N-Nitrosodiethanolamine-d8
Based on 1 Customer Validation
N-Nitrosodiethanolamine-d8 is the deuterium labeled N-Nitrosodiethanolamine.
For research use only. We do not sell to patients.
- Purity: 98.33%
- CAS No.: 1173019-53-8
- Formula: C4H2D8N2O3
- Molecular Weight:142.18
-
Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
-
CAS No. 1173019-53-8
-
Unlabeled Cas 1116-54-7
-
Appearance Liquid
-
Molecular Weight 142.18
-
Formula C4H2D8N2O3
-
Color Colorless to light yellow
-
SMILES
OC([2H])([2H])C([2H])([2H])N(N=O)C([2H])([2H])C([2H])([2H])O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (703.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (17.58 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (17.58 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (631 KB)
- English - EN (631 KB)
- Français - FR (631 KB)
- Deutsch - DE (631 KB)
- Norwegian - NO (631 KB)
- Español - ES (631 KB)
- Swedish - SV (631 KB)
- Italian - IT (631 KB)
- Korean - KR (631 KB)
- Portuguese - PT (631 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 7.0333 mL | 35.1667 mL | 70.3334 mL | 175.8335 mL |
| 5 mM | 1.4067 mL | 7.0333 mL | 14.0667 mL | 35.1667 mL | |
| 10 mM | 0.7033 mL | 3.5167 mL | 7.0333 mL | 17.5833 mL | |
| 15 mM | 0.4689 mL | 2.3444 mL | 4.6889 mL | 11.7222 mL | |
| 20 mM | 0.3517 mL | 1.7583 mL | 3.5167 mL | 8.7917 mL | |
| 25 mM | 0.2813 mL | 1.4067 mL | 2.8133 mL | 7.0333 mL | |
| 30 mM | 0.2344 mL | 1.1722 mL | 2.3444 mL | 5.8611 mL | |
| 40 mM | 0.1758 mL | 0.8792 mL | 1.7583 mL | 4.3958 mL | |
| 50 mM | 0.1407 mL | 0.7033 mL | 1.4067 mL | 3.5167 mL | |
| 60 mM | 0.1172 mL | 0.5861 mL | 1.1722 mL | 2.9306 mL | |
| 80 mM | 0.0879 mL | 0.4396 mL | 0.8792 mL | 2.1979 mL | |
| 100 mM | 0.0703 mL | 0.3517 mL | 0.7033 mL | 1.7583 mL |
N-Nitrosodiethanolamine-d8 Related Classifications
HY-132787S Related Classifications
Powered by Bioz