NS2B/NS3-IN-3 hydrochloride
Based on 1 Customer Validation
NS2B/NS3-IN-3 hydrochloride is an inhibitor of Flavivirus NS2B-NS3 protease, with an IC50 of 0.32 μM against Zika virus NS2B-NS3 protease. NS2B/NS3-IN-3 hydrochloride also exhibits inhibitory effects on dengue virus type 2 NS2B-NS3 protease and West Nile virus NS2B-NS3 protease. NS2B/NS3-IN-3 hydrochloride inhibits the intracellular replication of Zika virus. NS2B/NS3-IN-3 hydrochloride can be used in studies related to flavivirus infections.
For research use only. We do not sell to patients.
- Purity: 97.01%
- CAS No.: 2832876-91-0
- Formula: C19H22ClN3O2
- Molecular Weight:359.85
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
NS2B/NS3-IN-3 (compound 66) hydrochloride potently inhibits recombinant Zika virus NS2B-NS3 protease with an IC50 of 0.32 μM; it also inhibits recombinant dengue virus type 2 NS2B-NS3 protease with an IC50 of approximately 1-2 μM, and exhibits a non-competitive inhibition mode[1].
NS2B/NS3-IN-3 (1-10 μM; 48 h) hydrochloride exhibits dose-dependent antiviral activity against Zika virus (strain FLR) in human U87 glioma cells, with an EC68 of 1 μM, and shows no toxicity to U87 cells at a concentration of 10 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2832876-91-0
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Appearance Solid
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Molecular Weight 359.85
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Formula C19H22ClN3O2
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Color Orange to red
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SMILES
O=C(C1=CC2=CC=C(C=C2N1)C3=COC=C3)NCC4CCNCC4.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 10 mg/mL (27.79 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (2.78 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7789 mL | 13.8947 mL | 27.7894 mL | 69.4734 mL |
| 5 mM | 0.5558 mL | 2.7789 mL | 5.5579 mL | 13.8947 mL | |
| 10 mM | 0.2779 mL | 1.3895 mL | 2.7789 mL | 6.9473 mL | |
| 15 mM | 0.1853 mL | 0.9263 mL | 1.8526 mL | 4.6316 mL | |
| 20 mM | 0.1389 mL | 0.6947 mL | 1.3895 mL | 3.4737 mL | |
| 25 mM | 0.1112 mL | 0.5558 mL | 1.1116 mL | 2.7789 mL |
NS2B/NS3-IN-3 hydrochloride Related Classifications
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