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Art. -Nr.: HY-183296

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NLRP3-IN-91

NLRP3-IN-91 is a potent NLRP3 inflammasome inhibitor with a K_d of 558.4 nM. NLRP3-IN-91 directly targets the NLRP3 NACHT domain, blocks inflammasome assembly and activation, and exerts anti-inflammatory effects. NLRP3-IN-91 increases survival time in a murine model of LPS (HY-D1056)-induced sepsis. NLRP3-IN-91 can be used for the research of sepsis.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Formel: C₂₇H₂₇ClFN₃O₆S
Molecular Weight:576.04

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

NLRP3-IN-91 Chemical Structure

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

NLRP3 inflammasome

558.4 nM (Kd)

IL-1β

0.094 μM (IC₅₀)

Caspase-1

Alle NOD-like Receptor (NLR) Isoform-spezifische Produkte anzeigen :

Alle Interleukin Related Isoform-spezifische Produkte anzeigen :

Alle Caspase Isoform-spezifische Produkte anzeigen :

In Vitro

NLRP3-IN-91 (compound D12) (0.04-10 μM; 30 min) potently inhibits NLRP3 inflammasome-mediated IL-1β secretion in J774A.1 murine macrophages with an IC₅₀ of 0.094 μM^[1].
NLRP3-IN-91 (24 h) shows low cytotoxicity in vitro, with CC₅₀ values >30 μM in J774A.1 murine macrophages and >100 μM in AML-12 mouse normal hepatocytes^[1].
NLRP3-IN-91 (0.2-5 μM; 2.5 h) dose-dependently inhibits NLRP3 inflammasome assembly and activation in J774A.1 murine macrophages by reducing cleaved caspase-1 (p20) and mature IL-1β production^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[1]

Cell Line:

J774A.1 murine macrophages
Concentration:

0.04, 0.12, 0.37, 1.11, 3.33, 10 μM
Incubation Time:

30 min
Result:

Inhibited IL-1β secretion by 80.2% at 1 μM.
Inhibited IL-1β secretion by 96.6% at 10 μM.
Exhibited an IC₅₀ of 0.094 μM for NLRP3 inflammasome-mediated IL-1β release.

Western Blot Analysis^[1]

Cell Line:

J774A.1 murine macrophages
Concentration:

0.2, 1, and
5 μM
Incubation Time:

2.5 h
Result:

Suppressed the production of cleaved caspase-1 (p20 subunit) and mature IL-1β in a dose-dependent manner.
Did not affect levels of pro-caspase-1, pro-IL-1β, NLRP3, or GAPDH.

In Vivo

NLRP3-IN-91 (compound D12) (10 mg/kg; i.p.; single dose; 1 hour pre-LPS) significantly prolongs the survival time of LPS-induced septic mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male C57BL/6 mice (8 weeks old, 20-25 g) intraperitoneally injected with LPS (40 mg/kg)^[1]
Dosage:

10 mg/kg
Administration:

i.p.; single dose; 1 hour pre-LPS
Result:

Exceeded 40% survival rate at 72 hours post-LPS challenge.

Chemical Information

Molecular Weight 576.04
Formel C₂₇H₂₇ClFN₃O₆S
SMILES

O=C(N(CC1=CC(Cl)=CC=C1OCCOC)C2=NC3=CC(NS(=O)(CC)=O)=CC=C3O2)CC4=CC=C(F)C=C4
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Shi Z, et al. Design, synthesis, and anti-inflammatory evaluation of novel N-(Benzol[d]oxazol-5-yl)sulfonamides as NLRP3 inflammasome inhibitors. Bioorg Chem. 2026;177:109904. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Reinheit & Dokumentation
Verweise

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NLRP3-IN-91

Alle NOD-like Receptor (NLR) Isoform-spezifische Produkte anzeigen

Alle Interleukin Related Isoform-spezifische Produkte anzeigen

Alle Caspase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

NLRP3-IN-91 Related Classifications

HY-183296 Related Classifications