1. Protein Tyrosine Kinase/RTK
    Stem Cell/Wnt
  2. PKA

Kemptide (Synonyms: Leu-Arg-Arg-Ala-Ser-Leu-Gly; LRRASLG)

Cat. No.: HY-P0248 Purity: >98.0%
Handling Instructions

Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA).

For research use only. We do not sell to patients.
Kemptide Chemical Structure

Kemptide Chemical Structure

CAS No. : 65189-71-1

Size Price Stock Quantity
10 mM * 1 mL in Water USD 194 In-stock
1 mg USD 60 In-stock
5 mg USD 132 In-stock
10 mg USD 228 In-stock
25 mg USD 468 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA).

In Vitro

Kemptide is a synthetic construct of a substrate for cAMP-dependent protein kinase (PK). Different types of intact cells catalyze the phosphorylation of Kemptide in the presence of extracellular ATP and cAMP with Km values of 3-4 uM for Kemptide[1]. Kemptide is a synthetic peptide substrate derived from pyruvate kinase[2].

Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.2955 mL 6.4774 mL 12.9549 mL
5 mM 0.2591 mL 1.2955 mL 2.5910 mL
10 mM 0.1295 mL 0.6477 mL 1.2955 mL
Please refer to the solubility information to select the appropriate solvent.
Kinase Assay

PKA activity is measured in pools of 10 oocytes at various stages of maturation using the SignaTECT cAMP-Dependent Protein Kinase (PKA) Assay System. Measurements of basal PKA activity are performed in the absence of exogenous cAMP, whereas measurements of total (cAMP-stimulated) PKA activity are performed in reaction buffer containing 0.025 mM cAMP[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight








Powder -80°C 2 years
  -20°C 1 year
In solvent -80°C 6 months
  -20°C 1 month

Room temperature in continental US; may vary elsewhere

Solvent & Solubility


* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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