1. NF-κB
    Metabolic Enzyme/Protease
    Immunology/Inflammation
    Apoptosis
    Protein Tyrosine Kinase/RTK
  2. NF-κB
    Reactive Oxygen Species
    Apoptosis
    VEGFR
    c-Myc
  3. Mollugin

Mollugin 

Cat. No.: HY-N0316 Purity: 99.48%
Handling Instructions

Mollugin is an orally active and potent NF-κB inhibitor. Mollugin induces S-phase arrest of HepG2 cells, and increased intracellular reactive oxygen species (ROS) levels. Mollugin induces DNA damage in HepG2 cells, as well as an increase in the expression of p-H2AX. Mollugin shows anti-cancer effect by inhibiting TNF-α-induced NF-κB activation. Mollugin enhances the osteogenic action of BMP-2 (bone morphogenetic protein 2) via the p38-Smad signaling pathway.

For research use only. We do not sell to patients.

Mollugin Chemical Structure

Mollugin Chemical Structure

CAS No. : 55481-88-4

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5 mg USD 50 In-stock
Estimated Time of Arrival: December 31
10 mg USD 90 In-stock
Estimated Time of Arrival: December 31
20 mg USD 150 In-stock
Estimated Time of Arrival: December 31
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Description

Mollugin is an orally active and potent NF-κB inhibitor. Mollugin induces S-phase arrest of HepG2 cells, and increased intracellular reactive oxygen species (ROS) levels. Mollugin induces DNA damage in HepG2 cells, as well as an increase in the expression of p-H2AX. Mollugin shows anti-cancer effect by inhibiting TNF-α-induced NF-κB activation. Mollugin enhances the osteogenic action of BMP-2 (bone morphogenetic protein 2) via the p38-Smad signaling pathway[1][2][3].

In Vitro

Mollugin (0-80 μM, 24 h) inhibits the expression of an NF-κB reporter gene induced by TNF-α in a dose-dependent manner[1].
Mollugin (0-80 μM, 12 h) inhibits the proliferation of HeLa cells[1].
Mollugin (0-80 μM, 12 h) inhibits TNF-α-induced phosphorylation and nuclear translocation of p65, phosphorylation and degradation of IκBα, and IKK phosphorylation, and inhibits the TNF-α-induced mRNA expression of Cyclin D1, c-Myc, and VEGF[1].
Mollugin (0-80 μM, 12 h) enhances TNF-α-induced apoptosis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: HeLa, Hep3B, and HEK293 cells
Concentration: 0, 10, 20, 40, and 80 μM
Incubation Time: 24 h
Result: Significantly inhibited NF-κB reporter gene expression in a dose-dependent manner, did not display significant cellular toxicity in HeLa, Hep3B, and HEK293 cells.

Cell Proliferation Assay[1]

Cell Line: HeLa cells
Concentration: 0, 20, 40, and 80 μM
Incubation Time: 12 h
Result: Inhibited the proliferation of HeLa cells.

Apoptosis Analysis[1]

Cell Line: HeLa cells
Concentration: 0, 10, 20, 40, and 80 μM
Incubation Time: 12 h
Result: Slightly affected the caspase-3 activation, potentiated the effect of TNF-α-induced PARP cleavage, and enhanced the apoptotic effects of TNF-α.

Western Blot Analysis[1]

Cell Line: HeLa cells
Concentration: 0, 10, 20, 40, and 80 μM
Incubation Time: 12 h
Result: Significantly inhibited the TNF-α-induced p65 phosphorylation and block TNF-α-induced nuclear translocation of p65 in a dose-dependent manner, completely inhibited degradation of IκBα at 80 μM, and abolished the TNF-α-induced IKK phosphorylation at 80 μM. Inhibited the TNF-α-induced mRNA expression of Cyclin D1, c-Myc, and VEGF at 80 μM.
In Vivo

Mollugin (0-75 mg/kg, Orally, three times per week for 36 days) inhibits growth of HeLa cells in a xenograft tumor model[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c female athymic nude mice (six-week-old, subcutaneously injected with 0.2 mL HeLa cells)[1]
Dosage: 0, 25 and 75 mg/kg
Administration: Orally, three times per week for 36 days
Result: Suppressed tumor growth, whereas the body weight did not change. Significantly reduced the protein expression of p-p65 and COX-2 in the tumors.
Molecular Weight

284.31

Formula

C17H16O4

CAS No.
SMILES

O=C(C1=C(C=CC(C)(C)O2)C2=C3C=CC=CC3=C1O)OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (117.23 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5173 mL 17.5864 mL 35.1729 mL
5 mM 0.7035 mL 3.5173 mL 7.0346 mL
10 mM 0.3517 mL 1.7586 mL 3.5173 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution

*All of the co-solvents are available by MCE.
References

Purity: 99.48%

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