Okadaic acid sodium
Based on 24 publication(s) in Google Scholar
Okadaic acid sodium, a marine toxin, is an inhibitor of protein phosphatases (PP). Okadaic acid (sodium) has a significantly higher affinity for PP2A (IC50=0.1-0.3 nM), and inhibits PP1 (IC50=15-50 nM), PP3 (IC50=3.7-4 nM), PP4 (IC50=0.1 nM), PP5 (IC50=3.5 nM), but does not inhibit PP2C. Okadaic acid sodium increases of phosphorylation of a number of proteins by inhibiting PP, and acts a tumor promoter. Okadaic acid sodium induces tau phosphorylation.
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- CAS No.: 209266-80-8
- 화학식: C44H67NaO13
- 분자량:826.98
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보관:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Okadaic acid sodium
More- Science. 2026 Jan 15;391(6782):eadq9006. [Abstract]
- Bone Res. 2025 Jan 2;13(1):3. [Abstract]
- Adv Sci (Weinh). 2026 Mar 12:e22837. [Abstract]
- Biomaterials. 2025 Jan:312:122749. [Abstract]
- Cancer Lett. 2021 May 28:506:67-82. [Abstract]
- Cell Commun Signal. 2024 Aug 7;22(1):391. [Abstract]
- Int J Biol Macromol. 2025 Sep 24;329(Pt 2):147874. [Abstract]
- Int J Biol Macromol. 2023 Jul 1;242(Pt 4):125171. [Abstract]
- NPJ Parkinsons Dis. 2026 Jun 6. [Abstract]
- Cell Rep. 2026 Mar 20;45(4):117130. [Abstract]
- BioChip J. 2025 Aug 1.
- Int Immunopharmacol. 2024 Sep 30:139:112680. [Abstract]
- Mol Med Rep. 2024 Sep;30(3):155. [Abstract]
- Cell Signal. 2026 Mar:139:112333. [Abstract]
- Mol Divers. 2025 Jul 13. [Abstract]
- Transcription. 2021 Oct;12(5):277-293. [Abstract]
- Int J Biochem Cell Biol. 2021 Aug:137:106036. [Abstract]
- Mol Cell Endocrinol. 2025 Aug 28:609:112645. [Abstract]
- Biochem Biophys Res Commun. 2023 Nov 5:680:127-134. [Abstract]
- Heidelberg University. 2025.
- Precision medication. 2025 Sep 17;2(3):100050.
- University of Padova. 2025.
- bioRxiv. 2023 Jun 6.
- Research Square Preprint. 2022 Mar.
Customer Validation & Images
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WB
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IP
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Flow Cytometry
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Cell Imaging/Staining
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IF
Biological Activity
제품 설명
IC50 & Target
[1]|
PP1 15-50 nM (IC50) |
PP2A 0.1-0.3 nM (IC50) |
PP3 3.7-4 nM (IC50) |
PP4 0.1 nM (IC50) |
PP5 3.5 nM (IC50) |
PP2B ~4000 nM (IC50) |
PP7 >1000 nM (IC50) |
In Vitro
Okadaic acid sodium (0-100 nM; 24 h or 48 h) inhibits the proliferation of AGS, MNK-45, Caco 2 cells[3].
Okadaic acid sodium (10 nM; 8 hours) increases Drp1 phosphorylation and mitochondrial fission in rat cortical neurons[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:AGS, MNK-45 and Caco 2 cell lines
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Concentration:0-100 nM
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Incubation Time:24 h or 48 h
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Result:Inhibited the proliferation of AGS, MNK-45, Caco 2 cells.
Induced Disease Models
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.
Okadaic acid sodium can be used to induce Alzheimer's disease models[6].
Administration: 100μg • icv • single dose
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 209266-80-8
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Appearance Oil
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분자량 826.98
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화학식 C44H67NaO13
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Color Colorless to light yellow
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SMILES
O[C@H](CC[C@@H](C[C@@](C)(O)C(O[Na])=O)O1)[C@@]1(C=C(C)C2)O[C@]2([H])[C@H](C)/C=C/[C@@H]3O[C@@]4(O[C@]([C@@H]5O)([H])[C@@](O[C@@]([C@@H](O)C[C@@H]([C@@]6([H])O[C@@]7(CCCCO7)CC[C@H]6C)C)([H])C5=C)([H])CC4)CC3
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (24)
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Journal Impact Factor
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Most Recent
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Science
2026 Jan 15;391(6782):eadq9006. PMID: 41538457 -
Bone Res
MYSM1 attenuates osteoarthritis by recruiting PP2A to deubiquitinate and dephosphorylate RIPK2. [Abstract]2025 Jan 2;13(1):3. PMID: 39746943
Okadaic acid sodium purchased from MedChemExpress. Usage Cited in: Bone Res. 2025 Jan 2;13(1):3. [Abstract]
Western blots of p-PP2Ac, PP2Ac, p-RIPK2, and RIPK2 in mouse chondrocytes transfected with Mysm1with or without Okadaic Acid (10 μM) treatment.
Okadaic acid sodium purchased from MedChemExpress. Usage Cited in: Bone Res. 2025 Jan 2;13(1):3. [Abstract]
Immunoprecipitation assay showing RIPK2 ubiquitination after Okadaic Acid(10 μM) treatment.
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Adv Sci (Weinh)
Detachment-Induced FAK-STAT3-NNMT Inhibits CTCs Anoikis to Promote Breast Cancer Metastasis by Enhancing Fatty Acid Oxidation. [Abstract]2026 Mar 12:e22837. PMID: 41816955 -
Biomaterials
Highly BBB-permeable nanomedicine reverses neuroapoptosis and neuroinflammation to treat Alzheimer's disease. [Abstract]2025 Jan:312:122749. PMID: 39121725
Okadaic acid sodium purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 Jan:312:122749. [Abstract]
Okadaic acid (30 nM; 24 h). Flow cytometry analysis of intracellular ROS in PC12 cells after different treatments.
Okadaic acid sodium purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 Jan:312:122749. [Abstract]
Okadaic acid (30 nM; 24 h). Live/dead fluorescence images of PC12 cells after different treatments.
Okadaic acid sodium purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 Jan:312:122749. [Abstract]
Okadaic acid (100 μM; 130 nL). Immunofluorescence staining of CD86− and Iba-1 positive cells in the cortex of mice subjected to different treatments.
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Cancer Lett
2021 May 28:506:67-82. PMID: 33675985 -
Cell Commun Signal
Concomitant targeting of FLT3 and SPHK1 exerts synergistic cytotoxicity in FLT3-ITD+ acute myeloid leukemia by inhibiting β-catenin activity via the PP2A-GSK3β axis. [Abstract]2024 Aug 7;22(1):391. PMID: 39113090 -
Int J Biol Macromol
Multi-omics integration reveals key miRNAs, immune inflammation, and signaling pathways in Alzheimer's disease: Implications for targeted therapy. [Abstract]2025 Sep 24;329(Pt 2):147874. PMID: 41005404 -
Int J Biol Macromol
2023 Jul 1;242(Pt 4):125171. PMID: 37271265 -
NPJ Parkinsons Dis
Metabotropic signaling downstream of GABAA receptors suppresses neuroinflammation in Parkinson's disease. [Abstract]2026 Jun 6. PMID: 42251036 -
Cell Rep
Autoinhibitory control of MLKL governs pseudokinase domain phosphorylation and oligomerization during necroptosis. [Abstract]2026 Mar 20;45(4):117130. PMID: 41863805 -
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Int Immunopharmacol
LincR-PPP2R5C regulates IL-1β ubiquitination in macrophages and promotes airway inflammation and emphysema in a murine model of COPD. [Abstract]2024 Sep 30:139:112680. PMID: 39018689 -
Mol Med Rep
Identification and immunological characterization of genes associated with ferroptosis in Alzheimer's disease and experimental demonstration. [Abstract]2024 Sep;30(3):155. PMID: 38963039 -
Cell Signal
PERK-eIF2alpha-mediated translational inhibition of MCL-1 contributes to potential 2-deoxy-D-glucose and BAD mimetic combinatorial cancer therapy. [Abstract]2026 Mar:139:112333. PMID: 41423011 -
Mol Divers
Synthesis and multi-target evaluation of 2-(2-phenylethyl)/2,3-styrylchromone derivatives as potential anti-Alzheimer's disease agents. [Abstract]2025 Jul 13. PMID: 40652414 -
Transcription
Oxidative stress induces Ser 2 dephosphorylation of the RNA polymerase II CTD and premature transcription termination. [Abstract]2021 Oct;12(5):277-293. PMID: 34874799 -
Int J Biochem Cell Biol
Modified 5-aminolevulinic acid photodynamic therapy (M-PDT) inhibits cutaneous squamous cell carcinoma cell proliferation via targeting PP2A/PP5-mediated MAPK signaling pathway. [Abstract]2021 Aug:137:106036. PMID: 34217813 -
Mol Cell Endocrinol
The N-terminal region of HASPIN regulates phosphorylation of AURKA and meiotic progression in spermatocytes. [Abstract]2025 Aug 28:609:112645. PMID: 40885374 -
Biochem Biophys Res Commun
Engrailed 2 triggers the activation of multiple phosphorylation-induced signaling pathways in both transcription-dependent and -independent manners. [Abstract]2023 Nov 5:680:127-134. PMID: 37738902 -
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Protocol
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Kinase activity and phosphorylation assays
Kinase activity assays measure the ability of kinases to transfer phosphate groups from ATP to specific substrates, while phosphorylation assays detect the presence and levels of phosphorylated proteins. Common methods include radiolabeled ATP incorporation (e. g. ,), ADP release detection via bioluminescence (e. g. ,[3]), enzyme-linked immunosorbent assays (ELISA) for phospho-specific epitopes (e. g. ,[6]), and microtiter-based formats for high-throughput screening (e. g. ,[8]). The ADP-Glo assay quantifies kinase activity by measuring ADP produced during phosphorylation using a luciferase-based system. Radiometric assays involve autoradiography or scintillation counting after incorporation of 32P-labeled ATP into substrate proteins. ELISA-based approaches rely on phospho-specific antibodies to detect activated kinases in cell lysates or purified samples.
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Western Blot
Western blotting (WB) is a commonly used experimental method in molecular biology, biochemistry, and immunogenetics for identifying and quantifying target proteins. It combines gel electrophoresis with immunoassay, enabling researchers to analyze protein expression, post-translational modifications, and molecular weight.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Protocol for Kinase activity and phosphorylation assays
Kinase activity assays measure transfer of phosphate from ATP to a protein or peptide substrate, generating phosphorylated substrate, ADP, or incorporated radiolabeled phosphate as the readout; phosphorylation assays measure site-specific phosphorylation in cells or tissues as a proxy for kinase-pathway activation, inhibition, or substrate regulation. Phosphorylation can be detected by phospho-specific Western blot, immunoprecipitation kinase assay, phospho-immunofluorescence, phospho-flow cytometry, luminescent ADP detection, radiolabeled ATP incorporation, or reporter-based pathway assays, and these readouts can be applied to cancer cells, primary neurons, mouse tumors, organoids, inflammatory macrophages, ferroptosis studies, and mitophagy studies when the kinase target is biologically relevant.
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Alzheimer’s Disease Modeling
Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by a progressive decline in cognitive functions and loss of specific types of neurons and synapses. Alzheimer's symptoms can be simulated in mice by injecting drugs (such as Aβ) or genetically modified.
순도&문서
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Data Sheet (275 KB)
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SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Korean - KR (420 KB)
- Portuguese - PT (420 KB)
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Handling Instructions (2659 KB)
References
[1]. Kleppe R, et al. Cell Death Inducing Microbial Protein Phosphatase Inhibitors--Mechanisms of Action. Mar Drugs. 2015 Oct 22;13(10):6505-20. [Content Brief]
[2]. Valdiglesias V, et al. Okadaic acid: more than a diarrheic toxin. Mar Drugs. 2013 Oct 31;11(11):4328-49. [Content Brief]
[3]. del Campo M, et al. Okadaic acid toxin at sublethal dose produced cell proliferation in gastric and colon epithelial cell lines. Mar Drugs. 2013;11(12):4751-4760. [Content Brief]
[4]. Cho MH, et al. Increased phosphorylation of dynamin-related protein 1 and mitochondrial fission in okadaic acid-treated neurons. Brain Res. 2012 May 15;1454:100-10. [Content Brief]
[5]. Baker S, et al. A local insult of okadaic acid in wild-type mice induces tau phosphorylation and protein aggregation in anatomically distinct brain regions. Acta Neuropathol Commun. 2016;4:32. [Content Brief]
[6]. Natalia Dos Santos Tramontin, et al. Gold Nanoparticles Treatment Reverses Brain Damage in Alzheimer's Disease Model . Mol Neurobiol. 2020, 57, 2. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Okadaic acid sodium Related Classifications
HY-N6785A Related Classifications
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