1. Metabolic Enzyme/Protease
    Apoptosis
    Anti-infection
  2. Hexokinase
    Mitochondrial Metabolism
    Apoptosis
    Parasite
  3. Lonidamine

Lonidamine  (Synonyms: AF-1890; Diclondazolic Acid; DICA)

Cat. No.: HY-B0486 Purity: 99.44%
COA Handling Instructions

Lonidamine (AF-1890) is a hexokinase and mitochondrial pyruvate carrier inhibitor (Ki: 2.5 μM). Lonidamine also inhibits aerobic glycolysis in cancer cells. Lonidamine can be used in the research of mitochondrial metabolism and inflammation, such as pulmonary fibrosis.

For research use only. We do not sell to patients.

Lonidamine Chemical Structure

Lonidamine Chemical Structure

CAS No. : 50264-69-2

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 73 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 66 In-stock
Estimated Time of Arrival: December 31
10 mg USD 79 In-stock
Estimated Time of Arrival: December 31
50 mg USD 158 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 6 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Lonidamine purchased from MCE. Usage Cited in: Cell Metab. 2022 Nov 11;S1550-4131(22)00490-9.  [Abstract]

    Combination treatment with Rimegepant and Lonidamine, Anlotinib, or Bevacizumab lead to a reduced proportion of Ki67+ cancer cells, reduce LC3B puncta, and the accumulation of p62 puncta in cancer cells, indicating impaired cell proliferation and autophagic flux.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Lonidamine (AF-1890) is a hexokinase and mitochondrial pyruvate carrier inhibitor (Ki: 2.5 μM). Lonidamine also inhibits aerobic glycolysis in cancer cells. Lonidamine can be used in the research of mitochondrial metabolism and inflammation, such as pulmonary fibrosis[1][2][3].

    IC50 & Target

    Ki: 2.5 μM (Mitochondrial pyruvate carrier)[2]

    In Vitro

    Lonidamine (100 μM, 24 h) inhibits TGF-β-stimulated lactate production and oxygen consumption rate in AKR-2B and TIG-1 cells[3].
    Lonidamine (100 μM, 24/48 h) inhibits H2030BrM3 and A549 cell proliferation[4].
    Lonidamine (100-200 μM, 24 h) inhibits H2030BrM3 and A549 cell invasion[4].
    Lonidamine (100-1000 μM, 24 h) inhibits mitochondrial complex I and II activities[4].
    Lonidamine (200 μM, 24 h) increases ROS generation in H2030BrM3 lung cancer cells[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Lonidamine (oral administration, 10-100 mg/kg/day, d10 to d20) improves lung function by inhibiting hexokinase 2 (HK2) activity in BLM-induced pulmonary fibrosis murine model[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Lonidamine (oral administration, 10-100 mg/kg/day, d10 to d20) improves lung function by inhibiting hexokinase 2 (HK2) activity in BLM-induced pulmonary fibrosis murine model[3].
    Dosage: 10, 30, 100 mg/kg/day
    Administration: Oral administration, daily, d10 to d20 after BLM treatment.
    Result: Partially or completely reversed the increases in HK2 and lactate induced by BLM and reduced the expression of 10 profibrotic mediators.
    Clinical Trial
    Molecular Weight

    321.16

    Formula

    C15H10Cl2N2O2

    CAS No.
    SMILES

    O=C(C1=NN(CC2=CC=C(Cl)C=C2Cl)C3=C1C=CC=C3)O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (155.69 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1137 mL 15.5686 mL 31.1371 mL
    5 mM 0.6227 mL 3.1137 mL 6.2274 mL
    10 mM 0.3114 mL 1.5569 mL 3.1137 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (6.48 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (6.48 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.44%

    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Lonidamine
    Cat. No.:
    HY-B0486
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