity and can also indirectly determine cAMP concentrations through the activation of PKA in sperm insoluble fractions. Kemptide-FITC is applicable for kinase mobility shift assay (KiMSA), and its fluorescent signal shows a linear response with increasing peptide amount within a specific range on agarose gels.
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- Formule: C59H84N16O15S
- Masse moléculaire:1289.46
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
Kemptide-FITC (non-phosphorylated) exhibits a linear fluorescence signal response in the range of 50 to 300 pmoles when separated by agarose gel electrophoresis[2].
Kemptide-FITC (30 μM; 25 min at 37°C) serves as a substrate for the recombinant catalytic subunit of protein kinase A (PKA) α. Detection via KiMSA reveals a linear relationship between the enzyme concentration (53-533 ng/mL) and the phosphorylation level of Kemptide-FITC[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Masse moléculaire 1289.46
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Formule C59H84N16O15S
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Sequence
Leu-Arg-Arg-Ala-Ser-Leu-Gly-Lys-FITC
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Sequence Shortening
LRRASLGK-FITC
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Kemptide-FITC Related Classifications
HY-P0248F1 Related Classifications
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Keywords
- Kemptide-FITC
- Biochemical Assay Reagents
- Drug Derivative
- PKA
- Drug derivative
- Protein kinase A
- cAMP-dependent protein kinase
- recombinant PKA catalytic subunit alpha
- recombinant PKA-C
- KiMSA
- Kinase Mobility Shift Assay
- sperm insoluble fractions
- agarose gel electrophoresis
- mouse sperm extracts
- Inhibitor
- inhibitor
- inhibit