L-Leucyl-4-nitroanilide
Based on 1 Customer Validation
L-Leucyl-4-nitroanilide is a chromogenic substrate suitable for activity assays of leucine aminopeptidase, and it can be used in studies related to the enzymatic properties of aminopeptidases.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 4178-93-2
- Formula: C12H17N3O3
- Molecular Weight:251.29
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Storage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
L-Leucyl-4-nitroanilide (LpNa) serves as a substrate for extracellular leucine aminopeptidases 1 and 2 derived from Aspergillus oryzae (I.P.410). These two enzymes exhibit arylamidase activity toward LpNa, and this activity is inhibited by straight-chain alcohols (but not branched-chain alcohols) in a carbon chain length-dependent manner[1].
L-Leucyl-4-nitroanilide (Leu-4-NA) (25 mM; 2 h) is cleaved by purified aminopeptidase from Brevibacterium linens ATCC 9174, generating a colorimetric signal at 405 nm[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 4178-93-2
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Appearance Solid
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Molecular Weight 251.29
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Formula C12H17N3O3
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Color Light yellow to yellow
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SMILES
N[C@@H](CC(C)C)C(NC1=CC=C([N+]([O-])=O)C=C1)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 100 mg/mL (397.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9795 mL | 19.8973 mL | 39.7947 mL | 99.4867 mL |
| 5 mM | 0.7959 mL | 3.9795 mL | 7.9589 mL | 19.8973 mL | |
| 10 mM | 0.3979 mL | 1.9897 mL | 3.9795 mL | 9.9487 mL | |
| 15 mM | 0.2653 mL | 1.3265 mL | 2.6530 mL | 6.6324 mL | |
| 20 mM | 0.1990 mL | 0.9949 mL | 1.9897 mL | 4.9743 mL | |
| 25 mM | 0.1592 mL | 0.7959 mL | 1.5918 mL | 3.9795 mL | |
| 30 mM | 0.1326 mL | 0.6632 mL | 1.3265 mL | 3.3162 mL | |
| 40 mM | 0.0995 mL | 0.4974 mL | 0.9949 mL | 2.4872 mL | |
| 50 mM | 0.0796 mL | 0.3979 mL | 0.7959 mL | 1.9897 mL | |
| 60 mM | 0.0663 mL | 0.3316 mL | 0.6632 mL | 1.6581 mL | |
| 80 mM | 0.0497 mL | 0.2487 mL | 0.4974 mL | 1.2436 mL | |
| 100 mM | 0.0398 mL | 0.1990 mL | 0.3979 mL | 0.9949 mL |
L-Leucyl-4-nitroanilide Related Classifications
HY-W011911 Related Classifications
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- L-Leucyl-4-nitroanilide
- 4178-93-2
- Biochemical Assay Reagents
- Aminopeptidase
- Brevibacterium linens ATCC 9174
- Leucine Aminopeptidase 1
- arylamidase activity
- extracellular leucine aminopeptidase I
- Leucine Aminopeptidase 2
- Cd2+
- cobalt
- Aspergillus oryzae
- extracellular leucine aminopeptidase II
- Brevibacterium linens aminopeptidase
- Inhibitor
- inhibitor
- inhibit