L-γ-Glutamyl-p-nitroanilide
Based on 7 publication(s) in Google Scholar
L-γ-Glutamyl-p-nitroanilide Monohydrate is a glutamic acid derivative.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 7300-59-6
- Formula: C11H13N3O5
- Molecular Weight:267.24
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications Citing Use of MedChemExpress (MCE) L-γ-Glutamyl-p-nitroanilide
More- Adv Sci (Weinh). 2025 Aug 10:e07057. [Abstract]
- Antioxidants (Basel). 2021 Aug 25;10(9):1349. [Abstract]
- Biochem Pharmacol. 2023 Oct:216:115767. [Abstract]
- Int Immunopharmacol. 2023 Jun:119:110216. [Abstract]
- Front Pharmacol. 2022 Feb 23;13:816133. [Abstract]
- Clin Respir J. 2024 Aug;18(8):e13825. [Abstract]
- Tohoku J Exp Med. 2024 Nov 14;264(1):31-39. [Abstract]
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Others
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Cell Proliferation/Viability Assay
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WB
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Others
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WB
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| C6 | IC50 |
70.2 μM
Compound: 1; GPNA
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Inhibition of ASCT2-mediated glutamine transport in rat C6 cells using [3H]-glutamine after 15 mins by scintillation counting
Inhibition of ASCT2-mediated glutamine transport in rat C6 cells using [3H]-glutamine after 15 mins by scintillation counting
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[PMID: 26750251] |
| HEK293 | IC50 |
1.2 mM
Compound: 1; GPNA
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Inhibition of ASCT2-mediated glutamine transport in human HEK293 cells using [3H]-glutamine after 15 mins by scintillation counting
Inhibition of ASCT2-mediated glutamine transport in human HEK293 cells using [3H]-glutamine after 15 mins by scintillation counting
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[PMID: 26750251] |
| HEK293 | IC50 |
954 μM
Compound: 1
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Inhibition of ASCT2-mediated [3H]glutamine uptake in HEK293 cells after 15 mins by Live-cell glutamine uptake assay
Inhibition of ASCT2-mediated [3H]glutamine uptake in HEK293 cells after 15 mins by Live-cell glutamine uptake assay
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[PMID: 25435145] |
Amino acids and amino acid derivatives have been commercially used as ergogenic supplements. They influence the secretion of anabolic hormones, supply of fuel during exercise, mental performance during stress related tasks and prevent exercise induced muscle damage. They are recognized to be beneficial as ergogenic dietary substances[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 7300-59-6
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Appearance Solid
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Molecular Weight 267.24
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Formula C11H13N3O5
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Color White to yellow
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (7)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Real-Time Tracking of ASCT2-Mediated Glutamine Uptake in Living Tumors With a Bioorthogonal Bioluminescent Probe. [Abstract]2025 Aug 10:e07057. PMID: 40783801
L-γ-Glutamyl-p-nitroanilide purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 10:e07057. [Abstract]
Changes in blue light intensity over time in cells pretreated with different inhibitors using GPNA hydrochloride (250/500 µM).
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Antioxidants (Basel)
Glutamine Modulates Expression and Function of Glucose 6-Phosphate Dehydrogenase via NRF2 in Colon Cancer Cells. [Abstract]2021 Aug 25;10(9):1349. PMID: 34572981
L-γ-Glutamyl-p-nitroanilide purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2021 Aug 25;10(9):1349. [Abstract]
The expression level of NRF2 protein was detected after treatment with BPTES (10 µM), CB-839 (5 µM), R162 (20 µM) and GPNA hydrochloride (100 µM) for 24 hours.
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Biochem Pharmacol
ASCT2-mediated glutamine uptake promotes Th1 differentiation via ROS-EGR1-PAC1 pathway in oral lichen planus. [Abstract]2023 Oct:216:115767. PMID: 37634599 -
Int Immunopharmacol
ASCT2-mediated glutamine uptake of epithelial cells facilitates CCL5-induced T cell infiltration via ROS-STAT3 pathway in oral lichen planus. [Abstract]2023 Jun:119:110216. PMID: 37116342 -
Front Pharmacol
Ca2+-Permeable Channels/Ca2+ Signaling in the Regulation of Ileal Na+/Gln Co-Transport in Mice. [Abstract]2022 Feb 23;13:816133. PMID: 35281933
L-γ-Glutamyl-p-nitroanilide purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2022 Feb 23;13:816133. [Abstract]
Representative time progression of Isc peaks stimulated by Gln (5 mM) with or without GPNA hydrochloride (200 μM) on the ileal mucosa.
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Clin Respir J
TFAP2A Activates S100A2 to Mediate Glutamine Metabolism and Promote Lung Adenocarcinoma Metastasis. [Abstract]2024 Aug;18(8):e13825. PMID: 39187936
L-γ-Glutamyl-p-nitroanilide purchased from MedChemExpress. Usage Cited in: Clin Respir J. 2024 Aug;18(8):e13825. [Abstract]
GPNA hydrochloride (2 mM).Cell viability detection.
L-γ-Glutamyl-p-nitroanilide purchased from MedChemExpress. Usage Cited in: Clin Respir J. 2024 Aug;18(8):e13825. [Abstract]
GPNA hydrochloride (2 mM).MMP‐2 and MMP‐9 expression detection.
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Tohoku J Exp Med
HJURP Derived from Cancer-Associated Fibroblasts Promotes Glutamine Metabolism to Induce Resistance to Doxorubicin in Ovarian Cancer. [Abstract]2024 Nov 14;264(1):31-39. PMID: 38866531
Solvent & Solubility
DMSO : 12.5 mg/mL (46.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7420 mL | 18.7098 mL | 37.4195 mL | 93.5489 mL |
| 5 mM | 0.7484 mL | 3.7420 mL | 7.4839 mL | 18.7098 mL | |
| 10 mM | 0.3742 mL | 1.8710 mL | 3.7420 mL | 9.3549 mL | |
| 15 mM | 0.2495 mL | 1.2473 mL | 2.4946 mL | 6.2366 mL | |
| 20 mM | 0.1871 mL | 0.9355 mL | 1.8710 mL | 4.6774 mL | |
| 25 mM | 0.1497 mL | 0.7484 mL | 1.4968 mL | 3.7420 mL | |
| 30 mM | 0.1247 mL | 0.6237 mL | 1.2473 mL | 3.1183 mL | |
| 40 mM | 0.0935 mL | 0.4677 mL | 0.9355 mL | 2.3387 mL |