1. Protein Tyrosine Kinase/RTK
    Stem Cell/Wnt
  2. PKA

Kemptide (Synonyms: Leu-Arg-Arg-Ala-Ser-Leu-Gly; LRRASLG)

Cat. No.: HY-P0248
Handling Instructions

Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA).

For research use only. We do not sell to patients.
Kemptide Chemical Structure

Kemptide Chemical Structure

CAS No. : 65189-71-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO $161 In-stock
1 mg $50 In-stock
5 mg $110 In-stock
10 mg $190 In-stock
25 mg $390 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

Other Forms of Kemptide:

  • Biological Activity

  • Protocol

  • Technical Information

  • References

Description

Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA).

In Vitro

Kemptide is a synthetic construct of a substrate for cAMP-dependent protein kinase (PK). Different types of intact cells catalyze the phosphorylation of Kemptide in the presence of extracellular ATP and cAMP with Km values of 3-4 uM for Kemptide[1]. Kemptide is a synthetic peptide substrate derived from pyruvate kinase[2].

References
Preparing Stock Solutions
Concentration Volume (DMSO) Mass 1 mg 5 mg 10 mg
1 mM 1.2955 mL 6.4774 mL 12.9549 mL
5 mM 0.2591 mL 1.2955 mL 2.5910 mL
10 mM 0.1295 mL 0.6477 mL 1.2955 mL
Kinase Assay
[2]

PKA activity is measured in pools of 10 oocytes at various stages of maturation using the SignaTECT cAMP-Dependent Protein Kinase (PKA) Assay System. Measurements of basal PKA activity are performed in the absence of exogenous cAMP, whereas measurements of total (cAMP-stimulated) PKA activity are performed in reaction buffer containing 0.025 mM cAMP[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

771.91

Formula

C₃₂H₆₁N₁₃O₉

CAS No.

65189-71-1

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number

 

Organization name *

Country *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Kemptide
Cat. No.:
HY-P0248
Quantity: