2. Antibody-drug Conjugate/ADC Related
  3. Peptide-Drug Conjugates (PDCs)
  4. 2-octynoic acid-BSA

NHS-activated 2-octynoic acid-BSA is an immunogen used to establish a mouse model of primary biliary cholangitis (PBC). When used in combination with Complete Freund's Adjuvant (HY-153808), NHS-activated 2-octynoic acid-BSA stimulates the body's immune system, which recognizes it as a foreign antigen and triggers an immune response against autologous biliary epithelial cells. This leads to the production of anti-PDC-E2 antibodies, causing bile duct damage and inflammation. NHS-activated 2-octynoic acid-BSA is promising for use in primary biliary cholangitis research.

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2-octynoic acid-BSA

2-octynoic acid-BSA Chemical Structure

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2-octynoic acid-BSA Related Antibodies

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Description

NHS-activated 2-octynoic acid-BSA is an immunogen used to establish a mouse model of primary biliary cholangitis (PBC). When used in combination with Complete Freund's Adjuvant (HY-153808), NHS-activated 2-octynoic acid-BSA stimulates the body's immune system, which recognizes it as a foreign antigen and triggers an immune response against autologous biliary epithelial cells. This leads to the production of anti-PDC-E2 antibodies, causing bile duct damage and inflammation. NHS-activated 2-octynoic acid-BSA is promising for use in primary biliary cholangitis research[1].

In Vitro

2-octynoic acid (20 min (0°C stirring); overnight (ambient temp warming); 3 h (BSA coupling)) is successfully conjugated to BSA via lysine residues following a multi-step chemical synthesis and purification workflow[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2-octynoic acid-BSA Related Antibodies
In Vivo

2-octynoic acid-BSA (100 μg per mouse; i.p.; initial in Complete Freund’s Adjuvant, boosts every 2 weeks in Incomplete Freund’s Adjuvant) induces PBC-like autoimmune cholangitis in female C57BL/6J mice, including 100% seroconversion to anti-PDC-E2 antibodies by 8 weeks, elevated Th1 cytokines, liver lymphoid infiltration, and bile duct lesions consistent with human PBC[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J (female, 8 to 9 weeks of age, immunization-induced PBC-like autoimmune cholangitis)[1]
Dosage: 100 μg per mouse
Administration: i.p.; initial in Complete Freund’s Adjuvant, boosts every 2 weeks in Incomplete Freund’s Adjuvant; up to 24 weeks
Result: Induced PDC-E2-reactive antibodies in 100% (13/13) of immunized mice by 8 weeks post-immunization.
Elevated serum IgG (OD: 0.33) and IgM (OD: 0.08) anti-PDC-E2 levels at 4 weeks post-immunization.
Elevated serum IgG (OD: 1.23), IgA (OD: 0.55), and IgM (OD: 0.27) anti-PDC-E2 levels at 8 weeks post-immunization.
Elevated serum TNF-α (79.0 pg/mL) and IFN-γ (14.7 pg/mL) levels at 4 weeks post-immunization, with elevation sustained through 12 weeks.
Caused mild portal lymphoid cell infiltrates, mild chronic nonsuppurative destructive cholangitis-like bile duct lesions, ductopenia, scattered epithelioid granulomas, and small areas of mild focal necrosis in all tested mice at 12 and 24 weeks post-immunization.
Decreased liver CD4/CD8 ratio to 2.03 at 12 weeks post-immunization compared to controls.
Increased total liver lymphoid cell number to at 12 weeks post-immunization.
Increased absolute numbers of liver CD8+ T cells and CD19+ B cells at 12 weeks post-immunization.
Increased frequency of liver CD8+ T cells co-expressing CD44 at 12 weeks post-immunization.
Molecular Weight

69068 (average)

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[2-octynoic acid-BSA]
Shipping

Shipping with dry ice.
Storage

-80°C, protect from light
Purity & Documentation
References
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2-octynoic acid-BSA Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-octynoic acid-BSAPeptide-Drug Conjugates (PDCs)PDC-E2IFN-γCD44CD8+ T cellsautoimmune cholangitisTNF-αIgAprimary biliary cirrhosisIgGIgMInhibitorinhibitorinhibit

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