L-Leucyl-4-nitroanilide hydrochloride
Based on 1 Customer Validation
L-Leucyl-4-nitroanilide hydrochloride is a chromogenic substrate suitable for activity assays of leucine aminopeptidase, and it can be used in studies related to the enzymatic properties of aminopeptidases.
For research use only. We do not sell to patients.
- Purity: 99.97%
- CAS No.: 16010-98-3
- Formula: C12H18ClN3O3
- Molecular Weight:287.75
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
L-Leucyl-4-nitroanilide (LpNa) hydrochloride serves as a substrate for extracellular leucine aminopeptidases 1 and 2 derived from Aspergillus oryzae (I.P.410). These two enzymes exhibit arylamidase activity toward LpNa, and this activity is inhibited by straight-chain alcohols (but not branched-chain alcohols) in a carbon chain length-dependent manner[1].
L-Leucyl-4-nitroanilide (Leu-4-NA) (25 mM; 2 h) hydrochloride is cleaved by purified aminopeptidase from Brevibacterium linens ATCC 9174, generating a colorimetric signal at 405 nm[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 16010-98-3
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Appearance Solid
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Molecular Weight 287.75
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Formula C12H18ClN3O3
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Color Off-white to light yellow
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SMILES
N[C@@H](CC(C)C)C(NC1=CC=C([N+]([O-])=O)C=C1)=O.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (347.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4752 mL | 17.3762 mL | 34.7524 mL | 86.8810 mL |
| 5 mM | 0.6950 mL | 3.4752 mL | 6.9505 mL | 17.3762 mL | |
| 10 mM | 0.3475 mL | 1.7376 mL | 3.4752 mL | 8.6881 mL | |
| 15 mM | 0.2317 mL | 1.1584 mL | 2.3168 mL | 5.7921 mL | |
| 20 mM | 0.1738 mL | 0.8688 mL | 1.7376 mL | 4.3440 mL | |
| 25 mM | 0.1390 mL | 0.6950 mL | 1.3901 mL | 3.4752 mL | |
| 30 mM | 0.1158 mL | 0.5792 mL | 1.1584 mL | 2.8960 mL | |
| 40 mM | 0.0869 mL | 0.4344 mL | 0.8688 mL | 2.1720 mL | |
| 50 mM | 0.0695 mL | 0.3475 mL | 0.6950 mL | 1.7376 mL | |
| 60 mM | 0.0579 mL | 0.2896 mL | 0.5792 mL | 1.4480 mL | |
| 80 mM | 0.0434 mL | 0.2172 mL | 0.4344 mL | 1.0860 mL | |
| 100 mM | 0.0348 mL | 0.1738 mL | 0.3475 mL | 0.8688 mL |
L-Leucyl-4-nitroanilide hydrochloride Related Classifications
HY-137872 Related Classifications
Powered by Bioz
- L-Leucyl-4-nitroanilide
- 16010-98-3
- Biochemical Assay Reagents
- Aminopeptidase
- Brevibacterium linens ATCC 9174
- Leucine Aminopeptidase 1
- arylamidase activity
- extracellular leucine aminopeptidase I
- Leucine Aminopeptidase 2
- Cd2+
- cobalt
- Aspergillus oryzae
- extracellular leucine aminopeptidase II
- Brevibacterium linens aminopeptidase
- Inhibitor
- inhibitor
- inhibit